Simple and efficient methods for isolation and activity measurement of the recombinant hirudin variant 3 from Bacillus subtilis

A simple purification approach of the recombinant hirudin variant 3 from the Bacillus subtilis was established, by which the hirudin could be purified to the purity of 95% through one-step chromatography with the total recovery rate of 83.9%. A modified Markwardt thrombin titration method for measuring hirudin activity was also set up. Briefly, a series of concentrations of thrombin was prepared and titrated to hirudin sample, respectively and the anti-thrombin activity-range of hirudin was narrowed down by several thrombin solutions at high or low concentration and the optimum group of thrombin concentrations was determined for titration of the hirudin sample. In this modified method, the hirudin activity was determined more accurately, concisely and promptly than the classic Markwardt method.Key words: Hirudin, thrombin titration method, chromatography, purification

Interaction of a novel red-region fluorescent probe, Nile Blue, with DNA and its application to nucleic acids assay

A novel fluorimetric method was developed for the rapid determination of DNA and RNA based on their quenching effect on the cationic red-region fluorescent dye Nile Blue (NB) In the investigation of the interaction of NE with DNA by steady-state polarization measurements, thermal denaturing study, determination of absorption and fluorescence characteristics, salt effect study and electrophoresis experiments, the results supported the suggestion that NE served as an intercalator to the stack base pairs of nucleic acids. Further evidence showed that the quenching could be ascribed to the static quenching mode. A binding constant of about 10(6) M-1 and a binding site size of about three base pairs were obtained by spectral methods. Under optimum conditions, the calibration curves for the determination of calf thymus DNA (CT DNA) and yeast RNA were linear over the ranges 3.0 ng mL(-1)-2.0 mu g mL(-1) and 27 ng mL(-1)-10 mu g mL(-1), respectively, The detection limits were 3.0 ng mL(-1) for CT DNA and 27 ng mL(-1) for RNA. The relative standard deviation (n = 6) was within 2.1% in the middle of the linear range. Interferences from some interesting co-existing substances in the determination of DNA were also examined

Centralizer's applications to the (b, c)-inverses in rings

[EN] We give several conditions in order that the absorption law for one sided (b,c)-inverses in rings holds. Also, by using centralizers, we obtain the absorption law for the (b,c)-inverse and the reverse order law of the (b,c)-inverse in rings. As applications, we obtain the related results for the inverse along an element, Moore-Penrose inverse, Drazin inverse, group inverse and core inverse.This research is supported by the National Natural Science Foundation of China (no. 11771076 and no. 11871301). The first author is grateful to China Scholarship Council for giving him a scholarship for his further study in Universitat Politecnica de Valencia, Spain.Xu, S.; Chen, J.; Benítez López, J.; Wang, D. (2019). Centralizer's applications to the (b, c)-inverses in rings. Revista de la Real Academia de Ciencias Exactas, Físicas y Naturales. 113(3):1739-1746. https://doi.org/10.1007/s13398-018-0574-0S173917461133Baksalary, O.M., Trenkler, G.: Core inverse of matrices. Linear Multilinear Algebra 58(6), 681–697 (2010)Benítez, J., Boasso, E.: The inverse along an element in rings with an involution, Banach algebras and $$C^*$$ C ∗ -algebras. Linear Multilinear Algebra 65(2), 284–299 (2017)Benítez, J., Boasso, E., Jin, H.W.: On one-sided $$(B, C)$$ ( B , C ) -inverses of arbitrary matrices. Electron. J. Linear Algebra 32, 391–422 (2017)Boasso, E., Kantún-Montiel, G.: The $$(b, c)$$ ( b , c ) -inverses in rings and in the Banach context. Mediterr. J. Math. 14, 112 (2017)Chen, Q.G., Wang, D.G.: A class of coquasitriangular Hopf group algebras. Comm. Algebra 44(1), 310–335 (2016)Chen, J.L., Ke, Y.Y., Mosić, D.: The reverse order law of the $$(b, c)$$ ( b , c ) -inverse in semigroups. Acta Math. Hung. 151(1), 181–198 (2017)Deng, C.Y.: Reverse order law for the group inverses. J. Math. Anal. Appl. 382(2), 663–671 (2011)Drazin, M.P.: Pseudo-inverses in associative rings and semigroups. Am. Math. Mon. 65, 506–514 (1958)Drazin, M.P.: A class of outer generalized inverses. Linear Algebra Appl. 436, 1909–1923 (2012)Drazin, M.P.: Left and right generalized inverses. Linear Algebra Appl. 510, 64–78 (2016)Jin, H.W., Benítez, J.: The absorption laws for the generalized inverses in rings. Electron. J. Linear Algebra 30, 827–842 (2015)Johnson, B.E.: An introduction to the theory of centralizers. Proc. Lond. Math. Soc. 14, 299–320 (1964)Ke, Y.Y., Cvetković-Ilić, D.S., Chen, J.L., Višnjić, J.: New results on $$(b, c)$$ ( b , c ) -inverses. Linear Multilinear Algebra 66(3), 447–458 (2018)Ke Y.Y., Višnjić J., Chen J.L.: One sided $$(b,c)$$ ( b , c ) -inverse in rings (2016). arXiv:1607.06230v1Liu, X.J., Jin, H.W., Cvetković-Ilić, D.S.: The absorption laws for the generalized inverses. Appl. Math. Comput. 219, 2053–2059 (2012)Mary, X.: On generalized inverse and Green’s relations. Linear Algebra Appl. 434, 1836–1844 (2011)Mary, X., Patrício, P.: Generalized inverses modulo $$\cal{H}$$ H in semigroups and rings. Linear Multilinear Algebra 61(8), 1130–1135 (2013)Mosić, D., Cvetković-Ilić, D.S.: Reverse order law for the Moore-Penrose inverse in $$C^*$$ C ∗ -algebras. Electron. J. Linear Algebra 22, 92–111 (2011)Rakić, D.S.: A note on Rao and Mitra’s constrained inverse and Drazin’s $$(b, c)$$ ( b , c ) -inverse. Linear Algebra Appl. 523, 102–108 (2017)Rakić, D.S., Dinčić, N.Č., Djordjević, D.S.: Group, Moore–Penrose, core and dual core inverse in rings with involution. Linear Algebra Appl. 463, 115–133 (2014)Wang, L., Castro-González, N., Chen, J.L.: Characterizations of outer generalized inverses. Can. Math. Bull. 60(4), 861–871 (2017)Wei, Y.M.: A characterization and representation of the generalized inverse $$A^{(2)}_{T, S}$$ A T , S ( 2 ) and its applications. Linear Algebra Appl. 280, 87–96 (1998)Xu, S.Z., Benítez, J.: Existence criteria and expressions of the $$(b, c)$$ ( b , c ) -inverse in rings and its applications. Mediterr. J. Math. 15, 14 (2018)Zhu, H.H., Chen, J.L., Patrício, P.: Further results on the inverse along an element in semigroups and rings. Linear Multilinear Algebra 64(3), 393–403 (2016)Zhu, H.H., Chen, J.L., Patrício, P.: Reverse order law for the inverse along an element. Linear Multilinear Algebra 65, 166–177 (2017)Zhu, H.H., Chen, J.L., Patrício, P., Mary, X.: Centralizer’s applications to the inverse along an element. Appl. Math. Comput. 315, 27–33 (2017)Zhu, H.H., Zhang, X.X., Chen, J.L.: Centralizers and their applications to generalized inverses. Linear Algebra Appl. 458, 291–300 (2014

Wettability of amorphous and nanocrystalline Fe78B13Si9 substrates by molten Sn and Bi

The wettability of amorphous and annealing-induced nanocrystalline Fe78B13Si9 ribbons by molten Sn and Bi at 600 K was measured using an improved sessile drop method. The results demonstrate that the structural relaxation and crystallization in the amorphous substrates do not substantially change the wettability with molten Bi because of their invariable physical interaction, but remarkably deteriorate the wettability and interfacial bonding with molten Sn as a result of changing a chemical interaction to a physical one for the atoms at the interface

Multicolor Combinatorial Probe Coding for Real-Time PCR

The target volume of multiplex real-time PCR assays is limited by the number of fluorescent dyes available and the number of fluorescence acquisition channels present in the PCR instrument. We hereby explored a probe labeling strategy that significantly increased the target volume of real-time PCR detection in one reaction. The labeling paradigm, termed “Multicolor Combinatorial Probe Coding” (MCPC), uses a limited number (n) of differently colored fluorophores in various combinations to label each probe, enabling one of 2n-1 genetic targets to be detected in one reaction. The proof-of-principle of MCPC was validated by identification of one of each possible 15 human papillomavirus types, which is the maximum target number theoretically detectable by MCPC with a 4-color channel instrument, in one reaction. MCPC was then improved from a one-primer-pair setting to a multiple-primer-pair format through Homo-Tag Assisted Non-Dimer (HAND) system to allow multiple primer pairs to be included in one reaction. This improvement was demonstrated via identification of one of the possible 10 foodborne pathogen candidates with 10 pairs of primers included in one reaction, which had limit of detection equivalent to the uniplex PCR. MCPC was further explored in detecting combined genotypes of five β-globin gene mutations where multiple targets were co-amplified. MCPC strategy could expand the scope of real-time PCR assays in applications which are unachievable by current labeling strategy

Enhanced Luminescence of Eu-Doped TiO2Nanodots

Monodisperse and spherical Eu-doped TiO2nanodots were prepared on substrate by phase-separation-induced self-assembly. The average diameters of the nanodots can be 50 and 70 nm by changing the preparation condition. The calcined nanodots consist of an amorphous TiO2matrix with Eu3+ions highly dispersed in it. The Eu-doped TiO2nanodots exhibit intense luminescence due to effective energy transfer from amorphous TiO2matrix to Eu3+ions. The luminescence intensity is about 12.5 times of that of Eu-doped TiO2film and the luminescence lifetime can be as long as 960 μs

Characterization of High-Fat, Diet-Induced, Non-alcoholic Steatohepatitis with Fibrosis in Rats

An ideal animal model is necessary for a clear understanding of the etiology, pathogenesis, and mechanisms of human non-alcoholic steatohepatitis (NASH) and for facilitating the design of effective therapy for this condition. We aimed to establish a rat model of NASH with fibrosis by using a high-fat diet (HFD). Male Sprague–Dawley (SD) rats were fed a HFD consisting of 88 g normal diet, 10 g lard oil, and 2 g cholesterol. Control rats were fed normal diet. Rats were killed at 4, 8, 12, 16, 24, 36, and 48 weeks after HFD exposure. Body weight, liver weight, and epididymal fat weight were measured. Serum levels of fasting glucose, triglyceride, cholesterol, alanine aminotransferase (ALT), free fatty acids (FFA), insulin, and tumor necrosis factor-alpha (TNF-α) were determined. Hepatic histology was examined by H&E stain. Hepatic fibrosis was assessed by VG stain and immunohistochemical staining for transforming growth factor beta 1 (TGF-β1), and alpha-smooth-muscle actin (α-SMA). The liver weight and liver index increased from week 4, when hepatic steatosis was also observed. By week 8, the body weight and epididymal fat weight started increasing, which was associated with increased serum levels of FFA, cholesterol, and TNF-α, as well as development of simple fatty liver. The serum ALT level increased from week 12. Steatohepatitis occurred from weeks 12 through 48. Apparent hepatic perisinosodial fibrosis did not occur until week 24, and progressed from week 36 to 48 with insulin resistance. Therefore, this novel model may be potentially useful in NASH study

A virtual reality collaborative planning simulator and its method for three machines in a fully mechanized coal mining face

The existing automatic control program and its parameters for three machines in a fully mechanized Coal Mining face are static and simplex and are therefore inadequate for satisfying the complex and dynamic environment of underground coal mines. To overcome this problem, a collaborative mathematical model is established that includes the effects of a dynamic environment. A virtual reality collaborative planning simulator with methods for the three machines is also proposed based on a multi-agent system. According to the dynamic characteristics of the environment, equipment, and technologies, a fully mechanized Unity3D simulator (FMUnitySim) is designed in terms of multiple factors and multiple dimensions. The factors affecting the coordinated operation of the three machines are analyzed and modeled. The communication modes, coordination, and redundant sensing process among multiple agents, which include the shearer agent and the scraper conveyor agent, are also investigated in detail. Using this system, the key parameters of the three machines can be planned and adjusted online to design and distinctly observe the corresponding collaborative simulations of coordinated operation with multiple perspectives and in real time. Tests of different maximum shearer haulage speeds for regular or reverse transporting coal are designed; their key parameters, including the average shearer haulage speed, average follower distance, and average scraper conveyor load, are planned and simulated using FMUnitySim. The optimal parameter combination is obtained by analyzing and comparing the simulation results. The proposed FMUnitySim offers an effective means and theoretical basis for the rapid planning and safe automatic production of a fully mechanized Coal Mining face