Формирование интегрированной системы менеджмента качества на предприятии

Объектом исследования является система менеджмента качества ООО "Томскводоканал". Цель: разработка рекомендаций по созданию и внедрению интегрированной системы менеджмента В процессе исследования проводились: ?Анализ литературных источников по теме ВКР. ?Изучение нормативно-методической базы для разработки документации. ?Анализ систем менеджмента ООО "Томскводоканал". ?Изучение специфики требований стандарта ISO 18000:2007. ?Изучение специфики требований стандарта ISO 14000:2015. -- Создание рекомендации по разработке и внедрению интегрированной системы менеджмента.Object of a research is the quality management system of LLC Tomskvodokanal. Purpose: development of recommendations about creation and introduction of the integrated system of management In the course of the research were carried out: ? The analysis of references on subject VKR. ? Studying of standard and methodical base for development of documentation. ? Analysis of systems of management of LLC Tomskvodokanal. ? Studying of specifics of requirements of ISO 18000:2007. ? Studying of specifics of requirements of ISO 14000:2015. - Creation of the recommendation about development and deployment of the integrated system of management

STAT3 is enriched in nuclear bodies.

Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is involved in a variety of biological functions. It is essential for the signal transduction of interleukin-6 (IL-6) and related cytokines. In response to IL-6 stimulation STAT3 becomes phosphorylated and translocates into the nucleus where it binds to enhancer sequences of target genes. We found that activated STAT3 is enriched in dot-like structures within the nucleus, which we termed STAT3 nuclear bodies. To examine the dynamics of STAT3 nuclear body formation, a fusion protein of STAT3 and yellow fluorescent protein (YFP) was constructed. Studies in living cells have shown that the appearance of STAT3 nuclear bodies is transient, correlating with the timecourse of tyrosine-phosphorylation of STAT3. Furthermore, we show by fluorescence recovery after photobleaching (FRAP) analysis that STAT3 within nuclear bodies consists of a highly mobile and an immobile fraction. Colocalization studies provided evidence that these bodies are accompanied with CREB binding protein (CBP) and acetylated histone H4, which are markers for transcriptionally active chromatin. Moreover, STAT3 nuclear bodies in HepG2 cells are not colocalized with promyelocytic leukemia oncoprotein (PML)-containing bodies; neither is a sumoylation of activated STAT3 detectable. Taken together, our data suggest that STAT3 nuclear bodies are either directly involved in active gene transcription or they serve as reservoirs of activated STAT3