7 research outputs found
Giardia duodenalis in Wildlife: Exploring Genotype Diversity in Italy and across Europe
Fragmented data are so far available on genotype diversity of G. duodenalis in wildlife in different countries in Europe, in particular, in Italy. In the present study, G. duodenalis sequences obtained from different Italian wild animals [12 porcupines (Hystrix cristata), 4 wild boars (Sus scrofa), 1 wolf (Canis lupus italicus), 6 Alpine chamois (Rupicapra rupicapra rupicapra)] were compared with those available from wild host species in Europe to add new data on the geographic distribution of Giardia assemblages/sub-assemblages and their transmission patterns among natural hosts. Thirty-eight sequences were obtained by MLG analysis (SSU-rRNA, bg, gdh, and tpi genes) and subsequently compared by phylogenetic and network analyses with those from wild species monitored in the last decades in Europe. The results revealed the presence of potentially zoonotic (A-AI, A-AII from wild boar; B from porcupine) and host-adapted (D from wolf; E, A-AIII from chamois) assemblages and sub-assemblages and represent the first report for Italian wild boar. The analysis did not find any evidence of spatial or host segregation for specific genetic variants, mostly shared between different hosts from different European countries. However, conflicting evidence was found in genotypic assignment, advocating for data improvement and new genomic approaches
Semi-invariants of symmetric quivers of finite type
Let be a symmetric quiver, where is a finite
quiver without oriented cycles and is a contravariant involution on
. The involution allows us to define a nondegenerate bilinear
form on a representation $V$ of $Q$. We shall call the representation
orthogonal if is symmetric and symplectic if is skew-symmetric.
Moreover we can define an action of products of classical groups on the space
of orthogonal representations and on the space of symplectic representations.
For symmetric quivers of finite type, we prove that the rings of
semi-invariants for this action are spanned by the semi-invariants of
determinantal type and, in the case when matrix defining is
skew-symmetric, by the Pfaffians
Semi-invariants of symmetric quivers of tame type
A symmetric quiver is a finite quiver without oriented cycles
equipped with a contravariant involution on . The involution allows us to define a nondegenerate bilinear form on
a representation $V$ of $Q$. We shall say that $V$ is orthogonal if is
symmetric and symplectic if is skew-symmetric. Moreover, we define an
action of products of classical groups on the space of orthogonal
representations and on the space of symplectic representations. So we prove
that if is a symmetric quiver of tame type then the rings of
semi-invariants for this action are spanned by the semi-invariants of
determinantal type and, when matrix defining is skew-symmetric, by
the Pfaffians . To prove it, moreover, we describe the symplectic and
orthogonal generic decomposition of a symmetric dimension vector
First report of Giardia duodenalis infection in the crested porcupine (Hystrix cristata L., 1758)
Italy is the only European country where the crested porcupine (Hystrix cristata) lives. A parasitological investigation was performed on faecal samples, aimed to evaluate Giardia and other parasites in a free-ranging crested porcupine population in Central Italy. Samples were collected from captured and road-killed individuals as well as from feeding areas and pathways. Collected faecal samples were examined by the Mini-FLOTAC technique and a rapid immunoassay for the search of Giardia and Cryptosporidium spp. faecal antigens. For the identification of Giardia species and genotypes, molecular analysis was performed on Giardia-positive samples, by using PCR protocols able to amplify glutamate dehydrogenase, triosephosphate isomerase and a fragment of the small subunit ribosomal RNA genes. A total of 52 crested porcupine faecal samples were collected and analysed. At microscopical examination, 39 out of 52 samples were found positive for at least a single parasite species and six different parasite taxa were identified. Forty-eight percent (25/52) of faecal samples were positive for Giardia spp. and 1.9% (1/52) for Cryptosporidium spp. at the immunoassay. Among 12 faecal samples belonging to different individuals, 33.3% (4/12) were positive for Giardia spp. By using the Mini-FLOTAC technique, positivity for Trichuris spp. (32.7%, 17/52), gastrointestinal strongyles (32.7%, 17/52), capillariid eggs (3.8%, 2/52) and coccidian oocysts (1.9%; 1/52) was also evidenced. Molecular analysis was performed on 17 out of 25 Giardia-positive isolates. At the SSU rDNA locus, expected bands were achieved for 12 out of 17 isolates and all samples were assigned to Giardia duodenalis assemblage B. Sequencing at tpi locus revealed potentially zoonotic G. duodenalis assemblage AII (two isolates) and assemblage BIV (one isolate). The present study provides the first report of G. duodenalis infection in H. cristata. More in depth studies are needed on the impact and epidemiology of G. duodenalis and other identified parasites in crested porcupines