337 research outputs found

    Models of Relative Nearness Incidence Based on Standard Distance Entropy

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    The model of grey nearness incidence cannot reflect the nearness degree of sequences correctly. Therefore, the model of relative nearness incidence of sequences, curves, and surfaces is suggested based on standard distance entropy to remove the current bottlenecks and its properties are studied. At last, three cases are exemplified to demonstrate the validity and practicability of relative nearness incidence. The proposed models have enriched the theory of grey nearness incidence, filled the defects of grey nearness incidence

    Fuzzy Adaptive Shift Schedule of Tractor Subjected to Random Load

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    In this paper, the low frequency random load of a tractor is presented. Controlled by a theoretical three-parameter shift schedule, the random load would frequently trigger the random shift. Simultaneously, the driving force of the tractor should be consistent prior to and following this shift. Additionally, the higher traction efficiency and improved load utilization rate should be ensured by a choice of a transmission ratio of the tractor power shift transmission. The shift schedule was utilized for the aforementioned problems solution. An innovative method is presented for theoretical shift schedule modification by the fuzzy algorithm, based on the random load standard deviation and the alteration rate of both steady state values of the load and of the throttle position. The simulation results demonstrated that the modified shift schedule could discover the running state of the tractor. By shielding the random shift judgment caused by the random load, the stability of the tractor was ensured. When the shift was required, the schedule could rapidly respond, whereas the tractor driving force did not sustain a sudden alteration. The schedule could also automatically select and maintain the transmission ratio with higher traction efficiency

    Mobile E-business Platform: Collaboration System of Wi-Fi and WAVE Based on Cognitive Radio

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    The new international standard IEEE802.11p (WAVE) defined enhancements to IEEE802.11 (Wi-Fi). A new mobile e-business platform of Wi-Fi and WAVE is described. Based on cognitive radio, the popular wireless network Wi-Fi can collaborate with WAVE in their common unlicensed spectrum band and licensed spectrum band. As a core technology in the cognitive radio field, OFDM is introduced, and a novel frequency estimation algorithm for them is presented

    Highly Efficient Optical Beam Steering Using an In-Fiber Diffraction Grating for Full Duplex Indoor Optical Wireless Communication

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    Diffraction gratings have been widely used in wavelength-controlled non-mechanical laser beam steering for high data-rate indoor optical wireless communications (OWC). Existing free-space diffraction gratings suffer from inherent difficulties of limited diffraction efficiency, bulky configuration, high cost and significant coupling loss with optical fiber links. In this work, a new optical approach for highly efficient, compact and fiber compatible laser beam steering using an in-fiber diffraction grating is proposed and experimentally demonstrated for the first time to our best knowledge. In-fiber diffraction is made possible based on a 45° tilted fiber grating (TFG), where wavelength dependent lateral scattering is obtained due to the strongly tilted grating structure. Improved diffraction efficiency of 93.5% has been achieved. In addition, the 45° TFG works perfectly for both light emission and reception, enabling full-duplex optical wireless transmission. Utility of the 45° TFG in all-fiber laser beam steering for multi-user full duplex optical wireless communications has been verified in experiments. 1.4 m free-space full-duplex wireless transmission has been demonstrated with data rate up to 12 Gb/s per beam using 2.4 GHz bandwidth OFDM signals

    cDNA-AFLP analysis reveals differential gene expression in compatible interaction of wheat challenged with Puccinia striiformis f. sp. tritici

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    <p>Abstract</p> <p>Background</p> <p><it>Puccinia striiformis </it>f. sp. <it>tritici </it>is a fungal pathogen causing stripe rust, one of the most important wheat diseases worldwide. The fungus is strictly biotrophic and thus, completely dependent on living host cells for its reproduction, which makes it difficult to study genes of the pathogen. In spite of its economic importance, little is known about the molecular basis of compatible interaction between the pathogen and wheat host. In this study, we identified wheat and <it>P. striiformis </it>genes associated with the infection process by conducting a large-scale transcriptomic analysis using cDNA-AFLP.</p> <p>Results</p> <p>Of the total 54,912 transcript derived fragments (TDFs) obtained using cDNA-AFLP with 64 primer pairs, 2,306 (4.2%) displayed altered expression patterns after inoculation, of which 966 showed up-regulated and 1,340 down-regulated. 186 TDFs produced reliable sequences after sequencing of 208 TDFs selected, of which 74 (40%) had known functions through BLAST searching the GenBank database. Majority of the latter group had predicted gene products involved in energy (13%), signal transduction (5.4%), disease/defence (5.9%) and metabolism (5% of the sequenced TDFs). BLAST searching of the wheat stem rust fungus genome database identified 18 TDFs possibly from the stripe rust pathogen, of which 9 were validated of the pathogen origin using PCR-based assays followed by sequencing confirmation. Of the 186 reliable TDFs, 29 homologous to genes known to play a role in disease/defense, signal transduction or uncharacterized genes were further selected for validation of cDNA-AFLP expression patterns using qRT-PCR analyses. Results confirmed the altered expression patterns of 28 (96.5%) genes revealed by the cDNA-AFLP technique.</p> <p>Conclusion</p> <p>The results show that cDNA-AFLP is a reliable technique for studying expression patterns of genes involved in the wheat-stripe rust interactions. Genes involved in compatible interactions between wheat and the stripe rust pathogen were identified and their expression patterns were determined. The present study should be helpful in elucidating the molecular basis of the infection process, and identifying genes that can be targeted for inhibiting the growth and reproduction of the pathogen. Moreover, this study can also be used to elucidate the defence responses of the genes that were of plant origin.</p

    Human papillomavirus and cervical cancer in the microbial world: exploring the vaginal microecology

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    The vaginal microbiota plays a crucial role in female reproductive health and is considered a biomarker for predicting disease outcomes and personalized testing. However, its relationship with human papillomavirus (HPV) infection and cervical cancer is not yet clear. Therefore, this article provides a review of the association between the vaginal microbiota, HPV infection, and cervical cancer. We discuss the composition of the vaginal microbiota, its dysbiosis, and its relationship with HPV infection, as well as potential mechanisms in the development of cervical cancer. In addition, we assess the feasibility of treatment strategies such as probiotics and vaginal microbiota transplantation to modulate the vaginal microbiota for the prevention and treatment of diseases related to HPV infection and cervical cancer. In the future, extensive replication studies are still needed to gain a deeper understanding of the complex relationship between the vaginal microbiota, HPV infection, and cervical cancer, and to clarify the role of the vaginal microbiota as a potential biomarker for predicting disease outcomes, thus providing a theoretical basis for personalized testing

    The Effect of the Antimicrobial Peptide Plectasin on the Growth Performance, Intestinal Health, and Immune Function of Yellow-Feathered Chickens

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    The goal of the study was to test the effects of an antibiotic substitute, plectasin, on the growth performance, immune function, intestinal morphology and structure, intestinal microflora, ileal mucosal layer construction and tight junctions, ileal immune-related cytokines, and blood biochemical indices of yellow-feathered chickens. A total of 1,500 one-day-old yellow-feathered chicks were randomly divided into four dietary treatment groups with five replicates in each group and 75 yellow-feathered chicks in each replication, as follows: basal diet (group A); basal diet supplemented with 10 mg enramycin/kg of diet (group B), basal diet supplemented with 100 mg plectasin/kg of diet (group C), and basal diet supplemented with 200 mg plectasin/kg of diet (group D). It was found that the dietary antimicrobial peptide plectasin could improve the ADG and had better F/G for the overall period of 1–63 days. Dietary plectasin can enhance H9N2 avian influenza virus (AIV) and Newcastle disease virus (NDV) antibody levels of yellow-feathered chickens at 21, and 35 days of age. Dietary plectasin can enhance the intestine structure, inhibit Escherichia coli and proinflammatory cytokines in the ileum, and ameliorate the blood biochemical indices of yellow-feathered chickens at 21 days of age. This study indicates that the antimicrobial peptide plectasin has beneficial effects on the growth performance, intestinal health and immune function of yellow-feathered chickens

    Conservation and distribution of the DRACH motif for potential m6A sites in avian leukosis virus subgroup J

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    N6-methyladenosine (m6A) methylation is an internal post-transcriptional modification that has been linked to viral multiplication and pathogenicity. To elucidate the conservation patterns of potential 5′-DRACH-3′ motifs in avian leukosis virus subgroup J (ALV-J), 149 ALV-J strains (139 isolates from China; ALV-J prototype HPRS-103 from the UK; and 9 strains from the USA, Russia, India, and Pakistan) available in GenBank before December 2023 were retrieved. According to the prediction results of the SRAMP web-server, these ALV-J genomes contained potential DRACH motifs, with the total number ranging from 43 to 64, which were not determined based on the isolation region and time. Conservative analysis suggested that 37 motifs exhibited a conservation of &gt;80%, including 17 motifs with a grading above “high confidence.” Although these motifs were distributed in the U5 region of LTRs and major coding regions, they were enriched in the coding regions of p27, p68, p32, and gp85. The most common m6A-motif sequence of the DRACH motif in the ALV-J genome was GGACU. The RNA secondary structure of each conserved motif predicted by SRAMP and RNAstructure web-server was mainly of two types—A–U pair (21/37) and hairpin loop (16/37)—based on the core adenosine. Considering the systematic comparative analysis performed in this study, future thorough biochemical research is warranted to determine the role of m6A modification during the replication and infection of ALV-J. These conservation and distribution analysis of the DRACH motif for potential m6A sites in ALV-J would provide a foundation for the future intervention of ALV-J infection and m6A modification
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