Polymorphisms of CYP1A1 I462V and GSTM1 genotypes and lung cancer susceptibility in Mongolian

Aim: To study the genotype of cytochrome P450 1A1(CYP1A1) I462V and glutathions S-transferase M1( GSTM1) and the relationship of the genetic polymorphism of them with the susceptibility of lung cancer in Mongolia of China. 

Methods: Allele-specific PCR and a multiplex PCR were employed to identify the genotypes of I462V of CYP1A1 and GSTM1 in a case-control study of 210 lung cancer patients with bronchoscopy diagnosis and 210 matched controls free of malignancy.

Results: The frequencies of the variant CYP1A1(Val/Val) genotypes and GSTM1(-) in lung cancer groups were higher than that in control groups (15.24% vs 7.4% and 56.67% vs 40.95% ). The individuals who carried with CYP1A1(Val/Val) or GSTM1(-) genotype had a significantly higher risk of lung cancer, the OR is 2.56 and 1.89 respectively. Stratified histologically the relative risk increased to 2.6 - fold when the patients carried with two valine alleles than the ones carried one valine allele in cases of SCC. GSTM1(-) genotype is the risk factor of SCC (OR=2.39) and AC(OR=2.16). The presence of at least one Val allele of CYP1A1 and GSTM1(-), the risk of lung cancer was increased, the OR was 4.15 for one Val allele and GSTM1(-) and 2.67 for two Val alleles and GSTM1 Considering ages and smoking status, the risk of lung cancer increased when the age less than 50 who carried with CYP1A1 valine (one or two) alleles or the age during the 51 to 65 who carried with GSTM1(-) genotype. The light smokers with CYP1A1 valine alleles and GSTM1(-) have a high risk for lung cancer. No association was found between the light and heavy drinkers with the susceptibility of lung cancer and the genetic polymorphisms of CYP1A1 I462V and GSTM1(-). 

Conclusion: The valine allele of CYP1A1 was the risk factors of lung cancer especially for SCC and GSTM1(-) also was the risk factor of lung cancer and especially for SCC and AC of Mongolian, China. Light smoking has a influence each other with genotype of CYP1A1 I462V and GSTM1(-) and susceptibility of lung cancer. No relationship was found between the susceptibility of lung cancer and drinkers with genetic polymorphisms of CYP1A1 I462V and GSTM1(-). The influence of genotypes on the susceptibility of lung cancer may depend on the ages. There may be a synergetic interaction between CYP1A1 valine allele and GSTM1(-) genotypes on the elevated susceptibility of lung cancer. So do those genotypes with light smokers. Key words polymorphism; genotype; lung cancer; cytochrome P450；glutathione S-transferase Abbreviations: SCC, squamous cell carcinoma; AC, adenocarcinoma; SCLC, small cell lung cancer; LCLC, large cell lung cance

Numerical simulation of the magnetic field and electromagnetic vibration analysis of the AC permanent-magnet synchronous motor

The AC permanent-magnet synchronous motor with characteristics of high efficiency, high stability, long using life and simple maintenance is one of the most important developing directions in the motor field. The research on electromagnetic vibration is foundations of designing the low noising AC permanent-magnet synchronous motor. In this paper, starting from the structure of AC permanent-magnet synchronous motor, the magnetic flux density, radial electromagnetic force and vibration’s theoretical model of the three-phase AC permanent-magnet synchronous motor were deduced. Influencing factors of the electromagnetic vibration of permanent-magnet synchronous motor were analyzed from the theoretical point of view. The main frequency of electromagnetic vibration was put forward. To analyze the distribution of the electromagnetic field accurately, the three-phase AC permanent-magnet synchronous motor with 4 poles and 24 slots was simulated by numerical simulation of magnetic field, obtaining the radial flux density. By theoretical modeling and the numerical simulation of the magnetic field, the simulation results showed that when slots of the permanent-magnet synchronous motor was close to 2 times, 4 times, 6 times or 8 times of motor poles, the motor generated vibration frequency which is 2 times, 4 times or 6 times as the fundamental frequency

A comprehensive workflow for real time injection-production optimization based on equilibrium displacement

        Irregular well network with high oil recovery rate is used in the development of offshore oilfield, which usually leads to imbalanced waterflooding and poor development performance. In this paper, according to the Buckley-Leverett Equation and general waterflooding theory, a quantitative relationship between water-cut, liquid production and water injection rate is gained to improve the unbalanced lateral waterflooding of the present well network. All the single-well water-cuts are considered to obtain balanced waterflooding of present well network through liquid production and water injection rate adjustments. A new injection-production adjustment method is proposed, with the corresponding calculation program being compiled to realize real-time optimization and adjustment. This method is applied to the 1-1195-1 sand body of Bohai BZ Oilfield. The daily oil increment is 80 m3/d and the cumulative annual oil increment is 2.6×104 m3 , which is consistent with the expected program. It can therefore contribute to engineers’ optimizing the injection-production strategy of reservoirs, as well as facilitating revitalizing mature water foods and, more importantly, facilitating the design and implementation of an appropriate IOR pilots. The presented reliable method could provide certain significance for the efficient development of offshore oilfields.Cited as: Chang, H., Liu, Y., Lei, Y., Zhang, Q. A comprehensive workflow for real time injection-production optimization based on equilibrium displacement. Advances in Geo-Energy Research, 2020, 4(3): 260-270, doi: 10.46690/ager.2020.03.0

Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)

This work was supported by the National Natural Science Foundation of China (Grant no. 31302221, 31172408 and 31272666) and Jiangsu Province (Grant no. BK20171274 and BK2011418), and partially by the Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland (Grant no. K2016-08). QZ was supported by the “Qinglan” project of Jiangsu province of China.Peer reviewedPublisher PD