Identification of a novel prognostic and therapeutic prediction model in clear cell renal carcinoma based on Renin-angiotensin system related genes

BackgroundClear cell renal cell carcinoma is the most predominant type of renal malignancies, characterized by high aggressiveness and probability of distant metastasis. Renin angiotensin system (RAS) plays a crucial role in maintaining fluid balance within the human body, and its involvement in tumorigenesis is increasingly being uncovered, while its role in ccRCC remains unclear.MethodsWGCNA was used to identify RAS related genes. Machine learning was applied to screen hub genes for constructing risk model, E-MTAB-1980 dataset was used for external validation. Transwell and CCK8 assays were used to investigate the impact of SLC6A19 to ccRCC cells.ResultsSLC6A19, SLC16A12 and SMIM24 were eventually screened to construct risk model and the predictive efficiency for prognosis was validated by internal and external cohorts. Moreover, the differences were found in pathway enrichment, immune cell infiltration, mutational landscapes and drug prediction between high and low risk groups. Experimental results indicated that SLC6A19 could inhibit invasion and proliferation of ccRCC cells and GSEA pinpointed that SLC6A19 was intimately correlated with fatty acid metabolism and CPT1A.ConclusionThe risk model based on the three RAS-related genes have a robust ability to predict the prognosis and drug sensitivity of ccRCC patients, further providing a valid instruction for clinical care

Mechanical Properties and Fracture Analysis of High Strength Bolts in Nuclear Power Plant

Abstract A high-strength bolt of a steel structure of a nuclear power plant failed and fell off. In order to determine the cause of the bolt’s fracture, a series of tests such as macro inspection, physical and chemical performance test, and microscopic analysis of the fracture were carried out on the failed bolt. The results show that the cause of bolt fracture is hydrogen embrittlement. Hydrogen infiltrated into the bolt during the phosphating process and the corrosion process during use, and then hydrogen continued to accumulate eventually causing hydrogen embrittlement fracture. Dehydrogenation treatment should be performed immediately after the phosphorylation treatment, and the anti-corrosion method of the bolt should be optimized, which is an effective measure to prevent the hydrogen embrittlement of high strength bolts.</jats:p

A method for determining the optimum supporting opportunity of roadway based on energy dissipation.

The time and space of the support structure applying is related to the overall stability of the roadway after excavation directly. Designed twenty-four groups of roadway support schemes with time and space dimensions, and studied the stability characteristics of roadway in different schemes by using Fast Lagrangian Analysis of Continua in Three Dimensions. The main conclusions are as follows: the influence of energy dissipation (time) and support position (space) on the stability of the roadway is not a linear relationship, and supporting at the appropriate opportunity can be beneficial. Established the "displacement-dissipation energy" curve, founding that there is an obvious "jump" phenomenon in the dissipation energy of surrounding rock during the process of gradually increasing displacement. A novel method for determining the optimum supporting opportunity of roadway based on energy dissipation was proposed, based on the above finding. This study can provide an original idea for the determination of roadway supporting opportunity