Application of gnawing sticks in rabbit housing

[EN] Four experiments are described relating to gnawing sticks application in rabbit housing. In experiment 1 and 2, Pannon White rabbits, weaned at the age of five weeks, were placed into pens with wire net floor. In experiment 1, every pen (180 animals in 12 pens, surface per head: 571 cm2) was provided with three gnawing sticks randomly chosen from White locust, Black elder, White willow, Little-leaf linden, European larch, Black poplar, European white birch, White buckeye and White mulberry species. In experiment 2 (150 animals in 10 pens, surface per head: 571 cm2), only those tree species ingested by rabbits in the first experiment were used (White locust, White willow, Little-leaf linden, Black poplar and White buckeye). In the second experiment, rabbits showed the highest preference towards gnawing sticks of Little-leaf linden, while similar White willow and White buckeye consumption was observed. In the experiment 3, rabbits¿ preference to different types of wood and the influence on rabbits¿ behaviour of added wooden sticks as environmental enrichment was studied. At the age of 38 days, 48 male SIKA rabbits were housed individually in wire cages (surface per head: 1500 cm2). According to the type of wood placed in the cage, rabbits were equally allotted to four groups: control, Common oak, Little-leaf linden and Norway spruce. Rabbits¿ preference to type of wood was studied on all the animals with wooden stick, while rabbits¿ behaviour was studied on 16 focus animals at the age of 5 and 13 weeks using continuous 24 hours video recordings. Rabbits preferred gnawing Little-leaf linden and Norway spruce compared to Common oak. Addition of gnawing sticks had no significant influence on duration of rabbits¿ behaviour, except for eating feed and gnawing wooden stick time. In experiment 4, the effect of housing and environmental enrichment on the performance and behaviour of growing rabbits was tested. 72 hybrid rabbits were housed after the weaning period in standard fattening cages at 2, 3, 4 animals per cage (surface per head: 1045 cm2, 697 cm2, 522 cm2, respectively). Half cages were enriched using a wood stick (Robinia pseudoacacia) dangling from the ceiling of the cage. The environmental enrichment decreased the stereotypies (gnawing the bars of the cage). A tendency to lower aggressive behaviours in enriched caged rabbits was also found compared to the conventional caged rabbits. This might indicate a better satisfaction of the behavioural needs for the enriched caged rabbits.Princz, Z.; Orova, Z.; Nagy, I.; Jordan, D.; Stuhec, I.; Luzi, F.; Verga, M.... (2007). Application of gnawing sticks in rabbit housing. World Rabbit Science. 15(1):29-36. doi:10.4995/wrs.2007.607293615

A 500-year tale of co-evolution, adaptation, and virulence: Helicobacter pylori in the Americas

Helicobacter pylori is a common component of the human stomach microbiota, possibly dating back to the speciation of Homo sapiens. A history of pathogen evolution in allopatry has led to the development of genetically distinct H. pylori subpopulations, associated with different human populations, and more recent admixture among H. pylori subpopulations can provide information about human migrations. However, little is known about the degree to which some H. pylori genes are conserved in the face of admixture, potentially indicating host adaptation, or how virulence genes spread among different populations. We analyzed H. pylori genomes from 14 countries in the Americas, strains from the Iberian Peninsula, and public genomes from Europe, Africa, and Asia, to investigate how admixture varies across different regions and gene families. Whole-genome analyses of 723 H. pylori strains from around the world showed evidence of frequent admixture in the American strains with a complex mosaic of contributions from H. pylori populations originating in the Americas as well as other continents. Despite the complex admixture, distinctive genomic fingerprints were identified for each region, revealing novel American H. pylori subpopulations. A pan-genome Fst analysis showed that variation in virulence genes had the strongest fixation in America, compared with non-American populations, and that much of the variation constituted non-synonymous substitutions in functional domains. Network analyses suggest that these virulence genes have followed unique evolutionary paths in the American populations, spreading into different genetic backgrounds, potentially contributing to the high risk of gastric cancer in the region.Fil: Muñoz Ramirez, Zilia Y.. INSTITUTO POLITÉCNICO NACIONAL (IPN);Fil: Pascoe, Ben. University of Bath; Reino UnidoFil: Mendez Tenorio, Alfonso. INSTITUTO POLITÉCNICO NACIONAL (IPN);Fil: Mourkas, Evangelos. University of Bath; Reino UnidoFil: Sandoval Motta, Santiago. Consejo Nacional de Ciencia y Tecnología; MéxicoFil: Perez Perez, Guillermo. New York University Langone Medical Center; Estados UnidosFil: Morgan, Douglas R.. University of Alabama at Birmingahm; Estados UnidosFil: Dominguez, Ricardo Leonel. Western Honduras Gastric Cancer Prevention Initiative Hospital de Occidente Santa Rosa de Copan; HondurasFil: Ortiz Princz, Diana. No especifíca;Fil: Cavazza, Maria Eugenia. No especifíca;Fil: Rocha, Gifone. Universidade Federal de Minas Gerais; BrasilFil: Queiroz, Dulcienne. Universidade Federal de Minas Gerais; BrasilFil: Catalano, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Zerbetto de Palma, Gerardo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Goldman, Cinthia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Venegas, Alejandro. Universidad Diego Portales; ChileFil: Alarcon, Teresa. Universidad Autónoma de Madrid; EspañaFil: Oleastro, Monica. Universidade Nova de Lisboa; PortugalFil: Vale, Filipa F.. Universidade Nova de Lisboa; PortugalFil: Goodman, Karen J.. University of Alberta; CanadáFil: Torres, Roberto C.. Instituto Mexicano del Seguro Social; MéxicoFil: Berthenet, Elvire. Swansea University Medical School; Reino UnidoFil: Hitchings, Matthew D.. Swansea University Medical School; Reino UnidoFil: Blaser, Martin J.. Rutgers University; Estados UnidosFil: Sheppard, Samuel K.. University of Bath; Reino UnidoFil: Thorell, Kaisa. University of Gothenburg; SueciaFil: Torres, Javier. Instituto Mexicano del Seguro Social; Méxic

NOTES ON THE STATUS AND DISTRIBUTION OF LITTORINA-FLAVA KING AND BRODERIP, 1832

Volume: 23Start Page: 373End Page: 37

Temporal evolution over ten years in the macrobenthos of muddy sands in the Bay of Concarneau (France)

The temporal evolution of the Amphiura filiformis community of two stations in the Bay of Concarneau was followed during a period of ten years (1970-1979). A concomitance has been found between the demographic fluctuations of the community and the effects of climatic and hydrodynamic factors occurring in the bay throughout the observation period. At the end of the decade a decline of the Amphiura filiformis population and some qualitative changes in the community are observed

The Slx4-Dpb11 scaffold complex: coordinating the response to replication fork stalling in S-phase and the subsequent mitosis.

Replication fork stalling at DNA lesions is a common problem during the process of DNA replication. One way to allow the bypass of these lesions is via specific recombination-based mechanisms that involve switching of the replication template to the sister chromatid. Inherent to these mechanisms is the formation of DNA joint molecules (JMs) between sister chromatids. Such JMs need to be disentangled before chromatid separation in mitosis and the activity of JM resolution enzymes, which is under stringent cell cycle control, is therefore up-regulated in mitosis. An additional layer of control is facilitated by scaffold proteins. In budding yeast, specifically during mitosis, Slx4 and Dpb11 form a cell cycle kinase-dependent complex with the Mus81-Mms4 structure-selective endonuclease, which allows efficient JM resolution by Mus81. Furthermore, Slx4 and Dpb11 interact even prior to joining Mus81 and respond to replication fork stalling in S-phase. This S-phase complex is involved in the regulation of the DNA damage checkpoint as well as in early steps of template switch recombination. Similar interactions and regulatory principles are found in human cells suggesting that Slx4 and Dpb11 may have an evolutionary conserved role organizing the cellular response to replication fork stalling

Renewal and Update of MTO IDF Curves: Defining the Uncertainty

Abstract The University of Waterloo has been commissioned by the Design and Contract Standards Office of the Ministry of Transportation of Ontario (MTO) to update the intensity-duration-frequency (IDF) curves that are used to estimate design storms for drainage infrastructure. Environment Canada provides analyzed rainfall data for weather stations in Ontario. Project engineers determine the design storm parameters using a variety of methods. Thus, there is a need for a consistent interpolation procedure that can deal with the sparseness and unevenness of the Ontario station network. This paper describes the procedures that form the basis for the MTO IDF Curve Lookup system. Confidence limits assist in the design process and allow comparison with other results. These were used to assess the spatial bias in the error fields. There appears to be none, but there is room for improvement where there are sharp changes in geography, such as the highlands west of Thunder Bay. Validation curves are presented for several Canadian stations and one American station. A single set of ten regression equations is sufficient for the whole province, eliminating the need for regional IDF curves and simplifying the design process

A cell cycle-regulated Slx4-Dpb11 complex promotes the resolution of DNA repair intermediates linked to stalled replication

A key function of the cellular DNA damage response is to facilitate the bypass of replication fork-stalling DNA lesions. Template switch reactions allow such a bypass and involve the formation of DNA joint molecules (JMs) between sister chromatids. These JMs need to be resolved before cell division; however, the regulation of this process is only poorly understood. Here, we identify a regulatory mechanism in yeast that critically controls JM resolution by the Mus81-Mms4 endonuclease. Central to this regulation is a conserved complex comprising the scaffold proteins Dpb11 and Slx4 that is under stringent control. Cell cycle-dependent phosphorylation of Slx4 by Cdk1 promotes the Dpb11-Slx4 interaction, while in mitosis, phosphorylation of Mms4 by Polo-like kinase Cdc5 promotes the additional association of Mus81-Mms4 with the complex, thereby promoting JM resolution. Finally, the DNA damage checkpoint counteracts Mus81-Mms4 binding to the Dpb11-Slx4 complex. Thus, Dpb11-Slx4 integrates several cellular inputs and participates in the temporal program for activation of the JM-resolving nuclease Mus81

A cell cycle-regulated Slx4-Dpb11 complex promotes the resolution of DNA repair intermediates linked to stalled replication

A key function of the cellular DNA damage response is to facilitate the bypass of replication fork-stalling DNA lesions. Template switch reactions allow such a bypass and involve the formation of DNA joint molecules (JMs) between sister chromatids. These JMs need to be resolved before cell division; however, the regulation of this process is only poorly understood. Here, we identify a regulatory mechanism in yeast that critically controls JM resolution by the Mus81–Mms4 endonuclease. Central to this regulation is a conserved complex comprising the scaffold proteins Dpb11 and Slx4 that is under stringent control. Cell cycle-dependent phosphorylation of Slx4 by Cdk1 promotes the Dpb11–Slx4 interaction, while in mitosis, phosphorylation of Mms4 by Polo-like kinase Cdc5 promotes the additional association of Mus81–Mms4 with the complex, thereby promoting JM resolution. Finally, the DNA damage checkpoint counteracts Mus81–Mms4 binding to the Dpb11–Slx4 complex. Thus, Dpb11–Slx4 integrates several cellular inputs and participates in the temporal program for activation of the JM-resolving nuclease Mus81