Micropropagation of Azadirachta indica and assessment its fungicidal action

An effective protocol has been developed for micropropagation of Azadirachta indica using Murashige and Skoog ( MS) medium supplemented with different hormones like    1-Naphthyl acetic acid(NAA),3-Indole acetic acid(IAA),3-Indole butyric acid(IBA),6 Benzyl aminopurine (BAP),2,4- dichlorophenoxyacetic acid(2,4-D)and Kinetin.  Extracts obtained were analyzed using HPTLC. Different sample preparations were compared for action against fungi like Aspergillus niger, Penicillium sp, Aspergillus versicolor, Drechslera sp with sample from the original plant. The present in vitro procedure is more effective than the conventional techniques used in conservation and mass propagation of this medicinal plant