Efecto de brasinoesteroides sintéticos en la inducción de callos de arroz

The present work shows the effect of the synthetic brassinosteroids BIOBRAS 6, ME and BIOBRAS 16 in the induction of callus of rice (Oryza sativa L) of the commercial varieties J-104 and Pokkali. Mature seeds were used from both varieties, using MS medium supplemented with 10-5 mg.l-1 of brassinosteroid alone or combined with 2.4 D (2,4-dichlorophenoxyacetic acid) (2 mg.l-1) and Kin (kinetin) (1 mg.l-1). Both varieties formed compact calluses of nodular appearance and white color, ligth yellow-yellow; achieving in the best treatments 50 and 90 percent of induction in Pokkali and J-104 respectively. Showing a possible citoquinin behavior of the brasinoesteroides studied in the process of calluses induction .The BIOBRAS 6 turned to be a substitute of the Kin in the induction of callus of both varieties while the ME had the same effect in J-104, on the other hand the BIOBRAS 16 was inhibitory.Key words: 2,4-dichlorophenoxyacetic acid, callogenesis,El presente trabajo tuvo como propósito evaluar el efecto de los brasinoesteroides sintéticos BIOBRAS 6, ME y BIOBRAS 16 en la inducción de callos de arroz (Oryza sativa L) de las variedades comerciales J-104 y Pokkali, Para ello se utilizaron semillas maduras de ambas variedades, empleándose el medio de cultivo MS suplementado con 10-5 mg.l-1 de brasinoesteroide, sólo o combinado con 2,4 D (ácido 2,4-diclorofenoxiacético) (2 mg.l-1) y Kin (Kinetina) (1 mg.l-1). Ambas variedades formaron callos compactos de apariencia nodular y color blanco, amarillo-amarillo pálido; alcanzándose en los mejores tratamientos un 90 y 50% de inducción en Pokkali y J-104 respectivamente. El BIOBRAS 6 resultó ser un sustituto de la Kin en la inducción de callos de ambas variedades mientras que el ME tuvo el mismo efecto en J-104, el BIOBRAS 16 fue inhibitorio.Palabras clave: ácido 2,4-diclorofenoxiacético, callogénesis, kinetin

The effect of synthetic brassinosteroid on callus induction of rice

The present work shows the effect of the synthetic brassinosteroids BIOBRAS 6, ME and BIOBRAS 16 in the induction of callus of rice (Oryza sativa L) of the commercial varieties J-104 and Pokkali. Mature seeds were used from both varieties, using MS medium supplemented with 10-5 mg.l-1 of brassinosteroid alone or combined with 2.4 D (2,4-dichlorophenoxyacetic acid) (2 mg.l-1) and Kin (kinetin) (1 mg.l-1). Both varieties formed compact calluses of nodular appearance and white color, ligth yellow-yellow; achieving in the best treatments 50 and 90 percent of induction in Pokkali and J-104 respectively. Showing a possible citoquinin behavior of the brasinoesteroides studied in the process of calluses induction .The BIOBRAS 6 turned to be a substitute of the Kin in the induction of callus of both varieties while the ME had the same effect in J-104, on the other hand the BIOBRAS 16 was inhibitory. Key words: 2,4-dichlorophenoxyacetic acid, callogenesis