Granulocyte-activating mediators (GRAM)

In the present study we investigated the capability of human epidermal cells to generate granulocyte-activating mediators (GRAM). It could be shown that human epidermal cells as well as an epidermoid carcinoma cell line (A431) produce an epidermal cell-derived granulocyte-activating mediator (EC-GRAM) which stimulates human granulocytes to release significant levels of toxic oxygen radicals as measured by a lucigenin-dependent chemiluminescence (CL). For further characterization of EC-GRAM the A431 cell line was used. Supernatants of A431 cells usually contained maximal EC-GRAM levels within 24 h of incubation. Factor production was enhanced by bacterial lipopolysaccharide (LPS), but not by silica particles and PHA. Moreover, freeze-thaw lysates of A431 cells and extracts of heat-separated human epidermis contained significant levels of EC-GRAM. Preincubation of granulocytes with EC-GRAM resulted in an enhanced response to subsequent stimulation with the chemotactic peptide f-met-phe. In contrast EC-GRAM did not affect the response to PMA or zymosan particles. However, EC-GRAM treated granulocytes were unresponsive to restimulation with EC-GRAM. Upon high performance liquid chromatography (HPLC) gel filtration EC-GRAM eluted within two major peaks exhibiting a molecular weight of 17 kD and 44 kD. According to its biochemical and biological properties EC-GRAM can be separated from other cytokines such as ETAF/-interleukin 1, interleukin 2, interferons, granulocyte colony-stimulating factor (G-CSF) and tumor necrosis factor (TNF). However, an antibody to human GM-CSF neutralized about 75% of the activity. These results indicate that EC-GRAM activity stimulating the generation of reactive oxygen species by granulocytes is probably due to GM-CSF

Seiberg-Witten map for noncommutative super Yang-Mills theory

In this letter we derive the Seiberg-Witten map for noncommutative super Yang-Mills theory in Wess-Zumino gauge. Following (and using results of) hep-th/0108045 we split the observer Lorentz transformations into a covariant particle Lorentz transformation and a remainder which gives directly the Seiberg-Witten differential equations. These differential equations lead to a theta-expansion of the noncommutative super Yang-Mills action which is invariant under commutative gauge transformations and commutative observer Lorentz transformation, but not invariant under commutative supersymmetry transformations: The theta-expansion of noncommutative supersymmetry leads to a theta-dependent symmetry transformation. For this reason the Seiberg-Witten map of super Yang-Mills theory cannot be expressed in terms of superfields.Comment: 9 page

Permutation actions on Quiver Grassmannians for the equioriented cycle via GKM-theory

In our previous work, we equipped quiver Grassmannians for nilpotent representations of the equioriented cycle with an action of an algebraic torus. We show here that the equivariant cohomology ring is acted upon by a product of symmetric groups and we investigate this permutation action via GKM techniques. In the case of (type A) flag varieties, or Schubert varieties therein, we recover Tymoczko's results on permutation representations

Perturbative Chern-Simons Theory on Noncommutative R^3

A U(N) Chern-Simons theory on noncommutative $\mathbb{R}^{3}$ is constructed as a \q-deformed field theory. The model is characterized by two symmetries: the BRST-symmetry and the topological linear vector supersymmetry. It is shown that the theory is finite and \q_{\m\n}-independent at the one loop level and that the calculations respect the restriction of the topological supersymmetry. Thus the topological \q-deformed Chern-Simons theory is an example of a model which is non-singular in the limit \q \to 0.Comment: 10 pages, 3 figures. Added loop calculation, conclusions unchanged, some references adde