ANTIDIABETIC POTENTIAL OF THE OYSTER MUSHROOM PLEUROTUS FLORIDA (MONT.) SINGER

Objective: The present investigation comprises, in vitro antidiabetic activity such as α-amylase and α-glucosidase inhibitory activities and in vivo antidiabetic activity of methanolic extract of Pleurotus florida.Methods: The fruiting bodies of Pleurotus florida were obtained from Mushroom Unit, Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu, India. Sample preparation, qualitative phytochemical analysis, in vitro antidiabetic activities namely α-amylase and α-glucosidase inhibitory activity and in vivo antidiabetic activity namely evaluation of alloxan induced diabetic rats were carried out following the methods reported previously.Results: In vitro and in vivo antidiabetic activity of P. florida exhibited significant results for its α-amylase (94.93±1.75 % at 1000 µg/ml) and α-glucosidase inhibitory activity (84.90±0.42 % at 1000 µg/ml) in a dose-dependent manner. The extract also showed significant antidiabetic activity on in vivo (p<0.05) at the tested dose level (200 mg/kg b. w) this was comparable to Glibenclamide, a standard antidiabetic drug.Conclusion: The presence of phytochemicals namely phenols, flavonoids, saponins, tannins and terpenoids may be responsible for such antidiabetic activity. These results reveal that P. florida can be used as a potential antidiabetic agent

MYCOSYNTHESIS OF SILVER NANOPARTICLES: CHARACTERIZATION, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY FROM PLEUROTUS FLORIDA (MONT) SINGER: A MACRO FUNGI

  Objective: To determine the antioxidant and anti-inflammatory activity of myco-synthesized silver nanoparticle (AgNP) from Pleurotus florida. Methods: Fresh mushrooms were collected, processed, and the AgNPs were synthesized using standard reducing agent 1 mM of silver nitrate. The characteristics of synthesized particles were confirmed using ultraviolet, Fourier transmission infrared, X-ray diffraction, and energy dispersive X-ray analysis. These nanoparticles were subjected for antioxidant activity through 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and anti-inflammatory activity using bovine serum albumin denaturation assay, respectively, and the percentage of inhibition of AgNPs was calculated with standard error mean.Results: The AgNPs were recorded the absorbance band at 410 nm was the particle size of 20-50 nm with a rod in shape. In DPPH assay the antioxidant activity was recorded as 96.2% with 50% inhibitory concentration (IC50) value of 85 μg/ml. In anti-inflammatory activity, the activity was recorded as 92.10±0.005% and IC50 value was recorded as 100 μg/ml.Conclusion: The results indicated the significant antioxidant and anti-inflammatory activity of AgNPs from P. florida. Hence, further in vivo studies are focused to confirm the anticancer and anti-inflammatory activities

Antimicrobial Activity of Mushrooms against Skin Infection Causing Pathogens

Mushrooms are nutritionally functional food and a source of physiologically beneficial and nontoxic medicines for various diseased conditions. In the present study antimicrobial properties of crude extracts of three commercial edible mushrooms (Agaricus bisporus (J.E.Lange) Imbach , Pleurotus florida (Mont.) and Calocybe indica (P&A) were tested against bacteria and fungi that cause local dermatitis by disc diffusion method. Highest anti-microbial activity was obtained from petroleum ether extract of Agaricus bisporus, with the zone of inhibition 17mm (Streptococus pyogenes), 15mm (Staphylococus aureus), 14mm (Pseudomonas aeuroginosa) and 13mm (Candida albicans) from 100µg/ml concentration of mushroom extracts respectively, while minimal zone was obtained from the petroleum ether extract of Pleurotus florida and very least inhibition was observed in Calocybe indica. From the results it is inferred that crude extracts of commercially available mushrooms namely Agaricus bisporus and Pleurotus florida can be used to treat pathogenic microbes that cause skin irritations, bristles and acnes. This study gives scope for the investigations on active constituents of mushrooms for better understanding of the healing mechanism