A dielectrophoresis-impedance method for protein detection and analysis

Dielectrophoresis (DEP) has increasingly been used for the assessment of the electrical properties of molecular scale objects including proteins, DNA, nanotubes and nanowires. However, whilst techniques have been developed for the electrical characterisation of frequency-dependent DEP response, biomolecular study is usually limited to observation using fluorescent markers, limiting its applicability as a characterisation tool. In this paper we present a label-free, impedance-based method of characterisation applied to the determination of the electrical properties of colloidal protein molecules, specifically Bovine Serum Albumin (BSA). By monitoring the impedance between electrodes as proteins collect, it is shown to be possible to observe multi-dispersion behaviour. A DEP dispersion exhibited at 400 kHz is attributable to the orientational dispersion of the molecule, whilst a second, higher-frequency dispersion is attributed to a Maxwell-Wagner type dispersion; changes in behaviour with medium conductivity suggest that this is strongly influenced by the electrical double layer surrounding the molecule

Teeth and their Substitutes

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A More Robust Ethics: The Weakness and Theistic Dependence of Godless Normative Realism

This dissertation is centered on the metaethics and moral ontology of Erik Wielenberg. The central task of the dissertation is to examine Wielenberg’s metaethical view termed “Godless Normative Realism” and ascertain the ontological implications therein. I contend that Wielenberg’s moral ontology ultimately fails to establish moral realism and aspects of his metaethics are better explained by the existence of God rather than his non-existence. Chapter One presents Erik Wielenberg’s metaethics within the background of the historical progression of moral ontology and contemporary approaches to moral realism. Tracing from ancient foundations of theistic moral realism I show how moral antirealism grew out of the modern period with a contemporary response of non-natural moral realism. It is within this historical context that Wielenberg presents his specific moral ontology which he calls “Godless Normative Realism”. I then provide a few methodological clarifications by which the arguments of chapters 2-4 will develop. Chapter Two addresses Wielenberg’s moral ontology, specifically the existence of the sui generis, metaphysically necessary states of affairs on which moral objectivity rests. Upon critically developing his moral ontology I concentrate on Wielenberg’s view of intrinsic value which gives both foundation and motivation to his ontology. I then show how Wielenberg’s approach utilizes theological principles within his own explanations as well as show how the implications of his approach lend themselves more plausibly to a theistic worldview. I conclude the chapter with two possible theistic approaches that better explain Wielenberg’s moral ontology. Chapter Three addresses the supervenience relationship broadly and the making relationship more specifically in Wielenberg’s approach. I then explain the key problematic issues behind the combination of atheism and supervenience. Again, I show how Wielenberg’s approach utilizes theological principles and incurs implications that lend itself more plausibly to a theistic worldview. I conclude with an argument from moral fine-tuning whereby Wielenberg’s approach is more plausible on a theistic worldview. Chapter Four is concerned with the relationship between moral reasons and moral obligations within Wielenberg’s approach. Two major issues arise within this relationship, that of moral authority and what I term “moral weight”. Additionally, residual implications of Wielenberg’s view of moral obligations are then discussed. Finally, I propose a theistic solution that removes the concerns of moral authority and “moral weight” within the confines of Wielenberg’s greater moral ontology. Chapter Five provides an epilogue which summarizes the entirety of Wielenberg’s moral ontology, its shortcomings, and the advantages of theistic solutions

An algorithm for tracking the position and velocity of multiple neuronal signals using implantable microelectrodes in vivo

Peer reviewedPublisher PD

The expansor secundariorum muscle, with special reference to passerine birds

No Abstract.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50251/1/1050990107_ftp.pd

Functional morphology of the cranio-mandibular complex of the Guira cuckoo (Aves)

The cranio‐mandibular complex is an important structure involved in food capture and processing. Its morphology is related to the nature of the food item. Jaw muscles enable the motion of this complex and their study is essential for functional and evolutionary analysis. The present study compares available behavioral and dietary data obtained from the literature with novel results from functional morphological analyses of the cranio‐mandibular complex of the Guira cuckoo (Guira guira) to understand its relationship with the zoophagous trophic habit of this species. The bite force was estimated based on muscle dissections, measurements of the physiological cross‐sectional area, and biomechanical modeling of the skull. The results were compared with the available functional morphological data for other birds. The standardized bite force of G. guira is higher than predicted for exclusively zoophagous birds, but lower than for granivorous and/or omnivorous birds. Guira guira possesses the generalized jaw muscular system of neognathous birds, but some features can be related to its trophic habit. The external adductor muscles act mainly during food item processing and multiple aspects of this muscle group are interpreted to increase bite force, that is, their high values of muscle mass, their mechanical advantage (MA), and their perpendicular orientation when the beak is closed. The m. depressor mandibulae and the m. pterygoideus dorsalis et ventralis are interpreted to prioritize speed of action (low MA values), being most important during prey capture. The supposed ecological significance of these traits is the potential to widen the range of prey size that can be processed and the possibility of rapidly capturing agile prey through changes in the leverage of the muscles involved in opening and closing of the bill. This contributes to the trophic versatility of the species and its ability to thrive in different habitats, including urban areas.Fil: Pestoni, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Degrange, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Tambussi, Claudia Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Demmel Ferreira, María Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Ciencias de la Tierra. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Investigaciones en Ciencias de la Tierra; ArgentinaFil: Tirao, German Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentin

A rich locality in South Kensington: the fossil hominin collection of the Natural History Museum, London

The primacy of fossils in the Natural History Museum (NHM) goes back to the very origins of the Museum, but the first fossil hominins in the collections were probably the Upper Palaeolithic remains from Bruniquel, which were accessioned in 1864. This founded a collection which has continued to expand into this century. While there have been many compilations and descriptions of the fossil hominin collection at the NHM over its long history, to our knowledge no‐one has prepared a review of the collection itself. The intention of the current paper is to synthesize earlier sources with accounts of new finds, revised chronologies and rediscovered treasures in order to illustrate the breadth and continuing importance of the fossil hominins curated at the NHM. We list and discuss all the hominin material known or thought to pre‐date the Holocene. These form a collection of great importance, both in terms of their research