Hyperpolarized ^1H NMR employing low γ nucleus for spin polarization storage

The PASADENA (parahydrogen and synthesis allow dramatically enhanced nuclear alignment)(1, 2) and DNP (Dynamic Nuclear Polarization)(3) methods efficiently hyperpolarize biologically relevant nuclei such as 1^H, (31)^P, (13)^C, (15)^N achieving signal enhancement by a factor of ~ 100000 on currently utilized MRI scanners. Recently, many groups have demonstrated the utility of hyperpolarized MR in biological systems using hyperpolarized (13)^C biomarkers with a relatively long spin lattice relaxation time T_1 on the order of tens of seconds.(4-7) Moreover, hyperpolarized (15)^N for biomedical MR has been proposed due to even longer spin lattice relaxations times.(8) An additional increase of up to tens of minutes in the lifetime of hyperpolarized agent in vivo could be achieved by using the singlet states of low gamma (γ) nuclei.(9) However, as NMR receptivity scales as γ^3 for spin 1/2 nuclei, direct NMR detection of low γ nuclei results in a lower signal-to-noise ratio compared to proton detection. While protons are better nuclei for detection, short spin lattice relaxation times prevent direct 1^H hyperpolarized MR in biomedical applications

Metabolic Profiling of Pancreatic Cancer for Early Detection and Determining Therapeutic Efficacy

https://openworks.mdanderson.org/sumexp21/1098/thumbnail.jp

Understanding the effect of stress hormones on ovarian cancer cells

Department of Cancer Systems Imaging Department of Gynecologic Oncology and Reproductive Medicinehttps://openworks.mdanderson.org/sumexp22/1022/thumbnail.jp

Pancreatic Cancer Early Detection trough Hyperpolarized MRI

https://openworks.mdanderson.org/sumexp23/1076/thumbnail.jp

^1H NMR Studies of Nickel(II) Complexes Bound to Oligonucleotides: A Novel Technique for Distinguishing the Binding Locations of Metal Complexes in DNA

The selective paramagnetic relaxation of oligonucleotide proton resonances of d(GTCGAC)_2 and d(GTGCAC)_2 by Ni(phen)_2(L)^(2+) where L = dipyridophenazine (dppz), dipyrido[3,2-d:2‘,3‘-f]quinoxaline (dpq), and phenanthrenequinone (phi) has been examined to obtain structural insight into the noncovalent binding of these metal complexes to DNA. In the oligonucleotide d(GTCGAC)_2, preferential broadening of the G1H8, G4H8, T2H6, and C3H6 proton resonances was observed with Ni(phen)_2(dppz)^(2+), Ni(phen)_2(dpq)^(2+), and Ni(phen)_2(phi)^(2+). In the case of the sequence d(GTGCAC)_2, where the central two bases are juxtaposed from the previous one, preferential broadening was observed instead for the A5H2 proton resonance. Thus, a subtle change in the sequence of the oligonucleotide can cause significant change in the binding location of the metal complex in the oligonucleotide. Owing to comparable changes for all metal complexes and sequences in broadening of the thymine methyl proton resonances, we attribute the switch in preferential broadening to a change in site location within the oligomer rather than to an alteration of groove location. Therefore, even for DNA-binding complexes of low sequence-specificity, distinct variations in binding as a function of sequence are apparent

Early Detection of Pancreatic Cancer by Hyperpolarized MRI

https://openworks.mdanderson.org/sumexp22/1096/thumbnail.jp

Early Detection of Pancreatic Cancer by Hyperpolarization and Artificial Intelligence

https://openworks.mdanderson.org/sumexp21/1099/thumbnail.jp

NMR-based Metabolic Profiling of Pancreatic Cancer and Response to Glutamine Transport Inhibition

https://openworks.mdanderson.org/sumexp21/1227/thumbnail.jp

Distinction Between Pancreatitis and Early-Stage Pancreatic Cancer with HP-MRI

https://openworks.mdanderson.org/sumexp23/1045/thumbnail.jp

Real-Time MRI-Guided Catheter Tracking Using Hyperpolarized Silicon Particles

Visualizing the movement of angiocatheters during endovascular interventions is typically accomplished using x-ray fluoroscopy. There are many potential advantages to developing magnetic resonance imaging-based approaches that will allow three-dimensional imaging of the tissue/vasculature interface while monitoring other physiologically-relevant criteria, without exposing the patient or clinician team to ionizing radiation. Here we introduce a proof-of-concept development of a magnetic resonance imaging-guided catheter tracking method that utilizes hyperpolarized silicon particles. The increased signal of the silicon particles is generated via low-temperature, solid-state dynamic nuclear polarization, and the particles retain their enhanced signal for ?40?minutes—allowing imaging experiments over extended time durations. The particles are affixed to the tip of standard medical-grade catheters and are used to track passage under set distal and temporal points in phantoms and live mouse models. With continued development, this method has the potential to supplement x-ray fluoroscopy and other MRI-guided catheter tracking methods as a zero-background, positive contrast agent that does not require ionizing radiation