Influence of a Novel Surface of Bioactive Implants on Osseointegration: A Comparative and Histomorfometric Correlation and Implant Stability Study in Minipigs

Purpose: The objective of this study was to assess the influence of a novel surface of dental implants (ContacTi®) on the osseointegration process in a minipig model. The surface was compared with other existing surfaces on the market (SLA® and SLActive®) by employing bone implant contact analysis (BIC) and implant stability. Method: Twelve minipigs were used with prior authorisation from an ethics committee. Three types of surfaces were tested: SLA® (sand-blasted acid-etched titanium), SLActive® (same but hydrophilic, performed under a nitrogen atmosphere), and ContacTi® (alumina particle bombardment of titanium, bioactivated when treated thermochemically) in 4.1 mm × 8 mm implants with internal connection and a polished neck. Twelve implants of each surface type (N = 36) were placed, sacrificing 1/3 of the animals at 2 weeks of placement, 1/3 at 4 weeks and the remaining 1/3 at 8 weeks. Numerical variables were compared with Analysis of Variance, and the correlation between ISQ and BIC was established with the Spearman’s rank correlation coefficient. Results: SLActive® and ContacTi® surfaces showed elevated osteoconductivity at 4 weeks, maintaining a similar evolution at 8 weeks (large amount of mature lamellar tissue with high maturity and bone quality). The SLA® surface showed slower maturation. The ISQ values in surgery were elevated (above 65), higher at necropsy and higher at 4 and 8 weeks in the SLA® group than in the other two (SLActive® and ContacTi®). No significant correlation was found between ISQ and BIC for each implant surface and necropsy time. Conclusion: The three surfaces analysed showed high RFA and BIC values, which were more favourable for the SLActive® and ContacTi® surfaces. No statistical correlation was found between the RFA and BIC values in any of the three surfaces analysed.Research Foundation of the University of Seville Project Code 187

Co-Existence of Inoculated Yeast and Lactic Acid Bacteria and Their Impact on the Aroma Profile and Sensory Traits of Tempranillo Red Wine

This study investigates the effects of simultaneous inoculation of a selected Saccharomyces cerevisiae yeast strain with two different commercial strains of wine bacteria Oenococcus oeni at the beginning of the alcoholic fermentation on the kinetics of malolactic fermentation (MLF), wine chemical composition, and organoleptic characteristics in comparison with spontaneous MLF in Tempranillo grape must from Castilla-La Mancha (Spain). Evolution of MLF was assessed by the periodic analysis of L-malic acid through the enzymatic method, and most common physiochemical parameters and sensory traits were evaluated using a standardized sensory analysis. The samples were analyzed by GC/MS in SCAN mode using a Trace GC gas chromatograph and a DSQII quadrupole mass analyzer. Co-inoculation reduced the overall fermentation time by up to 2 weeks leading to a lower increase in volatile acidity. The fermentation-derived wine volatiles profile was distinct between the co-inoculated wines and spontaneous MLF and was influenced by the selected wine bacteria used in co-inoculation. Co-inoculation allows MLF to develop under reductive conditions and results in wines with very few lactic and buttery flavors, which is related to the impact of specific compounds like 2,3-butanedione. This compound has been also confirmed as being dependent on the wine bacteria use

Bacteriophages as an Up-and-Coming Alternative to the Use of Sulfur Dioxide in Winemaking

Certain acetic and lactic acid bacteria are major causes of quality defects in musts and wines, giving rise to defects such as a "vinegary," "sharp, like nail polish-remover" taste or preventing alcoholic and/or malolactic fermentation. Sulfur dioxide is the major tool currently used in the control of these bacteria in wine. The aim of this work was to isolate bacteriophages from musts and wine of different grape varieties that were able to eliminate lactic and acetic acid bacteria spoilages at the laboratory scale. Musts obtained from grape-berries of Vitis vinifera cv. Chardonnay and Moscatel and a red wine made with V. vinifera cv. Tintilla de Rota were used to isolate bacteriophages. Bacteriophages were obtained from each of the musts and the wine and belonged to the order Caudovirals and the family Tectivirals. They were isolated by classical virology methods and identified by electron microscopy. The host bacteria used in the study were lactic acid bacteria of the species Lactobacillus hilgardii, Lactobacillus plantarum, and Oenococcus oeni and the acetic bacteria Acetobacter aceti. A comparative study was performed by adding phage titrations and SO2 to musts and wines, which had been previously inoculated with bacteria, to study the effectiveness of bacteriophages against bacteria. The comparative study showed that some bacteriophages were as effective as sulfur dioxide at low concentration

La dación de fe como acto propio

Si bien es verdad que podemos remontar al notariado como sucesores de los escribas, también en cierto que desde la antigüedad no existe un concepto claro sobre la actividad notarial en especial sobre el acto de fe notarial, el cual ha sido asimilado al acto administrativo o al judicial, confusión que hemos decidido terminar o por lo menos aclarar. Así, mediante esta tesis analizaremos teorías de los actos administrativos y judiciales, tratando de estudiar los conceptos del uno y del otro; al igual que definir lo que es el acto de fe notarial. Tenemos como objeto de este estudio, el demostrar que no es lo mismo el acto administrativo, el judicial ni el notarial. Y como uno de los objetivos específicos el determinar un concepto de fe notarial como acto propio, para lo cual utilizaremos un método de investigación netamente legal y doctrinarioLa fe pública es un concepto tan genérico que es muy probable que se pierda en su contenido, al no existir una definición legal. Tendremos a base de lo manda en el art. 18 del C.C. llegar a la interpretación gramatical o la que dan quienes practican la ciencia, arte u oficio, lo cual es complicado ya que la fe pública no solo la da u otorga el notario, sino también todo funcionario de fe como puede ser un citador judicial, podríamos concretarla como el criterio que genera veracidad y certeza, tanto como tener apariencia real, como ser producto o emanado de un funcionario competente, es decir, lo mismo que la fe privada pero otorgada por un funcionario calificado, el funcionario calificado y tal vez el más conocido es el notario. Quien produce y crea fe pública como también la reproduce, todo esto lo logra mediante el Acto Notarial que es el ejercicio mediante el cual se materializa a través de instrumentos físicos las facultades que la Ley otorga al notario. Así pues el acto notarial es la práctica de la ciencia notarial mediante la prueba escrita en legal y debida forma de los actos, contratos sean estos solemnes o no solemnes requeridos por parte capaz y libre, en la circunscripción territorial en la cual tiene competencia el notario

Web 2.0 Tools for RolePlay Methodology in an Undergraduate Interdisciplinary Environment

El objetivo de este trabajo es, en primer lugar, proponer una actividad a través de la metodología activa denominada juego de rol en un contexto interdisciplinar, esto es, una actividad que aboga por la interacción de alumnos con roles profesionales específicos de diferentes disciplinas, como bien demanda la sociedad actualmente. En segundo lugar, se hace especial hincapié en la integración de herramientas digitales de la llamada Web 2.0, concretamente, en el uso de wikis, blogs y podcasts para poder llevar a cabo de forma satisfactoria el juego de rol interdisciplinar propuesto.The main aim of this work is, first of all, to propose an activity by means of a the active methodology called roleplay in an interdisciplinary context, that is to say, an activity that pleads for the interaction of undergraduate students with specific professional roles from different disciplines, as current society demands. Secondly, we will pay special attention to the integration of digital tools of the so called Web 2.0, mainly, to the use of the wikis, blogs, and podcasts so as to carry out successfully the proposed interdisciplinary roleplay task

Choline kinase inhibitors EB-3D and EB-3P interferes with lipid homeostasis in HepG2 cells

A full understanding of the molecular mechanism of action of choline kinase α (ChoKα) inhibitors at the cell level is essential for developing therapeutic and preventive approaches for cancer. The aim of the present study was to evaluate the effects of the ChoKα inhibitors EB-3D and EB-3P on lipid metabolism in HepG2 cells. We used [methyl-14C]choline, [1,2-14C]acetic acid and [2-3H]glycerol as exogenous precursors of the corresponding phospholipids and neutral lipids. [Methyl-14C]choline was also used to determine choline uptake. Protein levels were determined by Western blot. Ultrastructural alterations were investigated by transmission electron microscopy. In this work, we demonstrate that EB-3D and EB-3P interfere with phosphatidylcholine biosynthesis via both CDP-choline pathway and choline uptake by the cell. Moreover, the synthesis of both diacylglycerols and triacylglycerols was affected by cell exposure to both inhibitors. These effects were accompanied by a substantial decrease in cholesterol biosynthesis, as well as alterations in the expression of proteins related to cholesterol homeostasis. We also found that EB-3D and EB-3P lowered ChoKα protein levels. All these effects could be explained by the modulation of the AMP-activated protein kinase signalling pathway. We show that both inhibitors cause mitochondrial alteration and an endoplasmic reticulum stress response. EB-3D and EB-3P exert effects on ChoKα expression, AMPK activation, apoptosis, endoplasmic reticulum stress and lipid metabolism. Taken together, results show that EB-3D and EB-3P have potential anticancer activity through the deregulation of lipid metabolism.This work was aided by the Andalusian Regional Government (P11-CVI-7859). The inhibitors were developed under the Cei-Biotic Project CEI2013-MP-1 (University of Granada)

Isolation of bacteriophages from must and wine for the elimination of contaminating bacteria as an alternative to the use of sulfurous

Acetic and some lactic acid bacteria are the main reason for the loss of quality of musts and wines, giving rise to defects such as "vinegary", "chopped" or preventing alcoholic and / or malolactic fermentation. The solution to this problem consists in the application of authorized bactericidal agents, such as sulfurous. The aim of this work is to isolate bacteriophages from musts and wines of different grape varieties, able to eliminate lactic and acetic acid bacteria spoilages. Musts obtained from grape-berries of Vitis vinifera cv. Chardonnay and Moscatel and a red wine made with the Tintilla de Rota variety were used to isolate bacteriophages. It were isolated by classical virology methods and identified by electron microscopy. Host bacteria used in the study were the lactic acid bacteria of the species Lactobacillus hilgardii, L. plantarum and Oenococcus oeni and the acetic bacteria Acetobacter aceti. A comparative study was performed on musts and wines, previously inoculated with bacteria, by phage titration and SO2 addition to study the effectiveness of bacteriophages against bacteria. Bacteriophages were obtained from all musts and wine, belonging to the order of Caudovirals and Tectivirals. The comparative study showed that a cocktail of bacteriophages at low concentration is as effective as sulfur

Assessment of the Simultaneous Use of Biomaterials in Transalveolar Sinus Floor Elevation: Prospective Randomized Clinical Trial in Humans

Implants inserted in the posterior maxilla frequently need additional surgery for successful bone augmentation. One of the most common procedures for this is transalveolar sinus floor elevation. There are di erent protocols for this procedure, and there is controversy over the simultaneous application of grafting material upon elevating. In this prospective randomized clinical study in humans, a total of 49 transalveolar sinus floor elevations were performed in 49 di erent patients, divided into a control group (without graft, 25 patients) and a test group (with graft, 24 patients). The analyzed variables were obtained through digital orthopantomography on day 0 (day of surgery) and 18 months after surgery. These measurements showed a tendency towards greater vertical bone gain in the test group, but this was not statistically significant. Therefore, considering that sinus elevation and implant placement without the application of grafts is a successful treatment with fewer complications, a critical assessment of the need for these biomaterials is necessary

Design, synthesis, crystallization and biological evaluation of new symmetrical biscationic compounds as selective inhibitors of human Choline Kinase α1 (ChoKα1)

The authors gratefully acknowledge the ‘Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía’ (Excellence Research Project: P07-CTS-03210), the ‘Diputación General de Aragón (B89)’ and the ‘Ministerio de Ciencia e Innovación’ (SAF2009-11955, BFU2010-19504 and CTQ2013-44367-C2-2-P) for the financial support, the award of grants from ‘Ministerio de Educación’ to P.R.-M. and S.S.-E. is gratefully acknowledged, and the ‘Centro de Servicios de Informática of the University of Granada (Spain) for the use of their computers and scientific software. G.V.,R.B., R.M. and G.B. We thanks also the Fondazione Cariparo by the “Progetto Ricerca Pediatrica”. We thank synchrotron radiation sources ALBA (Barcelona), and in particular the beamline XALOC. The research leading to these results has also received funding from the FP7 (2007–2013) under BIOSTRUCTX-7687.A novel family of compounds derivative of 1,1′-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))-bispyridinium or –bisquinolinium bromide (10a-l) containing a pair of oxygen atoms in the spacer of the linker between the biscationic moieties, were synthesized and evaluated as inhibitors of choline kinase against a panel of cancer-cell lines. The most promising compounds in this series were 1,1′-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))bis(4-(dimethylamino)pyridinium) bromide (10a) and 1,1′-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))-bis(7-chloro-4-(pyrrolidin-1-yl)quinolinium) bromide (10l), which inhibit human choline kinase (ChoKα1) with IC50 of 1.0 and 0.92 μM, respectively, in a range similar to that of the previously reported biscationic compounds MN58b and RSM932A. Our compounds show greater antiproliferative activities than do the reference compounds, with unprecedented values of GI50 in the nanomolar range for several of the cancer-cell lines assayed, and more importantly they present low toxicity in non-tumoral cell lines, suggesting a cancer-cell-selective antiproliferative activity. Docking studies predict that the compounds interact with the choline-binding site in agreement with the binding mode of most previously reported biscationic compounds. Moreover, the crystal structure of ChoKα1 with compound 10a reveals that this compound binds to the choline-binding site and mimics HC-3 binding mode as never before.Junta de Andalucía P07-CTS-03210Diputación General de Aragón (B89)Ministerio de Ciencia e Innovación (SAF2009-11955, BFU2010-19504, CTQ2013-44367-C2-2-P)Ministerio de EducaciónUniversity of Granada (Spain)Fondazione CariparoBIOSTRUCTX-7687 FP7 (2007–2013

Interdisciplinary Tutoring for the Development of Professional-Simulation Role-Plays

Current society is characterised by a growing tendency of interrelationship among different professional sectors in order to offer their potential customers a better quality of certain products or process. Thereby, and in the context of this interdisciplinarity, higher-education teachers must frame their teaching methodologies mainly focused on the acquisition of certain competences so that students could guarantee the development of their professional abilities. Taking this as a premise, we have performed an interdisciplinary role-play so as to allow our students to acquire those professional competences. However, to carry out this performance a well-organised tutorial plan, divided into several tutoring sessions, was required to succeed in the use of this active methodology