THE ENHANCEMENT OF COLLAGEN SYNTHESIS PROCESS ON DIABETIC WOUND BY MERREMIA MAMMOSA (LOUR.) EXTRACT FRACTION

Objective: This research aimed to evaluate the effect of fractionation of Merremia mammosa Lour. (Mm (Lour.)) extract on diabetic wound healing by observing the collagen synthesis process and to search the most potent fraction. Methods: Wistar rats were divided into five groups (n=5), i.e., K-(negative control), K+(positive control), K1 (ethyl acetate fraction), K2 (water fraction), and K3 (n-hexane fraction). The Mm (Lour.) was extracted with ethanol 70%, then fractionated by using three solvents which have different polarity. The rats were adapted in 7 d, then induced into diabetic by streptozotocin dose 40 mg/kg body weight. The wound was made by Morton excision method. Treatment was given every two days and a skin biopsy was done on day 11. Analysis of collagen density was done by photomicrograph of histopathology preparations in Masson’s trichome stained by using trinocular microscope with 400x magnification in 6 fields of view, then processed by imageJ software and analyzed by appropriate statistic tool. Results: The results of this research showed that fractionation of Mm (Lour.) extract significantly enhanced diabetic wound healing based on macroscopic (percentage of wound healing) and collagen density with p-value<0.05 when compared with negative control, especially the water fraction (p=0.000). The follow-up post hoc analysis showed that there was no­ significant (p=0.989) or there was no meaningful difference in the group of water fraction when compared to positive control. Conclusion: Water fraction is the extract fraction of Mm (Lour.) which has the most significant influence on diabetic wound healing showed by enhancement of collagen synthesis

Syzygium Samarangense Leaves Ointment Enhances Wound Healing Process Of Skin Burn Based On Collagen

Background: The World Health Organization (WHO) estimates the number of death in 2014 caused by burns is 265,000. Burns can caused skin damage as well as other complication problems such as dehydration, infection, and other multiple organ failures. Syzygium samarangense leaves contain flavonoids and saponins that can increase the activation of macrophages and TGF-B which is important to accelerate the process of collagen formation and wound healing process. Objective: Knowing the effect of Syzygium samarangense leaf extract on the healing process of burn based on collagen. Method: This in vivo study use true experimental design. We made burns by placing a coin that already heated in oven at 70ºC for 10 seconds. Rattus Wistar as experimental animals dividedinto 6 groups (n = 4) with details of Group A (normal), B (positive), C (negative). Groups D, E, and F were the groups that given ointment extract topically in doses of 15%, 30%, and 45% each day’s. Termination is done on day 7. Test statistics by using Anova and Kruskal Wallis. Results and discussion: From this research, Syzygium samarangense leaf extract can reduce the wound area (p <0,05) and increase the amount of collagen (p <0,05). Conclusion: Syzygium samarangense leaf extract ointment can accelerate the healing process of burns. Keywords : Burns, Syzygium samarangense Leaves ointment, Saponin, Flavonoid, Collage