Aktivitas Antibakteri Dari Bakteri Probiotik Dalam Melawan Propionibacterium acnes Dan Staphylococcus epidermidis

Introduction; Acne vulgaris or commonly called acne, is a disease that is generally suffered by teenagers. Acne is caused by the formation of colonies of pathogenic bacteria such as Propionibacterium acnes and S. epidermidis. Excessive use of antibiotics can lead to bacterial resistance. Therefore, there is a need for other alternatives to the use of antibiotics. Probiotics can be an alternative as an antimicrobial against P. acnes and S. epidermidis bacteria. The aim of this study was to determine the antibacterial activity of probiotic bacteria, namely W. confusa against P. acnes and S. epidermidis. Method; antimicrobial test by agar diffusion method. The positive control used tetracycline antibiotics and the negative control used a blank disk. Results; the presence of antimicrobial activity against P. acnes and S. epidermidis, indicated by the average zone of inhibition in P. acnes of 13.4 mm and S. epidermidis 14 mm. Conclusion; Probiotic bacteria are able to inhibit the growth of pathogenic bacteria that cause acne, namely P. acnes and S. epidermidis.Keywords: Acnes vulgaris, Probiotics, P. acnes, S. epidermidi

ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI PANGAN FERMENTASI CINCALOK SEBAGAI PENGHASIL GAMMA-AMINOBUTYRIC ACID

Isolation and Characterization of Lactic Acid Bacteria from Fermented Food Cincalok as Producer of Gamma-Aminobutyric Acid Cincalok is a fermented food originating from West Kalimantan. This study aimed to obtain lactic acid bacterial isolates (LAB) capable of producing gamma-aminobutyric acid (GABA), to characterize the LAB isolates obtained, and to obtain GABA by the Thin Layer Chromatography (TLC) method. Bacterial growth and GABA production was carried out by adding 5% MSG and without MSG, and measured spectrophotometrically. In this study, 4 LAB bacterial isolates were obtained which were coded CIN-1, CIN-2, CIN-3, and CIN-4. GABA identification of all the LAB isolates using TLC Silica Gel 60 F254 with butanol: acetic acid: distilled water (5: 3: 2) as eluent yielded Rf 0.61 and Rf MSG 0.38. The highest growth was achieved by isolate CIN-3 with an absorbance of 1.488 (at 48 hour) in non-MSG medium, while the addition of 5% MSG resulted in an absorbance of 1.631 (at 42 hour). GABA production was achieved by isolate CIN-3 with 5% MSG treatment with a concentration of 201.472 mM and without MSG with a concentration of 171.195 mM. Cincalok merupakan pangan fermentasi yang berasal dari Kalimantan Barat. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat (BAL) yang mampu menghasilkan gamma-aminobutyric acid (GABA), melakukan karakterisasi isolat BAL yang diperoleh dan dapat diperoleh GABA dengan metode Kromatografi Lapis Tipis (KLT). Penumbuhan bakteri dan produksi GABA dilakukan dengan penambahan MSG 5% dan tanpa MSG, dan diukur menggunakan spektrofotometer. Dalam penelitian ini diperoleh 4 isolat bakteri BAL yang diberi kode CIN-1, CIN-2, CIN-3, dan CIN-4. Identifikasi GABA dari semua isolat BAL tersebut menggunakan KLT Silica Gel 60 F254 dengan eluen butanol: asam asetat: aquades (5: 3: 2), menghasilkan Rf 0,61 dan Rf MSG 0,38. Pertumbuhan tertinggi terjadi pada isolat CIN-3 non MSG dengan absorbansi 1,488 (jam ke-48), sedangkan dengan penambahan MSG 5% menghasilkan absorbansi 1,631 (jam ke-42). Produksi GABA dicapai isolat CIN-3 dengan perlakuan MSG 5% dengan konsentrasi 201.472 mM dan tanpa MSG dengan konsentrasi 171,195 mM

Identifikasi Bakteri Eschericia Coli pada Sumber Air Minum yang Ada di Kantin Sekolah Dasar

Latar belakang: Anak sekolah sangat menyukai konsumsi jajanan minuman yang ada di kantin sekolah. Minuman ini berkemungkinan terkontaminasi oleh bakteri terutama bakteri Coliform. Peraturan Menteri Kesehatan Republik Indonesia Nomor 492/Menkes/Per/IV/2010 menyatakan air minum dinyatakan aman bagi kesehatan apabila memenuhi persyaratan fisika, mikrobiologis, kimiawi dan radioaktif. Pada persyaratan mikrobiologis jumlah Coliform dan Eschericia coli dalam  air  tidak boleh melewati batas yang ditentukan yaitu 0/100 ml sampel air.Tujuan: Untuk mengetahui hubungan sumber air minum dengan kandungan bakteri Eschericia coli dalam air minum di kantin sekolah dasar Kecamatan Tugu SemarangMetode: Metode yang digunakan dalam penelitian ini adalah observasional analitik dengan rancangan cross sectional menggunakan data primer dimana sampel diambil langsung dari seluruh kantin sekolah dasar Kecamatan Tugu SemarangHasil: Total 14 sampel air minum yang didapatkan dari seluruh kantin sekolah dasar Kecamatan Tugu Semarang hanya ada dua jenis sumber air minum yang digunakan, yaitu air sumur dan air minum isi ulang. Ditemukan tiga sampel positif bakteri escherichia coli dari air minum isi ulang. Pada uji statistik Chi square dengan uji alternatif Fisher’s Exact didapatkan hasil p 0,604 > 0,05.Kesimpulan: Tidak terdapat hubungan antara sumber air minum dengan kandungan bakteri Eschericia coli dalam air minum di kantin sekolah dasar Kecamatan Tugu Semarang.Â

IDENTIFICATION OF GABA-PRODUCING LACTIC ACID BACTERIA FROM CINCALOK FERMENTATION BASED ON THE 16S RRNA AND GAD GENES

Objective: The research to identify LAB using 16S rRNA potential as high produce GABA and design primer can amplify that gad gene. Methods: Isolation genomic form LAB, molecular identification based 16S rRNA, design primer use primer3plus, and use application serial cloner to ensure the primer can amplify to target gene. Results: That have been carried out based on the analysis of the 16 S rRNA gene have the highest similarity to Weissella confusa strain JCM 1093 with a similarity of 98.38%, while the results of the analysis of the gad gene with several primers that have been designed are not able to amplify the gad gene owned by W. confusa. Conclusion: The results of the analysis based on the 16S rRNA gene for lactic acid bacteria were obtained by Weissella confusa. However, for the results of identification analysis based on the gad gene, the designed primers were unable to amplify the gad gene in W. confusa