Does the pain sensitivity questionnaire correlate with tourniquet pain in patients undergoing ankle surgery?

BackgroundTourniquet pain is the most prominent problem in ankle surgery, and there is no proper method to predict it. It was reported that pain sensitivity questionnaires could evaluate the pain sensitivity of subjects. Its potential to predict tourniquet pain in ankle surgery is constructive and meaningful.MethodsOne hundred and twenty patients undergoing ankle surgery were included in this study. The pain sensitivity questionnaire (PSQ) and self-rating anxiety scale (SAS) were completed before the operation. The methods included an ultrasound-guided popliteal sciatic, a femoral nerve block, and a proximal thigh tourniquet. The pressure of the tourniquet was set according to the systolic blood pressure (SBP + 100 mmHg). A visual analogue scale (VAS) was used to assess the tourniquet pain. Also, the onset time of tourniquet pain ≥4 VAS units was recorded.ResultsThe PSQ-total and PSQ-minor scores were significantly correlated with the onset time when the tourniquet pain ≥4 VAS units (r = −0.763, r = −0.731, P < 0.001). The PSQ-total score <6.5 group gave significantly lower ratings for items 3, 4, 14, and 16 in the PSQ survey compared to the PSQ-total score ≥6.5 group (P < 0.05). Patients with high pain sensitivity have a higher need for analgesic drugs (P < 0.001). PSQ-total score ≥6.5 (OR = 185.8, 95% CI = 39.8–1,437.6, P < 0.001), sex (male, OR = 0.11, 95% CI = 0.018–0.488, P < 0.05), and age (OR = 0.92, 95% CI = 0.842–0.995, P < 0.05) were risk factors for reporting a tourniquet pain ≥4 VAS units within 30 min.ConclusionThe PSQ score is found to be correlated with intraoperative tourniquet pain. In addition, sex and age also affect the time of having intraoperative tourniquet pain

Spin State Disproportionation in Insulating Ferromagnetic LaCoO3 Epitaxial Thin Films

The origin of insulating ferromagnetism in epitaxial LaCoO3 films under tensile strain remains elusive despite extensive research efforts have been devoted. Surprisingly, the spin state of its Co ions, the main parameter of its ferromagnetism, is still to be determined. Here, we have systematically investigated the spin state in epitaxial LaCoO3 thin films to clarify the mechanism of strain induced ferromagnetism using element-specific x-ray absorption spectroscopy and dichroism. Combining with the configuration interaction cluster calculations, we unambiguously demonstrate that Co3+ in LaCoO3 films under compressive strain (on LaAlO3 substrate) are practically a low spin state, whereas Co3+ in LaCoO3 films under tensile strain (on SrTiO3 substrate) have mixed high spin and low spin states with a ratio close to 1:3. From the identification of this spin state ratio, we infer that the dark strips observed by high-resolution scanning transmission electron microscopy indicate the position of Co3+ high spin state, i.e., an observation of a spin state disproportionation in tensile-strained LaCoO3 films. This consequently explains the nature of ferromagnetism in LaCoO3 films

Exercise ameliorates the FGF21–adiponectin axis impairment in diet-induced obese mice

Objective: The protective effects of exercise against glucose dysmetabolism have been generally reported. However, the mechanism by which exercise improves glucose homeostasis remains poorly understood. The FGF21–adiponectin axis participates in the regulation of glucose metabolism. Elevated levels of FGF21 and decreased levels of adiponectin in obesity indicate FGF21–adiponectin axis dysfunction. Hence, we investigated whether exercise could improve the FGF21–adiponectin axis impairment and ameliorate disturbed glucose metabolism in diet-induced obese mice. Methods: Eight-week-old C57BL/6J mice were randomly assigned to three groups: low-fat diet control group, high-fat diet group and high-fat diet plus exercise group. Glucose metabolic parameters, the ability of FGF21 to induce adiponectin, FGF21 receptors and co-receptor levels and adipose tissue inflammation were evaluated after 12 weeks of intervention. Results: Exercise training led to reduced levels of fasting blood glucose and insulin, improved glucose tolerance and better insulin sensitivity in high-fat diet-induced obese mice. Although serum FGF21 levels were not significantly changed, both total and high-molecular-weight adiponectin concentrations were markedly enhanced by exercise. Importantly, exercise protected against high-fat diet-induced impaired ability of FGF21 to stimulate adiponectin secretion. FGF21 co-receptor, β-klotho, as well as receptors, FGFR1 and FGFR2, were upregulated by exercise. We also found that exercise inhibited adipose tissue inflammation, which may contribute to the improvement in the FGF21–adiponectin axis impairment. Conclusions: Our data indicate exercise protects against high-fat diet-induced FGF21–adiponectin axis impairment, and may thereby exert beneficial effects on glucose metabolism

Shadow Enhancement Using 2D Dynamic Stochastic Resonance for Hyperspectral Image Classification

With the development of remote sensing technology, classification has become a meaningful way to explore the rich information in hyperspectral images (HSIs). However, various environmental factors may cause noise and shadow areas in HSIs, resulting in weak signals and difficulties in fully utilizing information. In addition, classification methods based on deep learning have made considerable progress, but features extracted from most networks have much redundancy. Therefore, a method based on two-dimensional dynamic stochastic resonance (2D DSR) shadow enhancement and convolutional neural network (CNN) classification combined with an attention mechanism (AM) for HSIs is proposed in this paper. Firstly, to protect the spatial correlation of HSIs, an iterative equation of 2D DSR based on the pixel neighborhood relationship was derived, which made it possible to perform matrix SR in the spatial dimension of the image, instead of one-dimensional vector resonance. Secondly, by using the noise in the shadow area to generate resonance, 2D DSR can help increase the signals in the shadow regions by preserving the spatial characteristics, and enhanced HSIs can be obtained. Then, a 3DCNN embedded with two efficient channel attention (ECA) modules and one convolutional block attention module (CBAM) was designed to make the most of critical features that significantly affect the classification accuracy by giving different weights. Finally, the performance of the proposed method was evaluated on a real-world HSI, and comparative studies were carried out. The experimental results showed that the proposed approach has promising prospects in HSIs’ shadow enhancement and information mining

Comprehensive Genome-Wide Analysis of Histone Acetylation Genes in Roses and Expression Analyses in Response to Heat Stress

International audienceRoses have high economic values as garden plants and for cut-flower and cosmetics industries. The growth and development of rose plants is affected by exposure to high temperature. Histone acetylation plays an important role in plant development and responses to various stresses. It is a dynamic and reversible process mediated by histone deacetylases (HDAC) and histone acetyltransferases (HAT). However, information on HDAC and HAT genes of roses is scarce. Here, 23 HDAC genes and 10 HAT genes were identified in the Rosa chinensis ‘Old Blush’ genome. Their gene structures, conserved motifs, physicochemical properties, phylogeny, and synteny were assessed. Analyses of the expression of HDAC and HAT genes using available RNAseq data showed that these genes exhibit different expression patterns in different organs of the three analyzed rose cultivars. After heat stress, while the expression of most HDAC genes tend to be down-regulated, that of HAT genes was up-regulated when rose plants were grown at high-temperature conditions. These data suggest that rose likely respond to high-temperature exposure via modification in histone acetylation, and, thus, paves the way to more studies in order to elucidate in roses the molecular mechanisms underlying rose plants development and flowering

Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity

Abstract Background Biological nitrogen fixation converting atmospheric dinitrogen to ammonia is an important way to provide nitrogen for plants. Pseudomonas stutzeri DSM4166 is a diazotrophic Gram-negative bacterium isolated from the rhizosphere of cereal Sorghum nutans. Endogenous constitutive promoters are important for engineering of the nitrogen fixation pathway, however, they have not been systematically characterized in DSM4166. Results Twenty-six candidate promoters were identified from DSM4166 by RNA-seq analysis. These 26 promoters were cloned and characterized using the firefly luciferase gene. The strengths of nineteen promoters varied from 100 to 959% of the strength of the gentamicin resistance gene promoter. The strongest P12445 promoter was used to overexpress the biological nitrogen fixation pathway-specific positive regulator gene nifA. The transcription level of nitrogen fixation genes in DSM4166 were significantly increased and the nitrogenase activity was enhanced by 4.1 folds determined by the acetylene reduction method. The nifA overexpressed strain produced 359.1 µM of extracellular ammonium which was 25.6 times higher than that produced by the wild-type strain. Conclusions The endogenous strong constitutive promoters identified in this study will facilitate development of DSM4166 as a microbial cell factory for nitrogen fixation and production of other useful compounds

High Mechanical Performance Based on Physically Linked Double Network (DN) Hydrogels

A new design strategy was proposed to improve the mechanical performance of double network (DN) hydrogels by introducing polyhydroxy compounds into the DN structure and form a physically linked double network through the interaction of hydrogen bonding. Herein, agar/poly(acrylic acid)/hydroxyethyl cellulose composite hydrogels could be prepared by a simple one-pot method. The resulting hydrogels exhibit highly mechanical properties and excellent recoverability, which have potential applications in biomedical fields

3D printing of bone scaffolds for treating infected mandible bone defects through adjustable dual-release of chlorhexidine and osteogenic peptide

Preventing implant-related bone infection during treating mandibular bone defect is still challenging as intractable bacterial infection in the irregular defects often leads to surgical failure and other fatal complications. Herein, we fabricate a multifunctional scaffold with controlled dual-stage drug release to achieve antibacterial and osteogenic therapy during infected bone reconstruction. Water-in-oil (w/o) β-tricalcium phosphate (TCP) nanoparticle/ poly(Lactic-co-glycolic acid) (PLGA)/dichloromethane composite emulsion inks containing the antibacterial chlorhexidine (CHX) loaded graphene oxide (GO) nanosheets and osteogenic peptide (p24) was produced through cryo-3D printing. A biphasic drug release profile is achieved via a rapid CHX release from CHX@GO on scaffold surface to kill the bacteria, and a sustained release of CHX and p24 from the scaffold matrix to prevent the recurrence of infection and induce bone regeneration. Interestingly, the GO nanosheet increased the antibacterial sensitivity through its direct physical contact which destroyed the bacteria membrane. Furthermore, the p24 and phosphate ions derived from dissociated TCP nanoparticles could induce the osteogenic differentiation of bone marrow derived stem cells. Eventually, the designed scaffolds effectively eliminated the bacteria, alleviated the accompanying inflammation, and improved bone regeneration in a rat model. The present study provides a facile strategy to treat bone defects with infection