Water status and yield response to deficit irrigation and fertilization of three olive oil cultivars under the semi-arid conditions of Tunisia

Sustainability of olive production is possible by adopting the modern techniques of irrigation and fertilization. In Tunisia, olive trees are usually cultivated in poor soils, under semi-arid conditions characterized by water scarcity. This study investigated the effects of different water supply and fertilization on leaf water status and crop yield of three different olive oil varieties cultivated in central Tunisia, during four experimental seasons (2014-2017). Three treatments were examined: trees conducted under rainfed conditions (TRF), which represented the control treatment, trees irrigated with 50% ETc (T50) and, finally, trees irrigated with 50% ETc and with additional fertilization (T50F). Leaf water content and potential, yield and water use efficiency have been monitored on three different varieties, Chetoui, Chemlali, and Koroneiki, which are quite typical in the considered region. For all the growing seasons, midday leaf water potentials were measured from April to September. Midday leaf water potentials (MLWP) were generally higher for the two irrigated treatments (T50 and T50F) than for non-irrigated trees (TRF). As the season proceeded, MLWPs tended to decrease during summer for all the treatments and varieties. The lowest values were observed for the non-irrigated trees, varying between -3.25 MPa to -4.75 MPa. Relative leaf water content followed the same trends of midday leaf water potentials. Chetoui showed the lowest yield, which did not exceed 1530 Kg/(ha year), even for irrigated and fertilized trees. On the other hand, the yields of Chemlali and Koroneiki, cumulated in the four years, reached the maximum value of about 20 tons/ha. For these two varieties, the cumulated yield obtained in the control treatment (TRF) resulted significantly lower than the corresponding of the other two treatments (T50 and T50F). The highest irrigation water use efficiency (WUE) was estimated for Chemlali (T50) and (TRF). WUE was equal to 1.22 Kg/m3 for Koroneiki under fertigated treatment (T50F). Application of the only water supply (50% ETc) or associated with fertilizer improved the tree water status and increased the productivity of Chemlali and Koroneiki varieties

Ion-exchanged waveguide add/drop filter

An add/drop filter is fabricated using ion-exchanged waveguides and photowritten Bragg gratings. The device exhibits 20 dB extinction ratios and 3 dB bandwidths of 0.4 nm (100 GHz)

Grating formation in BGG31 glass by UV exposure

A three-dimensional index variation grating in bulk BGG31 glass written using neither hydrogen loading nor germanium doping is demonstrated. This material is useful for fabricating ion-exchanged waveguides, and its photosensitivity to ultraviolet (UV) radiation at 248nm has not been previously explored. Intensity measurements of the Bragg diffracted spots indicated a maximum index variation (Delta n) of similar to 4 x 10(-5)

Gratings photowritten in ion-exchanged glass channel waveguides

Gratings are photowritten in ion-exchanged glass channel waveguides. The transmission of these waveguides shows a rejection dip of almost 20dB. The polarisation dependence of these waveguide gratings is measured and discussed

Analysis of memory T lymphocyte activity following stimulation with overlapping HLA-A*2402, A*0101 and Cw*0402 restricted CMV pp65 peptides

The continuous efforts aimed at the identification of new immune epitopes across the MHC system has led to the discovery that more than one peptide may be restricted to the same HLA antigen and function as an immune determinant for that association. The aim of this study was to compare the ability of two overlapping peptides, the nonamer (9-mer) cytomegalovirus (CMV) pp65(341–349 )(QYDPVAALF) and the decamer (10-mer) CMV pp65(341–350 )(QYDPVAALFF), and the esadecamer (16-mer) peptide containing both the 9-mer and 10-mer sequences, CMV pp65(340–355 )(RQYDPVAALFFFDIDL), to stimulate and maintain over time a T cell immune reactivation by HLA-A*2402, A*0101, and Cw*0402 cells from CMV-seropositive subjects. The 9-mer, 10-mer, and 16-mer peptides effectively stimulated CTLs from HLA-A*2402, HLA-A*0101, and HLA-Cw*0402 CMV seropositive donors. This data confirms that both the 9-mer and the 10-mer peptides are promiscuous and are not restricted to a single HLA antigen. CMV pp65(341–349 )and CMV pp65(341–350 )have the ability to produce CMV-specific CTLs in subjects with several different HLA types, presenting a practical advantage over other peptides that are restricted only to a single HLA antigen, and thus being optimal for CMV adoptive immune therapy. Moreover, since the 16-mer peptide encompasses both the 9-mer and 10-mer peptides, it may be better than either of these peptides for CMV adoptive immune therapy

MicroRNA-222 regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells

A number of microRNAs have been shown to regulate skeletal muscle development and differentiation. MicroRNA-222 is downregulated during myogenic differentiation and its overexpression leads to alteration of muscle differentiation process and specialized structures. By using RNA-induced silencing complex (RISC) pulldown followed by RNA sequencing, combined with in silico microRNA target prediction, we have identified two new targets of microRNA-222 involved in the regulation of myogenic differentiation, Ahnak and Rbm24. Specifically, the RNA-binding protein Rbm24 is a major regulator of muscle-specific alternative splicing and its downregulation by microRNA-222 results in defective exon inclusion impairing the production of muscle-specific isoforms of Coro6, Fxr1 and NACA transcripts. Reconstitution of normal levels of Rbm24 in cells overexpressing microRNA-222 rescues muscle-specific splicing. In conclusion, we have identified a new function of microRNA-222 leading to alteration of myogenic differentiation at the level of alternative splicing, and we provide evidence that this effect is mediated by Rbm24 protei

Polarisation-independent Bragg gratings in ion-exchanged glass channel waveguides

The polarisation dependence of Bragg gratings photowritten in ion-exchanged glass waveguides is characterised for waveguides with different mask-opening widths and burial depths. It is found that polarisation-independent gratings can be written in waveguides with a wide variation in fabrication parameters

LE REGIONI D'EUROPA TRA IDENTITÀ LOCALI, NUOVE COMUNITÀ E DISPARITÀ TERRITORIALI

L’Europa è oggi attraversata da una forte domanda di autonomia da parte dei territori: da un lato, a tutela dell’identità delle comunità locali e, dall’altro, per la crescente rivendicazione di una più ampia rappresentanza delle cittadinanze. Queste istanze possono, però, mettere a rischio gli obiettivi di equilibrio e di coesione economica, sociale e territoriale, da sempre al centro della riflessione e delle proposte sviluppate dagli studiosi delle scienze regionali. La recente crisi ha acuito l’instabilità e allargato i complessivi divari tra le regioni, anche nella loro dinamica interna di evoluzione. Alla resilienza economica non sempre è corrisposto, nell’attuale transizione verso un nuovo paradigma, il mantenimento di solidi livelli di coesione sociale e di integrazione tra aree e comunità. Il volume raccoglie una selezione di contributi presentati alla XXXIX Conferenza Italiana di Scienze Regionali, svoltasi a Bolzano il 17, 18 e 19 settembre 2018. Nella sua articolazione, esso si sviluppa in tre parti. La prima analizza il tema della domanda di autonomia da parte dei territori all’interno dello sviluppo economico e sociale di regioni e paesi, e dell’attuale dibattito sul federalismo differenziato. Nella seconda le riflessioni si estendono alla crescente disparità tra aree centrali e aree periferiche, tra aree interne e aree esposte, tra Nord e Sud dei Paesi e dell’Europa, mentre nella terza parte, l’analisi guarda ai mutevoli equilibri tra territori e sistemi locali urbani e rurali, tra pianura e montagna e, all’interno delle aree montane, tra i fondivalle maggiormente urbanizzati e i territori di alta quota, zone spesso di confine tra province, regioni e stati. La novità editoriale di quest’anno è rappresentata da uno spazio dedicato a brevi contributi a cura di giovani autori vincitori dei premi AISRe.Europe is today crossed by a strong demand for autonomy from the territories: to protect the identity of local communities and, also analyze the growing demand for a broader representation of citizens. However, these issues can jeopardize the objectives of balance and economic, social and territorial cohesion, which have always been at the center of the reflection and proposals developed by scholars of regional sciences. The recent crisis has worsened instability and widened the overall gaps between the regions, including in their internal dynamics of evolution. Economic resilience is not always matched, in the current transition towards a new paradigm, by maintaining solid levels of social cohesion and integration between areas and communities. The volume collects a selection of contributions presented at the XXXIX Italian Conference of Regional Science, held in Bolzano on 17, 18 and 19 September 2018. The volume is divided into three parts. The first analyzes the issue of the demand for autonomy by the territories within the economic and social development of regions and countries, and the current debate on differentiated federalism. In the second, the reflections extend to the growing disparity between central and peripheral areas, between internal and exposed areas, between North and South of countries and Europe, while in the third part, the analysis looks at the changing balance between territories and systems. urban and rural areas, between plains and mountains and, within mountain areas, between the most urbanized valleys and high altitude territories, areas often bordering provinces, regions and states. This year ia space is dedicated to short contributions by young authors who have won the AISRe Awards

LE REGIONI D’EUROPA TRA IDENTITÀ LOCALI, NUOVE COMUNITÀ E DISPARITÀ TERRITORIAL

Europe today is crossed by a strong demand for autonomy from the territories: on the one hand, to protect the identities of local communities and, on the other, for the growing demand for a wider representation of resident citizens. However, these requests can jeopardize the objectives of balance and integration between parts of the territory and between those who live in them, objectives that have always been at the center of reflection and proposals developed in the context of regional sciences. The recent crisis has heightened instability and widened economic and social gaps not only between regions, but also within the regions themselves. The North-South gaps in Italy have further increased and, in a post-crisis phase, this unstoppable process requires ever greater scientific and policy insights in terms of commitment and intervention capacity. If the economic growth of the past decade had found impetus in expanding the spaces of relationships and exchanges, the responses to the threats brought about by the crisis materialized in the closure of borders and in the defense of local levels of well-being. A particular specification of this theme also concerns the relationships between urban and rural territories and local systems, between plains and mountains as well as mountain areas, . The preface is therefore an acute reflection on issues of urgent relevance for Italy and of great interest for the regional sciences, accepting important scientific and policy challenges