Ovulatory Follicular Dynamics After Estrus Synchronization Using Intravaginal Progesterone Implant in Dairy Cows

The study aimed to follow ovulatory follicular dynamics and plasma progesterone profile after estrus synchronization using progesterone intravaginal implant CIDR and its combination with PGF2a and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were divided into 3 treatment groups. Treatment 1, CIDR implant was inserted intravaginally for 9 days (CIDR); treatment 2, given CIDR and 250 mg GnRH intramuscularly at implant removal (CIDR + GnRH); treatment 3, CIDR and 25 mg PGF2a at day 7 of implant insertion (CIDR + PGF2a). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz transducer was performed everyday for 12 days to follow ovulatory follicular dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data were tested using analysis of variance and correlation analysis. The result indicated that CIDR implant was a potent agent for estrus synchronization with the onset of estrus was 66.18 + 03.42 hours after its removal. The addition of GnRH resulted the highest ovulatory growth rate following the implant removal (1.67 + 0.17a, 1.93 + 0.13b dan 1.53 + 0.20a mm/day, P < 0.05). Insertion of CIDR hampered dominant follicular development and its removal caused rapid follicular growth and followed by ovulation. Its insertion resulted steady increase in plasma progesterone levels and its removal resulted in sudden decrease in the hormonal levels, induced ovulatory follicular development, followed by estrus and ovulation. The CIDR implant also inhibited luteal growth, caused no effect of PGF2a administration on decrease of plasma progesterone levels. Addition of GnRH at the time of CIDR removal enhanced the highest rate of ovulatory follicular growth

PROFIL PROGESTERON PLASMA PADA SAPI PERAE PENDERITA HIPOFUNGSI OVARIA

ABSTRACT: Hipofungsi ovaria merupakan salah satu penyebab kemajiran terbanyak pada sapi perah. Sejumlah 12 ekor sapi perah penderita hipofungsi ovaria diambil sampel darahnya lewat vena coceygea dan dianalisa kadar progesteron plasmanya dengan menggunakan teknik imunoasai enzim. Hasil analisa menunjukkan bahwa kadar progesteron plasma sapi penderita hipofungsi ovaria sangat rendah, kurang dari 1 ng/ml (rerata 0,53 + 0,32 ng/ml, kadar tertinggi, 0,92 ng/ml dan terendah 0,17 ng/ml). Kata kunci: progesteron plasma, sapi pera

OVULATORY FOLLICULAR DEVELOPMENTS AND PLASMA PROGESTERONE PROFILES IN SUPEROVULATED DAIRY COWS

ABSTRACT: A study concerning follicular development and plasma progesterone concentrations had been conducted in 9 heads of superovulated dairy cows. Superovulatory treatments consisted of treatment I with multiple injection of FSH 6 - 6, 5 - 5. 4 - 4 and 3 - 3 AU with solvent of aquabidesttreatment II with single injection of 30 AU FSH with solvent of P VPand treatment II with single injection of 3,000 IU PMSG followed by 5,000 IU HCG at the time of the first insemination. Embryo recovery and evaluation were done at the day 7 or 8 after insemination. Ovulatory follicular development was monitored using transrectal realtime ultrasonography. Plasma progesterone determination was performed using a microtitre plate enzyme immunoassay technique. Ultrasonographic examination and blood sample collection were done every other .day commencing the day of superovulatory treatment until the time of embryo recovery. Results of the present study showed that superovulatory treatments I and II had the same number of ovulatory follicular development (12.5 I 0.5 and 15.5:t 2.5, P> 0.05), while treatment III had fewer follicular development (ll.0:t 1.0. P 0.05), respectivelywhile in treatment III had lower concentration 4.2:t 1.3 ng/ml (P < 0.05). The higher incidence of anovulatory follicle and lower embryo quality in treatment III seemed due to this lower plasma progesterone level. Key words: embryo trsanfer, superovulation, follicular development, ovulatory follicle, plasma progesteron

Improvement of reproductive status of bovine embryo transfer recipients by administration of gonadotrophin releasing hormone or human chorionic gonadotrophin

Forty eight Friesian Holstein heifers were used in the study to improve reproductive status of bovine embryo transfer recipients by administration of gonadotrophin-releasing hormone (GnRH) or human chorionic gonadotrophin ( HCG ). Estrus of the recipient was induced by synchronization treatment using 15 nig luprostiol injected intramuscularly (estrus = Day 0). Thawed-frozen embryo was transferred into the apex of cornual horn of recipient at Day 7 of the cycle. Immediately after transfer sixteen recipient heifers were selected randomly as treated group 1 injected intramuscularly with 250 GnRH (gonadorelin), other sixteen heifers as treated group 2 injected intramuscularly with 1,500 IU HCG, while the rest as control animals, given no treatment. Blood samples were collected from all recipients at Days 0, 7, 21, 42, 63 and 84. Blood plasma was separated immediately and stored at temperature of -20°C for quantitative progesterone determination using enzyme immunoassay (EIA) technique as well as for qualitative analysis. Rectal pregnancy examination of recipient heifers were carried out at Day 63, assisted with ultrasonographic examinations and confirmed further with progesterone determinations. Conception rates of the treated and control animals were 50%, 50% and 31.25%, respectively. Blood plasma progesterone levels of the treated animals were consistently higher than that of the control at Days 21, 42, 63, and 84, were 6.12 ±0.98", 6.07 ± 1.08" and 4.43 ± 0.38\u278.28 ± 115\u27, 8.25 ± 1.32" and 6.62 ± 1.19\u278.62 ± 1.28", 8.42 ± 1.47^ and 6.46 ± 1.62\u278.70 ±1.62", 8.33 ±1,15" and 6.87 ±1.48\u27 ng/ml (P < 0.05), respectively. The qualitative progesterone determinations were matched perfectly with the quantitative ones. It can be concluded that the administration of GnRH or HCG to the recipient heifers increases the blood plasma progesterone profiles and improves the conception rates of embryo transfer, without creating any abnormalities for non-pregnant recipients. Application of progesterone determination to confirm the ovarian status and estrus detection can be utilized for optimizing bovine embryo transfer results. Keywords : HCG - GnRH - progesterone recipient - embryo transfer - conception rate

Ovulatory Follicular Dynamics After Estrus Synchronization Using Prostaglandin F2a in Dairy Cows

The study aimed to follow development of ovulatory follicular dynamics as well as plasma progesterone profile after estrus synchronization using PGF2 and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were used in the present study. Treatment 1, given intramuscular injection of PGF2 25 mg (PGF2), treatment 2 PGF2 25 mg and GnRH 250 g 2 days later (PGF2-GnRH), and treatment 3 with GnRH 250 g (7 days prior to injection of PGF2), PGF2 25 mg and GnRH 250 g (2 days after injection of PGF2) (GnRH-PGF2a-GnRH) (the Ovsynch method). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz tranducer was performed everyday for 12 days to follow ovulatory follicular and luteal dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data of follicular, luteal development and progesterone levels were tested using analysis of variance and correlation analysis. The animals showed estrus within 70.70 + 01.90 hours following PGF2 injection. Prostaglandin F2 induced corpus luteum regression, decreased in progesterone plasma levels, followed by ovulatory follicular development and eventually underwent ovulation. Administration of first GnRH increased corpus luteum size, enhanced its regression and decreased plasma progesterone levels, while the second administration induce better ovulatory follicular development. Rate of the corpus luteum regression, progesterone decrease and ovulatory follicular development following PGF2 injection for respective treatments 1, 2 and 3 were 2.53 + 0.24a, 2.73 + 0.36a and 3.53 + 0.28b mm/day; 1.39 + 0.14a, 1.35 + 0.18a dan 1.57 + 0.12b ng/ml/day; and 1.33 + 0.15a, 1.63 + 0.19b and 1.67 + 0.23b mm/day, respectively (P < 0.05). It can be concluded that PGF2 induced corpus luteum regression, decreased in progesterone plasma levels and ovulatory follicular development. Addition of GnRH increased corpus luteum size and plasma progesterone levels, after PGF2 injection corpus luteum regression and progesterone decrease became more prominent, while ovulatory folliculkar development occurred much better.

PENGGUNAAN PMSG UNTUK MEMACU FUNGSI OVARIUM SAPI PERAH PASCA BERANAK

ABSTRAK Sejumlah 16 ekor sapi perah yang telah beranak di Kecarnatan Camping dan Turi, Sleman, Yogyakarta telah dipakai sebagai penelitian pendahuluan (preliminary study) untuk mengetahui pengaruh PMSG terhadap aktivitas ovarium. Sapi tersebut telah diamati lebih dari 4 bulan pasca beranak tidak menunjukkan gejala birahi. Penelitian ini mempunyth 2 kelompok perlakuan dan masing-masing perlakuan terdiri dari 8 ekor sapi perah. Kelompok pertama mewakili sapi perah penderita hipofungsi ova rium dan kelompok kedua mewakili sapi perah birahi tenang. Hasil penelitian menunjukkan bahwa suntikan PMSG dengan dosis 1000 IU dapat menyebabkan 100% sapi perah birahi. Sapi perah kelompok penderita birahi tenang dapat diinduksi birahi PMSG lebih cepat (P ( 0,05) jika dibandingkan dengan kelompok sapi perah penderita hipofungsi ovarium (2,3 kontra 5,1 hari). Sedangkan lama birahi dari kedua kelompok hewan percobaan tidak dipengaruhi oleh pemberian PMSG. Keywords: PMSG, Ovarium sapi pera

Characteristics of Frozen-thawed Semen on Simmental and Limousin Bulls in Ungaran, Indonesia

The present research aimed to study the characteristics of frozen-thawed semen in beef bulls ex-import in Ungaran, Indonesia. 5 heads Simmental and 5 heads Limousin of 5.5 years old were used in this research. The research was done in Ungaran Artificial Insemination Center, Central Java, Indonesia and Laboratory of Reproduction and Obstetry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia. Frozen semen was obtained from this AI center. Results of the research indicated that the average individual motility of Simmental and Limousin is 39.00±5.48% and 36.00±2.24%. The average percentage of live spermatozoa is 54.00±10.75% for Simmental and 48.20±9.78% for Limousin while the average proportion of abnormal spermatozoa is 9.60±3.36% and 6.80±4.15% for both Simmental and Limousin, respectively. On average, Simmental frozen-thawed semen have the higher mean proportion of sperm motility, live sperm, and abnormal sperm than that of Limousin frozen-thawed seme

The Traits of Fresh and Frozen Semen on Brahman Bulls

The present study aimed to determine quality and parameters traits of both fresh and frozen semen in 4-year old Brahman bulls. Semen was collected from 5 bulls twice a week using artificial vagina in Ungaran ArtificialInsemination Center, Central Java, Indonesia. Immediately after collection, samples were evaluated for semen volume, color, odor, viscosity, mass movement, individual movement, spermatozoa concentration and livespermatozoa percentage. Sperm concentration was measured using a spectrophotometer. Good quality fresh semen was processed to be frozen semen. Parameters evaluated for frozen semen include spermatozoa individualmovement, live spermatozoa, and abnormal spermatozoa percentage. The results showed that the semen volume is 6.90 ± 0.54 whereas the average of sperm concentration, individual motility and live sperm are 1754.54 ± 212.67;65.50 ± 3.74% and 76.30 ± 2.64%, respectively. Meanwhile, for frozen semen, the average percentage of motility is 38.00 ± 2.74% while the average proportions of live sperm and abnormal sperm are respectively 45.20 ± 9.86% and 6.00 ± 2.83%. In conclusion, in average, all parameters of fresh and frozen semen of Brahman bulls are in normal range. Based on the Indonesian National Standard, frozen semen produced could be used for inseminatio