Bis[μ-4-amino-3,5-bis­(hydroxy­meth­yl)-1,2,4-triazole]bis{bis­[4-amino-3,5-bis­(hydroxy­meth­yl)-1,2,4-triazole]nickel(II)} tetra­nitrate methanol disolvate

The title complex, [Ni2(C4H8N4O2)6](NO3)4·2CH4O, contains a centrosymmetric binuclear nickel(II) complex bridged by a pair of 4-amino-3,5-bis­(hydroxy­meth­yl)-1,2,4-triazole ligands. The separation between the NiII atoms is 3.962 (1) Å. The Ni atoms are in a slightly distorted octa­hedral coordination. Inter­molecular N—H⋯O, N—H⋯N and O—H⋯O hydrogen bonds connect the ligands, solvent mol­ecules and nitrate ions

(E)-4-Bromo-N′-(2-hydr­oxy-1-naphthyl­methyl­ene)benzohydrazide

The title compound, C18H13BrN2O2, was synthesized by the reaction of 2-hydr­oxy-1-naphthaldehyde with 4-bromo­benzohydrazide. This Schiff base mol­ecule has an E configuration about the C=N bond and is almost planar, the dihedral angle between the mean planes through the substituted benzene ring and the naphthyl system being 6.6 (2)°. There is an intra­molecular O—H⋯N hydrogen bond involving the naphthyl hydr­oxy substituent and the N′ atom of the hydrazide group. In the crystal structure, mol­ecules are linked through inter­molecular N—-H⋯O hydrogen bonds to form chains extending along the b direction

The FEM-Prediction on tensile performance of woven membrane materials under uni and Bi-axial loads

In this study, the mechanical model of the woven PVC-coated membrane materials has been built. By the FEM analysis, it was found out that when tensioned under uni-axial loads, the tensile modulus in the warp and fill direction of woven membrane materials could be predicted nicely, especially after the revision of the properties for the fiber materials. The effect of the tensile moduli of the fiber and the PVC coating materials on the modulus of the woven membrane fabrics has been discussed. It could be consulted that with the proper improvement of the modulus of the fiber materials in the fill direction, the discrepancy between the modulus of woven membrane materials in the warp and fill direction could be reduced to a certain extent. When it comes to the prediction of the modulus of the woven membrane materials under bi-axial loads, large difference could be noticed between the predicted results and the experimental results, especially in warp direction. This was due to the fact that the mechanical analysis model could only show the differences of the geometry configuration between the warp and fill directions. However, the reinforcement of membrane materials in warp direction during weaving and coating processes has been ignored

Adenovirus-mediated delivery of CALR and MAGE-A3 inhibits invasion and angiogenesis of glioblastoma cell line U87

<p>Abstract</p> <p>Background</p> <p>The management of patients with glioblastoma multiforme is difficult. Poor results have led to a search for novel therapeutic approaches. Gene therapy that could be both anti-invasive and antiangiogenic would be ideal. In this study, we constructed the recombinant adenoviral vector Ad-CALR/MAGE-A3 and evaluated its antitumor effects on glioblastoma in vitro and in vivo.</p> <p>Methods</p> <p>In this study, CALR and MAGE-A3 genes were delivered to the glioblastoma cell line U87, using adenovirus (Ad-CALR/MAGE-A3). U87 glioblastoma cells were transfected with Ad-green fluorescent protein to identify the multiplicity of infection. The expressions of CALR and MAGE-A3 were detected by PCR and Western blot. Cell proliferation was measured by MTT assay. Cell apoptosis was assessed by Annexin-V FITC/PI double staining flow cytometry. The invasive potential of U87 cells was determined by Matrigel invasion assay. Tube formation assay was used to detect the effects on angiogenesis of human umbilical vein endothelial cells. Protein expressions of PI3K/AKT, Erk1/2 and MMP-2/-9 in transfected cells were detected by Western blot. In vivo, the effects of Ad-CALR/MAGE-A3 on tumor growth and angiogenesis of U87 glioblastoma xenografts in nude mice were investigated.</p> <p>Results</p> <p>The expressions of CALR and MAGE-A3 in U87 cells resulted in the suppression of cell proliferation and invasion properties, and induced cell apoptosis. The Erk MAPK, PI3K/AKT pathways and expressions of MMP-2/-9 were inhibited in Ad-CALR/MAGE-A3-transfected cells. Outcomes of the tube formation assay confirmed the antiangiogenic effect of CALR. Moreover, in the in vivo model of glioblastoma, intratumoral injection of Ad-CALR/MAGE-A3 suppressed tumor growth and angiogenesis.</p> <p>Conclusion</p> <p>Although Ad-CALR/MAGE-A3 and Ad-CALR demonstrated antiangiogenic effects on U87 cells, the repression of invasion was significant only in Ad-CALR/MAGE-A3-treated cells. To our knowledge, this is the first description of a role for combined CALR and MAGE-A3 in the anti-invasion and antiangiogenesis of U87.</p

Reducing electronic transport dimension to topological hinge states by increasing geometry size of Dirac semimetal Josephson junctions

The notion of topological phases has been extended to higher-order and has been generalized to different dimensions. As a paradigm, Cd3As2 is predicted to be a higher-order topological semimetal, possessing three-dimensional (3D) bulk Dirac fermions, two-dimensional (2D) Fermi arcs, and one-dimensional (1D) hinge states. These topological states have different characteristic length scales in electronic transport, allowing to distinguish their properties when changing sample size. Here, we report an anomalous dimensional reduction of supercurrent transport by increasing the size of Dirac semimetal Cd3As2-based Josephson junctions. An evolution of the supercurrent quantum interferences from a standard Fraunhofer pattern to a superconducting quantum interference device (SQUID)-like one is observed when the junction channel length is increased. The SQUID-like interference pattern indicates the supercurrent flowing through the 1D hinges. The identification of 1D hinge states should be valuable for deeper understanding the higher-order topological phase in a 3D Dirac semimetal