A multi-organ maize metabolic model connects temperature stress with energy production and reducing power generation

Climate change has adversely affected maize productivity. Thereby, a holistic understanding of metabolic crosstalk among its organs is important to address this issue. Thus, we reconstructed the first multi-organ maize metabolicmodel, iZMA6517, and contextualized itwith heat and cold stress transcriptomics data using expression distributed reaction flux measurement (EXTREAM) algorithm. Furthermore, implementing metabolic bottleneck analysis on contextualized models revealed differences between these stresses. While both stresses had reducing power bottlenecks, heat stress had additional energy generation bottlenecks.We also performed thermodynamic driving force analysis, revealing thermodynamics-reducing power-energy generation axis dictating the nature of temperature stress responses. Thus, a temperaturetolerant maize ideotype can be engineered by leveraging the proposed thermodynamics-reducing powerenergy generation axis.We experimentally inoculated maize root with a beneficial mycorrhizal fungus, Rhizophagus irregularis, and as a proof-of-concept demonstrated its efficacy in alleviating temperature stress. Overall, this study will guide the engineering effort of temperature stress-tolerant maize ideotypes

Nitrates : De l'azote minéral à la matière organique

International audienc

The challenge of remobilisation in plant nitrogen economy. A survey of physio-agrobnomic and molecular approaches

International audienc

The challenge of remobilisation in plant nitrogen economy. A survey of physio-agrobnomic and molecular approaches

International audienc

Utilisation de la transgenese pour l'analyse du metabolisme du nitrate

National audienc

Introduction and expression of a deregulated tobacco nitrate reductase gene in potato lead to highly reduced nitrate levels in transgenic tubers

International audienc

An artificial productive ecosystem based on a fish/bacteria/plant association. 1. Design and management

International audienc

An artificial productive ecosystem based on a fish/bacteria/plant association. 1. Design and management

International audienc

The effects of deregulation of NR gene expression on growth and nitrogen metabolism of Nicotiana plumbaginifolia plants

International audienc