An evaluation of the bacterial diversity at Tshipise, Mphephu and Sagole hot water springs, Limpopo Province, South Africa

Tshipise, Mphephu and Sagole are thermal hot water springs in the Limpopo Province of South Africa with temperatures of 58, 43 and 45°C; and pH of 8.85, 8.08 and 9.70, respectively. The bacterial diversity of the hot water springs was determined by pyrosequencing of the two 16S rRNA hypervariable regions V1-3 and V4-7. Analyses of the community DNA revealed that bacterial populations as detectable by the V1-3 or V4-7 region, respectively were dominated by the Bacteriodetes and Proteobacteria for Mphephu, and Proteobacteria and Cyanobacteria for both Tshipise and Sagole. The major differences in the bacterial diversity between the springs was that no Cyanobacteria were detected for Mphephu and the level of Bacteriodetes detected for both Tshipise and Sagole was much lower compared to the levels detected at Mphephu. The Firmicutes were detected at all the springs but at a much lower abundance compared to the other main phyla detected. Various other phyla were detected at the hot springs at levels below 0.20% of the total sequences obtained. It is interesting that very diverse bacterial genera exist in the three hot water springs studied.This research was supported through a grant from Water Research Commission (WRC, SA, Project K5/1959/1).http://www.academicjournals.org/AJM

Beoordelingskriteria vir effektiewe diadiese loopbaanvoorligting

M.A. (Psychology)The thesis was introduced by a description of the historical development of career counselling in South Africa, which indicated that the first traces of career counselling services were present at early times. The description also stressed the importance of career counselling especially during times of work shortage. Present day living costs and shortage of work emphasize the need for effective career counselling to ensure suitable career choices. Currently students are trained In career counselling and evaluated by supervisors without the existence of recognised and tested criteria. To ensure a higher standard of training and practice the implementation of such criteria is of utmost importance in the current developmental phase of career counselling in South Africa. The research aim of this study is the development and evaluation of criteria for effective diadic career counselling. The criteria were specifically designed for students in training and they should form part of the career counselling curriculum. In order to achieve the aim of the study, an integrated theoretical developmental research model was used. The model mainly consists of Thomas's (1984) four-phase model which features analysis, design, development and evaluation phases. Each of the phases contains a number of material conditions as well as methodologies through which these conditions can be met. In the first phase an analysis of the problem was undertaken, the status of existing criteria was investigated and a feasibility study was undertaken. It was then decided to proceed with the design phase. In this phase the following material conditions were met aims and objectives were formulated for the criteria, a practice model was drawn up, a tentative presentation was made of the innovation suggestions, and the innovation procedures were determined