Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk

Human-like H3N2 influenza viruses have repeatedly been transmitted to domestic pigs in different regions of the world, but it is still uncertain whether any of these variants could become established in pig populations. The fact that different subtypes of influenza viruses have been detected in pigs makes them an ideal candidate for the genesis of a possible reassortant virus with both human and avian origins. However, the determination of whether pigs can act as a “mixing vessel” for a possible future pandemic virus is still pending an answer. This prompted us to gather the epidemiological information and investigate the genetic evolution of swine influenza viruses in Jilin, China.Nasopharyngeal swabs were collected from pigs with respiratory illness in Jilin province, China from July 2007 to October 2008. All samples were screened for influenza A viruses. Three H3N2 swine influenza virus isolates were analyzed genetically and phylogenetically.Influenza surveillance of pigs in Jilin province, China revealed that H3N2 influenza viruses were regularly detected from domestic pigs during 2007 to 2008. Phylogenetic analysis revealed that two distinguishable groups of H3N2 influenza viruses were present in pigs: the wholly contemporary human-like H3N2 viruses (represented by the Moscow/10/99-like sublineage) and double-reassortant viruses containing genes from contemporary human H3N2 viruses and avian H5 viruses, both co-circulating in pig populations.The present study reports for the first time the coexistence of wholly human-like H3N2 viruses and double-reassortant viruses that have emerged in pigs in Jilin, China. It provides updated information on the role of pigs in interspecies transmission and genetic reassortment of influenza viruses

Evaluation Method of Soil Surface Roughness after Ditching Operation Based on Wavelet Transform

Soil surface roughness (SSR) is an important parameter affecting surface hydrology, erosion, gas exchange and other processes. The surface roughness of the farmland environment is directly related to the tillage process. In order to accurately characterize the random roughness (RR) parameters of the surface after ditching, a three-dimensional (3D) digital model of the surface was obtained by laser scanning under the conditions of an indoor ditching test, and the influence of oriented roughness components formed by removing ridge characteristics on the RR of the surface was analyzed by introducing the wavelet processing method. For this reason, four groups of ditching depths and two types of surface conditions (whether the surface was agglomerated or not) were designed in this paper. By comparing the root mean squared height (RMSH) and correlation length (CL) data calculated before and after wavelet processing under each group of tests, it was concluded that the RMSH values of the four groups before and after wavelet processing all change more than 200%, the change amplitude reached 271.02% under the treatment of 12 cm ditching depth, meanwhile, the average CL value of five cross-sections under each group of ditching depths decreased by 1.43–2.28 times, which proves that the oriented roughness component formed by furrows and ridges has a significant influence on the calculation of RR. By further analyzing the roughness value differences of clods and pits in different directions and local areas before and after wavelet transform, it was shown that the wavelet transform can effectively remove the surface anisotropy characteristics formed in the tillage direction and provide a uniform treatment method for the evaluation of surface RR at different ditching depths

Evaluation Method of Soil Surface Roughness after Ditching Operation Based on Wavelet Transform

Soil surface roughness (SSR) is an important parameter affecting surface hydrology, erosion, gas exchange and other processes. The surface roughness of the farmland environment is directly related to the tillage process. In order to accurately characterize the random roughness (RR) parameters of the surface after ditching, a three-dimensional (3D) digital model of the surface was obtained by laser scanning under the conditions of an indoor ditching test, and the influence of oriented roughness components formed by removing ridge characteristics on the RR of the surface was analyzed by introducing the wavelet processing method. For this reason, four groups of ditching depths and two types of surface conditions (whether the surface was agglomerated or not) were designed in this paper. By comparing the root mean squared height (RMSH) and correlation length (CL) data calculated before and after wavelet processing under each group of tests, it was concluded that the RMSH values of the four groups before and after wavelet processing all change more than 200%, the change amplitude reached 271.02% under the treatment of 12 cm ditching depth, meanwhile, the average CL value of five cross-sections under each group of ditching depths decreased by 1.43–2.28 times, which proves that the oriented roughness component formed by furrows and ridges has a significant influence on the calculation of RR. By further analyzing the roughness value differences of clods and pits in different directions and local areas before and after wavelet transform, it was shown that the wavelet transform can effectively remove the surface anisotropy characteristics formed in the tillage direction and provide a uniform treatment method for the evaluation of surface RR at different ditching depths

Investigating the Global Performance of the BDS-2 and BDS-3 Joint Real-Time Undifferenced and Uncombined Precise Point Positioning Using RTS Products from Different Analysis Centers

Compared to the traditional ionospheric-free (IF) precise point positioning (PPP) model, the undifferenced and uncombined (UU) PPP has the advantages of lower observation noise and the ability to obtain ionospheric information. Thanks to the IGS (International GNSS Service), real-time service (RTS) can provide RT vertical total electron content (VTEC) products, and an enhanced RT UU-PPP based on the RT-VTEC constraints can be achieved. The global performance of the BeiDou Navigation Satellite System-2 (BDS-2) and BDS-3 joint RT UU-PPP using different RTS products was investigated. There is not much difference in the RTS orbit accuracy of medium earth orbit (MEO) satellites among all analysis centers (ACs), and the optimal orbit accuracy is better than 5, 9, and 7 cm in the radial, along-track, and cross-track directions, respectively. The orbit accuracy of inclined geosynchronous orbit (IGSO) satellites is worse than that of MEO satellites. Except for CAS of 0.46 ns, the RTS clock accuracy of MEO satellites for other ACs achieves 0.2–0.27 ns, and the corresponding accuracy is about 0.4 ns for IGSO satellites. In static positioning, due to the limited accuracy of RT-VTEC, the convergence time of the enhanced RT UU-PPP is longer than that of RT IF-PPP for most ACs and can be better than 25 and 20 min in the horizontal and vertical components, respectively. After convergence, the 3D positioning accuracy of the static RT UU-PPP is improved by no more than 8.7%, and the optimal horizontal and vertical positioning accuracy reaches 3.5 and 7.0 cm, respectively. As for the kinematic mode with poor convergence performance, with the introduction of RT-VTEC constraints, the convergence time of RT UU-PPP can be slightly shorter and reaches about 55 and 60 min in the horizontal and vertical components, respectively. Both the horizontal and vertical positioning accuracies of the kinematic RT UU-PPP can be improved and achieve around 7.5 and 10 cm, respectively

Establish a novel tumor budding-related signature to predict prognosis and guide clinical therapy in colorectal cancer

Abstract Tumor budding is a long-established independent adverse prognostic marker for colorectal cancer (CRC), yet assessment of tumor budding was not reproducible. Therefore, development of precise diagnostic approaches to tumor budding is in demand. In this study, we first performed bioinformatic analysis in our single-center CRC patients’ cohort (n = 84) and identified tumor budding-associated hub genes using the weighted gene co-expression network analysis (WGCNA). A machine learning methodology was used to identify hub genes and construct a prognostic signature. Nomogram model was used to identified hub genes score for tumor budding, and the receiver operating characteristic (ROC) curve and calibration plot indicated high accuracy and stability of hub gene score for predicted the prognosis of CRC. The association between budding-associated hub genes and score and prognosis of CRC were further verified in TCGA CRC cohort (n = 342). Then gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) were applied to explore the signaling pathways related to the tumor budding and validated by immunohistochemistry (IHC) of our clinical samples. Subsequently, immune infiltration analysis demonstrated that there was a high correlation between hub genes score and M2-like macrophages infiltrated in tumor tissue. In addition, somatic mutation and chemotherapeutic response prediction were analyzed based on the risk signature. In summary, we established a tumor budding diagnostic molecular model, which can improve tumor budding assessment and provides a promising novel molecular marker for immunotherapy and prognosis of CRC

Removal of Fouling from Steel Plate Surfaces Based on Multi-Frequency Eco-Friendly Ultrasonic Guided Wave Technology

Fouling is inevitable on the surfaces of industrial equipment, especially on heat-exchanging surfaces in contact with fluids, which causes water pollution and destroys the ecological environment. In this paper, a novel fouling-removal methodology for plate structure based on cavitation by multi-frequency ultrasonic guided waves is proposed, which can remove fouling on stainless steel plates. A numerical simulation method has been developed to study the acoustic pressure distribution on a steel plate. According to the simulation results, the distribution of sound pressure on the plate under triple-frequency excitation is denser and more prone to cavitation than in single-frequency cases and dual-frequency cases, which improves fouling removal rate. The stainless steel plate is immersed in water for the descaling experiment, and the results show that the fouling removal rates of three water-loaded stainless steel plates under different single-frequency excitation seem unsatisfactory. However, the multi-frequency excitation improves the descaling performance and the removal rate of fouling reaches 80%. This new method can be applied to the surface descaling of large equipment plates, which is of great significance for purifying water quality and protecting the ecological environment

Long Non-coding RNA PVT1 Promotes Cell Proliferation and Migration by Silencing ANGPTL4 Expression in Cholangiocarcinoma

Cholangiocarcinoma (CCA) is the most common biliary tract malignancy, with a low survival rate and limited treatment options. Long non-coding RNAs (lncRNAs) have recently been verified to have significant regulatory functions in many kinds of human cancers. It was discovered in this study that the lncRNA PVT1, whose expression is significantly elevated in CCA, could be a molecular marker of CCA. Experiments indicated that PVT1 knockdown greatly inhibited cell migration and proliferation in vitro and in vivo. According to RNA sequencing (RNA-seq) analysis, PVT1 knockdown dramatically influenced target genes associated with cell angiogenesis, cell proliferation, and the apoptotic process. RNA immunoprecipitation (RIP) analysis demonstrated that, by binding to epigenetic modification complexes (PRC2), PVT1 could adjust the histone methylation of the promoter of ANGPTL4 (angiopoietin-like 4) and, thus, promote cell growth, migration, and apoptosis progression. The data verified the significant functions of PVT1 in CCA oncogenesis, and they suggested that PVT1 could be a target for CCA intervention. Keywords: cholangiocarcinoma, lncRNA PVT1, ANGPTL