Automated Long-Term Monitoring of Parallel Microfluidic Operations Applying a Machine Vision-Assisted Positioning Method

As microfluidics has been applied extensively in many cell and biochemical applications, monitoring the related processes is an important requirement. In this work, we design and fabricate a high-throughput microfluidic device which contains 32 microchambers to perform automated parallel microfluidic operations and monitoring on an automated stage of a microscope. Images are captured at multiple spots on the device during the operations for monitoring samples in microchambers in parallel; yet the device positions may vary at different time points throughout operations as the device moves back and forth on a motorized microscopic stage. Here, we report an image-based positioning strategy to realign the chamber position before every recording of microscopic image. We fabricate alignment marks at defined locations next to the chambers in the microfluidic device as reference positions. We also develop image processing algorithms to recognize the chamber positions in real-time, followed by realigning the chambers to their preset positions in the captured images. We perform experiments to validate and characterize the device functionality and the automated realignment operation. Together, this microfluidic realignment strategy can be a platform technology to achieve precise positioning of multiple chambers for general microfluidic applications requiring long-term parallel monitoring of cell and biochemical activities

Expression of Hemolysin Is Regulated Under the Collective Actions of HapR, Fur, and HlyU in Vibrio cholerae El Tor Serogroup O1

The biotype El Tor of serogroup O1 and most of the non-O1/non-O139 strains of Vibrio cholerae can produce an extracellular pore-forming toxin known as cholera hemolysin (HlyA). Expression of HlyA has been previously reported to be regulated by the quorum sensing (QS) and the regulatory proteins HlyU and Fur, but lacks the direct evidence for their binding to the promoter of hlyA. In the present work, we showed that the QS regulator HapR, along with Fur and HlyU, regulates the transcription of hlyA in V. cholerae El Tor biotype. At the late mid-logarithmic growth phase, HapR binds to the three promoters of fur, hlyU, and hlyA to repress their transcription. At the early mid-logarithmic growth phase, Fur binds to the promoters of hlyU and hlyA to repress their transcription; meanwhile, HlyU binds to the promoter of hlyA to activate its transcription, but it manifests direct inhibition of its own gene. The highest transcriptional level of hlyA occurs at an OD600 value of around 0.6–0.7, which may be due to the subtle regulation of HapR, Fur, and HlyU. The complex regulation of HapR, Fur, and HlyU on hlyA would be beneficial to the invasion and pathogenesis of V. cholerae during the different infection stages

Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats

Background: Continuous damage from oxidative stress and apoptosis are the important mechanisms that facilitate chronic heart failure (CHF). Molecular hydrogen (H2) has potentiality in the aspects of anti-oxidation. The objectives of this study were to investigate the possible mechanism of H2 inhalation in delaying the progress of CHF.Methods and Results: A total of 60 Sprague-Dawley (SD) rats were randomly divided into four groups: Sham, Sham treated with H2, CHF and CHF treated with H2. Rats from CHF and CHF treated with H2 groups were injected isoprenaline subcutaneously to establish the rat CHF model. One month later, the rat with CHF was identified by the echocardiography. After inhalation of H2, cardiac function was improved vs. CHF (p < 0.05), whereas oxidative stress damage and apoptosis were significantly attenuated (p < 0.05). In this study, the mild oxidative stress was induced in primary cardiomyocytes of rats, and H2 treatments significantly reduced oxidative stress damage and apoptosis in cardiomyocytes (p < 0.05 or p < 0.01). Finally, as a pivotal transcription factor in reactive oxygen species (ROS)-apoptosis signaling pathway, the expression and phosphorylation of p53 were significantly reduced by H2 treatment in this rat model and H9c2 cells (p < 0.05 or p < 0.01).Conclusion: As a safe antioxidant, molecular hydrogen mitigates the progression of CHF via inhibiting apoptosis modulated by p53. Therefore, from the translational point of view and speculation, H2 is equipped with potential therapeutic application as a novel antioxidant in protecting CHF in the future

Ownership Structure and Stock Price Crash Risk: Evidence from China

This paper examines how corporate ownership concentration affects stock price crash risk in Chinese listed firms. Results show that ownership concentration is negatively associated with firm-specific crash risk and this negative relation is robust against difference-in-difference test. Further evidence demonstrates that the negative relation between ownership concentration and stock price crash risk is more pronounced in privately held firms than in state-owned firms

Responses of Baseflow to Ecological Construction and Climate Change in Different Geomorphological Types in The Middle Yellow River, China

Baseflow is a critical component of streamflow in arid areas. Determining variations in baseflow and the factors affecting it have positive roles for water resource utilization in arid watersheds. Two watersheds, the Hailiutu River watershed (HLTR) and the Dali River watershed (DLR), located in two different geomorphological regions of the middle Yellow River, were selected for this study. By using the Eckhardt segment method (fourth digital filtering method, DF4), baseflow was separated from streamflow based on its daily data. Mann-Kendall trend test analysis (M-K trend test) was used to test the trends in baseflow change at different times. Complex Morlet wavelet analysis was used to judge baseflow periodicity. Heuristic segmentation and sequential cluster analysis were used to identify the potential change points in the baseflow series for the two regions together with Pearson correlation coefficient. The results showed: (1) the annual baseflow of the HLTR and the DLR showed significantly decreasing trends (P < 0.01), more significantly for the HLTR (0.33 × 108 m3) than the DLR (0.20 × 108 m3). The annual base flow index (BFI, baseflow/total streamflow) in the wind-sand region (0.75) was larger than for the loess region (0.55), and the BFI in the wind-sand region was more stable in different periods. (2) The annual baseflow of the HLTR and the DLR both exhibited a complete main cycle of 42 years and 38 years, respectively. The change points of the annual baseflow in the HLTR were 1967 and 1986, and 1971 and 1996 in the DLR. (3) There was no significant change in annual precipitation in the two watersheds, while annual reference evapotranspiration (ET0) in the DLR showed a significant increasing trend (P < 0.01). The normalized difference vegetation index (NDVI) on the DLR (0.40) was higher than on the HLTR (0.26). (4) Baseflow in the wind-sand region, where vegetation improvement was the only ecological activity, decreased faster than in the loess region where there had been numerous ecological measures such as vegetation improvement, check dams and terraces. This implied that comprehensive measures such as these were helpful in slowing the rate at which the baseflow decreases. Therefore, the effect of ecological construction should be considered in future baseflow studies in other geomorphological types within the Middle Yellow River Basin

Evolution of the Relationship between Runoff and Sediment Transport during Flood Event in the Chabagou Watershed of the Loess Plateau

Research on flood events is one of the most important parts to study runoff and sediment transport in the typical watershed on the Loess Plateau. Based on 101 floods in Period I (PD-I, 1970 to 1990) and Period II (PD-II, 2006 to 2018), and combined with rainfall data, the study indicated the evolution of runoff and sediment transport characteristics during flood events in the Chabagou watershed, and reveal its influencing factors in both periods. Results showed: (1) Sediment yield (SY) increased linearly with runoff amount (RA), and the increasing rate of SY in PD-II was around 20% of PD-I, the relationship between peak flow (PF) and sediment concentration (SC) was the power function, and the SC in PD-II was lower than that in PD-I under the same PF. (2) SY was more sensitive to P (precipitation) of the flood event than rainfall intensity (RI), and the sensitivity of RA and SY to P in PD-II was greater than that in PD-I. The sediment delivery modulus (SDM) with rainfall erosivity (RE) was also linear, and the increasing rate of SDM in PD-II was 27% of PD-I. (3) The better improvement of the underlying surface not only raised the threshold of RA and corresponded with peak SC, but also shortened the duration of high sediment concentration and lowered the sediment transportation capacity by contrasting the flood processes

Study of data center communication network topologies using complex network propagation model

Data center, as the core infrastructure of data storage and processing, calls for network security protection. Information security has been addressed in a number of specific ways. However, there are few studies that employ network topology features to prevent the transmission of viruses. When a virus spreads, different topologies display various properties. In this paper, we study three types of data center network topologies, i.e., Fat-tree, Leaf-spine, and Bcube, and quantify the propagation characteristics in every topology through the IC propagation model. The probability of the device being infected, the count of propagation sources, the access of propagation sources, and the topological parameters are all considered. Given that network security defenders can only change the topology and topological parameters, we propose a computational framework that combines factor analysis, which provides us with the selection of network topological parameters with a low virus propagation rate in the candidate parameter set. Through experiments, we find that Leaf-spine has a good inhibitory effect on viruses with high propagation probability. Meanwhile, each offers unique advantages. We hope that more data center network topologies will be studied to improve the security of all data centers using these network topologies

Design and Optimization of an S-Band MEMS Bandpass Filter Based on Aggressive Space Mapping

Aggressive space mapping (ASM) is a common filter simulation and debugging method. It plays an important role in the field of microwave device design. This paper introduces ASM and presents the design and fabrication of a compact fifth-order microstrip interdigital filter with a center frequency of 2.5 GHz and a relative bandwidth of 10% using ASM. The filter used a double-layer silicon substrate structure and stepped impedance resonators (SIRs) and was optimized by ASM. After five iterations, the filter achieved the design specification, which greatly improves the efficiency of the filter design compared with the traditional method. It was fabricated on high-resistance silicon wafers by micro-electro-mechanical systems (MEMSs) technology, and the final size of the chip is 9.5 mm × 7.6 mm × 0.8 mm. The measurement results show that the characteristics of the filter are similar to the simulation results, which also shows the efficiency and precision of the ASM algorithm

Improved Tolerance of Artemisia ordosica to Drought Stress via Dark Septate Endophyte (DSE) Symbiosis

Dark septate endophytes (DSEs) usually colonize plant roots, especially in stress environments. However, their relationship with plants ranges from beneficial to harmful and has remained largely uncharacterized. In the present study, 14 DSE species grouped into 11 genera were isolated from the roots of a desert plant, Artemisia ordosica, which is widely distributed in northwest China. Three dominant DSE species—Paraphoma chrysanthemicola (Pc), Alternaria chartarum (Ac), and Acrocalymma vagum (Av)—were selected and tested for their resistance to drought in vitro. Furthermore, we characterized the responses of A. ordosica under drought conditions in relation to the presence of these DSEs following inoculation. The results showed that all three strains grew well under in vitro drought stress, and the biomass of Ac and Av was significantly higher than that of the unstressed control. The effects of DSE inoculation on the growth of A. ordosica under drought stress varied according to the different DSE species but were generally beneficial. Under drought stress, Av and Pc promoted plant growth, antioxidant enzyme activity, and root development of the hosts. The Ac strain conferred obvious positive effects on the antioxidant enzyme activity of the hosts. In general, Av and Pc demonstrated better application potential for improving the drought resistance of A. ordosica