13 research outputs found

    Ecological Zoning Management Strategies in China: A Perspective of Ecosystem Services Supply and Demand

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    Ecological zoning management aims to ensure ecological functions and improve environmental quality, serving as an essential means to optimize the development and protection of territorial space. However, comprehensive research on ecological zoning management that combines human needs with natural resources is still relatively scarce. In this study, we selected water yield (WY), food provision (FP), and carbon sequestration (CS) as the critical ecosystem services (ES) in China. An InVEST model, ecosystem services supply–demand index (ESI), random forest (RF), and geographically and temporally weighted regression (GTWR) were used to analyze the spatiotemporal characteristics and influencing factors of ES supply and demand, and the four-quadrant model was used to analyze the spatial matching patterns. The results showed that: (1) from 2005 to 2020, the supply and demand of WY, FP, and CS increased. Among them, WY, FP, and CS supply increased by 16.06%, 34%, and 22.53%, respectively, while demand increased by 5.63%, 12.4%, and 83.02%, respectively; (2) the supply of WY and CS follow a “high in the southeast and low in the northwest” pattern, while all of the demands exhibit a “high in the east and low in the west” pattern; and (3) the average ecosystem service supply–demand index (ESI) values for WY, FP, and CS in China are 0.45, 0.12, and −0.24, respectively, showing an overall upward trend. The study identified three dominant functional zones for WY, FP, and CS, and four classification management zones, including protection zones, conservation zones, improvement zones, and reconstruction zones. These research findings provide a scientific basis for future territorial space planning in China and the application of ecosystem service supply and demand in sustainable development

    Human interferon-ß inhibits binding of HIV-1 gp41 to lymphocyte and monocyte cells and binds the potential receptor protein P50 for HIV-1 gp41

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    Previous findings have indicated that HIV-1 gp41 like human type I interferon (IFN) could inhibit lymphocyte proliferation and up-modulate MHC class I, II and ICAM-1 molecule expression, and a common epitope exists between gp41 and type I interferon (IFN-α and -ß) in the receptor binding regions. To clarify the relationship between human type I interferon and HIV- 1 gp41, we tried to inhibit recombinant soluble gp41-binding to human T, B and monocyte cell lines by human IFN-α, -ß and -y. It was interestingly observed that IFN-ß after preincubating with cells could inhibit the binding of rsgp41 to H9, Raji and U937 cells (T, B and monocyte cell lines), while this binding could not be inhibited by another type I interferon (IFN-α) and a type II interferon (IFN-ß). It was further examined whether human IFN-α and -ß bind to the gp41 binding protein P50. In ELISA-assay, the human IFN- ß, but not IFN-α, could bind to P50 which was identified as a potential cellular receptor protein for gp41-binding. By the affinity capillary electrophoresis (ACE) analysis, formation of stable IFN-ß-P50 complex was observed. These results indicate that IFN-ß binds the potential receptor protein P50. Based on these experimental evidences and previous studies, it was presumed that the potential cellular receptor protein P50 may be the 51 kDa subunit of human IFN-α/ß receptor, which needs to be verified in the future

    Characterization of ginsenosides from Panax japonicus var. major (Zhu-Zi-Shen) based on ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry and desorption electrospray ionization-mass spectrometry imaging

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    Abstract Background Panax japonicus var. major (PJM) belongs to the well-known ginseng species used in west China for hundreds of years, which has the effects of lung tonifying and yin nourishing, and exerts the analgesic, antitussive, and hemostatic activities. Compared with the other Panax species, the chemical composition and the spatial tissue distribution of the bioactive ginsenosides in PJM have seldom been investigated. Methods Ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/QTOF-MS) and desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) were integrated for the systematic characterization and spatial tissue distribution studies of ginsenosides in the rhizome of PJM. Considering the great difficulty in exposing the minor saponins, apart from the conventional Auto MS/MS (M1), two different precursor ions list-including data-dependent acquisition (PIL-DDA) approaches, involving the direct input of an in-house library containing 579 known ginsenosides (M2) and the inclusion of the target precursors screened from the MS1 data by mass defect filtering (M3), were developed. The in situ spatial distribution of various ginsenosides in PJM was profiled based on DESI-MSI with a mass range of m/z 100–1500 in the negative ion mode, with the imaging data processed by the High Definition Imaging (HDI) software. Results Under the optimized condition, 272 ginsenosides were identified or tentatively characterized, and 138 thereof were possibly not ever reported from the Panax genus. They were composed by 75 oleanolic acid type, 22 protopanaxadiol type, 52 protopanaxatriol type, 16 octillol type, 19 malonylated, 35 C-17 side-chain varied, and 53 others. In addition, the DESI-MSI experiment unveiled the differentiated distribution of saponins, but the main location in the cork layer and phloem of the rhizome. The abundance of the oleanolic acid ginsenosides was high in the rhizome slice of PJM, which was consistent with the results obtained by UHPLC/QTOF-MS. Conclusion Comprehensive characterization of the ginsenosides in the rhizome of PJM was achieved, with a large amount of unknown structures unveiled primarily. We, for the first time, reported the spatial tissue distribution of different subtypes of ginsenosides in the rhizome slice of PJM. These results can benefit the quality control and further development of PJM and the other ginseng species
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