BIOGENIC SYNTHESIS OF SILVER NANOPARTICLES AND THEIR SYNERGISTIC EFFECT WITH ANTIBIOTICS: A STUDY AGAINST SALMONELLA SP

Objective: In recent era, medical science has turned to a variety of novel products and technologies to halt the spread of infections. The most promising and effective antimicrobial agents being embraced by modern medical science today is silver. With increasing drug-resistance and growing concern regarding the over-prescribing of antibiotics, there has been a resurgent interest in the use of antimicrobial silver. Unlike antibiotics, silver appears to be immune to resistance. Thus, the conjugation of antibiotic with silver nanoparticles would prevent development of resistance of microbes and increase the antimicrobial property of the antibiotic.Methods: In this manuscript, extracellular biological synthesis of silver nanoparticles was made from Trametes sp. The characterization of the nanoparticles was carried out as well as its antibiotic efficacy was evaluated in addition to the antibiotic combination.Results: Initially the fungal cell filtrate was challenged with 1Mm AgNO3 solution, in a 250 ml Erlenmeyer flask and agitated at 30 °C in dark for 72 h under static conditions at 200 rpm and the silver nanoparticle production was monitored by the change in color and later it was subjected to optical measurements by UV-Vis spectrophotometer. The characters of these silver nanoparticles were further studied by Fourier Transform Infrared (FTIR) Spectroscopy, SEM and TEM studies. The nanoparticles produced during the study period were found to have wider antibacterial property and also it showed the enhanced efficacy in combination with ceftriaxone and ofloxacin against Salmonella sp.Conclusion: The synergistic mode of antibiosis in between nanoparticles synthesized from Trametes sp. and ceftriaxone, ofloxacin was found to be more effective against Salmonella sp. in the current study.Â

Biogenic synthesis of silver nanoparticles from white rot fungi: Their characterization and antibacterial studies

The advanced research and development of silver nanoparticles (AgNPs) is vast due to their incredible applications today. In this work, research was focused on the production, characterization and antibacterial studies of silver nanoparticles (AgNPs). We used an environmentally friendly extracellular biosynthetic method for the production of the AgNPs. The reducing agents used to produce the nanoparticles were from culture filtrate extracts made from two white rot fungi. Synthesis of colloidal AgNPs was monitored by UV-Visible spectroscopy. The UV-Visible spectrum showed a peak between 419 and 421 nm corresponding to the Plasmon absorbance of the AgNPs. The characterization of the AgNPs such as their size and shape was performed by Transmission Electron Microscopy (TEM) techniques which indicated a size range of 15–25 nm. The anti-bacterial activity of AgNPs was investigated by using four Gram-negative and four Gram-positive bacteria. All the bacterial strains are showing good antibacterial activity