N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

Green tea (Camellia sinensis) is one of tradisional plants that have potential as anticancer. Thesample used in this research was commercial green tea extract. The purpose of this study was to test theantiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract wasfractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and water fraction of greentea have Lethality Concentration 50 (LC50) value of more than 1000 ppm. Fractions of ethanol 70% and nhexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of phytochemicalscreening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screeningresults of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancercells MCM-B2 and normal cells Vero by tripan blue staining method. The highest MCM-B2 cell inhibitoryactivity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction,59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Verocells characterized by not inhibiting normal cell proliferation

PF-35 Spider Silk (Nephilia sp.) as Suture Material on Blood Vessel Surgery

Surgical suture is a medical device used to hold body tissues together after an injury or surgery. Application generally involves using a needle with an attached length of thread. Surgical sutures are normally classified into two types, absorbable and non-absorbable. They can also be classified based on their construction, either mono-filament or multi-filament and also whether they are made from natural or synthetic materials. Sutures can also be classified according to their usage e.g. cardiovascular sutures, ophthalmic sutures, general sutures, orthopaedic sutures etc. Common problems associated with the choice of suture material include increased risk of infection, foreign body reactions, and inappropriate mechanical responses, particularly decreases in mechanical properties over time. Improved suture materials are therefore needed. As a high- performance material with excellent tensile strength, spider silk fibres are an extremely promising candidate for use in surgical sutures. However, the biochemical behaviour of individual silk fibres braided together has not been thoroughly investigated. In the present study, we characterise the inflammatory response produced from silk sutures and absorbance time

The effect of excretory/secretory product released by L3 of A. galli on villous compact in intestine of laying hens

The nematode, Ascaridia galli, caused pathological changes in the gut, while the larval development takes place in the intestine epithelium. The aim of this research was to examine the effect of excretory/secretory product released by L3 of A. galli to prevent intestine destructions based on villous compact in duodenum, jejunum, and ileum of laying hens. Excretory/secretory was prepared from metabolic of L3 released in culture médium in flasks containingrosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Excretory/secretory product was concentrated with vivaspin 30.000 MWCO. The chickens vaccinated with 80 g protein excretory/secretory in crudemixed with Fruend Adjuvant Complete and repeated three times with dose of each 60 g mixed with Freund Adjuvant Incomplete with an interval of one week intra muscularly. The chickens were challenged with 1000 infective larvae A. galli one week later. After two weeks the chickens were operated. Villous compact in duodenum, jejunum, and ileum of laying hens were analyzed. The result showed that immunization was able to sustained significantly intestinevillus compact, particularly in duodenum. This research concluded that the excretory/secretory product could protect villus in duodenum against parasitic diseases caused by A. galli

Message from Orginizing Committee

Dear Colleagues,     It gives us immense pleasure to inform you that Indonesian Veterinary Medical Association (IVMA) is organizing the 20th Federation of Asian Veterinary Associations Congress (FAVA Congress) & the 18th Indonesian Veterinary Medical Association Congress (IVMA Congress) an International Congress, Seminar and Workshop. The event will be held in the Nusa Dua, Bali, the very famous paradise island and international tourist destination from October 30th to November 3rd, 2018.     The current congress will be enriched and compose of several satellite meeting of international and national veterinary organizations such as The 40th FAVA Council Meeting, 15th IVMA International Veterinary Scientific Conference and Animal Health Expo, The 2nd JSPS Core-to-Core Program Tripartite Meeting, The 1st FAVA-AVA Joint Meeting, ASEAN Veterinary Statutory Body Network (AVSBN) Meeting, Meeting of IVMA's Organizations of Veterinary Specialty and Scientific Interest Groups, Indonesian Animal Hospital Association (ARSHI) Meeting and the 16th PIDHI Meeting. It is expected to be a big event in veterinary field in 2018 with expected more than 1,000 participants coming around the country as well as foreign participants from Asian countries and other continents.     In this opportunity, a very basic Veterinary Role as an important profession was chosen as the theme of the present event “To Serve Mankind through Animal Kingdom”. The Organizing Committee cordially invites all the Veterinarian, Veterinary and Biomedical Scientists, Animal Husbandry practitioners, Human Medical, Academicians, Scholars and other related professions in Asia and other continents to take part in this event.     The Congress and Scientific seminar will focus on the improvement of the role of veterinary professions in Asian countries as well as recent developments, trends and technologies available for disease prevention, diagnosis, treatments as well as productivity and quality of animals and animal products. The main objective of this event is to bridging government-academicians–business sector and public participation to achieve the goal for better serve and better quality of life. Thank you.Sincerely, Bambang Pontjo Priosoeryanto, Chairman of the Organizing Committe

Mucosal Mast Cells Response in the Jejunum of Ascaridia galli-Infected Laying Hens

Intestinal defense mechanism against helminthes parasitic nematode to be associated with mucosal mast cells reaction. The aim of this research was to examine the effect of infection by Ascaridia galli parasite to trigger mucosal defense based on mucosal mast cells response in laying hens. Amount of ten head laying hens 12-wk old were divided into two groups containing five chickens of each. The first group, chickens were left as un-infected controls. The second group, chickens were infected orally with 1,000 embryonated eggs of A. galli.  Mucosal mast cell responses were assayed by in situ jejunal mast cell counts in stained serial histological sections with Alcian blue (pH 0.3) and Safranin-O (pH 0.1) of the jejunum. Mucosal mast cells response were observed and counted on days 14 post infection. The result showed that A. galli infection was able to increase significantly (P<0.05) mast cells response. This research concluded that the A. galli infection can trigger the involment of mucosal mast cells response in jejunal defense of laying hens against parasitic diseases caused by A. galli

Potensi Tanaman Alpukat (Persea americana Mill) dalam Bidang Dermatologi

Penggunaan obat tradisional yang berasal dari tanaman dapat menjadi alternatif untuk mengatasi masalah kulit. Tanaman alpukat (Persea americana Mill.) diketahui memiliki banyak khasiat karena mengandung flavonoid, saponin, tannin, alkaloid, steroid, dan fenol yang merupakan antioksidan yang berperan dalam menangkal radikal bebas, sehingga dapat digunakan sebagai alternatif terapi pada beberapa kelainan kulit, serta menjaga kualitas dan penampilan kulit. Metode yang digunakan oleh penulis adalah kajian literatur dari jurnal nasional maupun internasional. Artikel dan jurnal yang didapat dibaca, ditelaah dan dianalisis untuk memberikan informasi baru yang relevan. Hasil penelusuran didapatkan bahwa tanaman alpukat, baik dari daging buah, daun, biji dan kulit memiliki khasiat dalam bidang dermatologi, diantaranya yaitu sebagai antibakteri pada antijerawat, antijamur, tabir surya, agen pemutih, pelembab kulit, dan antiaging. Dapat disimpulkan bahwa tanaman alpukat dapat digunakan sebagai alternatif untuk mengatasi berbagai masalah kulit, walaupun masih harus dilakukan penelitian lebih lanjut untuk menggali lebih banyak manfaat lain dari tanaman alpukat dalam bidang dermatologi. Kata kunci: Alpukat, Persea americana Mill, dermatologi

Profile of blood glucose and ultrastucture of beta cells pancreatic islet in alloxan compound induced rats

Diabetes is marked by elevated levels of blood glucose, and progressive changes of the structure of pancreatic islet histopathology. The objective of this research was to analyse the glucose level and histophatological feature in pancreatic islet in alloxan compound induced rats. A total of ten male Spraque Dawley rats of 2 months old were used in this study. The rats were divided into two groups: (1) negative control group (K-), and (2) positif induced alloxan group (diabetic group =DM). The rats were induced by a single dose intraperitonial injection of alloxan compound 120 mg/kg of body weight. The treatment was conducted for 28 days. Blood glucose levels of rats were analysed at 0, 4, 7, 14, 21, and 28 days following treatment. At the end of the experiment, rats were sacrificed by cervical dislocation. Pancreas was collected for analysis of histopathological study by Immunohistochemical technique, and ultrastructural study using transmission electron microscope (TEM). The result showed that Langerhans islet of diabetic rat (rat of DM group) showed a marked reduction of size, number of Langerhans islet of diabetic rat decrease, and characterized by hyperglycemic condition. By using TEM, beta cells of DM group showed the rupture of mitochondrial membrane, the lost of cisternal structure of inner membrane of mitocondria, reduction of insulin secretory granules, linkage between cells acinar with free Langerhans islet, and the caryopicnotic of nucleus. Key words: Alloxan, Beta Cells, Rat, Blood Glucose, Immunohistochemistr

Antiproliferation activity of water and ethyl acetate red yeast rice fraction against MCM-B2 tumor cells

Red yeast rice (RYR), commonly known as angkak, is a functional food fermented by rice using Monascus mushrooms. It has a pigment and flavonoid content that is useful as an antioxidant and plays a role in preventing cancer or tumors. This analysis aims to test the antiproliferation activity of commercial RYR fractions of water and ethyl acetate against Miyazaki Canine Mammary Gland Tumor-Bambang 2 (MCM-B2) breast tumor cells. Research on RYR antiproliferation of cancer-sustaining MCM-B2 cells has not been previously released. In the preliminary study, the antiproliferation bioactivity was measured using the brine shrimp lethality test. The outcome of the brine shrimp lethality test showed that ethyl acetate and water fraction cytotoxicity were more than 1000 ppm and 337.07 ppm, respectively, at a lethal concentration of 50. Antiproliferative activity analyses were performed using direct hemocytometer counting. The antiproliferation activity data collected were analyzed using one-way ANOVA and Duncan continuous testing. The outcome showed that the water and ethyl acetate RYR antiproliferation activity against MCM B2 cancer cells correlated positively with the increasing concentration of each fraction. Ethyl acetate and water fractions at concentrations of 350 ppm may inhibit the growth of MCM-B2 cancer cells in vitro, reaching 42.63 percent and 39.84 percent, respectively, not significantly different (P < 0.05) with a positive doxorubicin control of 41.24 percent. In conclusion, the ethyl acetate and water fraction of RYR have potent antiproliferation activity against MCM-B2 breast tumor cells