Study of the pattern of adverse events following immunization of children in a tertiary care hospital

Background: Success of an immunization programme depends upon awareness regarding usefulness and safety of vaccines. Widespread information on vaccine safety is very essential. Objective of study was to analyze pattern of Adverse Events Following Immunization (AEFI) in children.Methods: Prospective, observational study was carried out at immunization OPD of B. J. Government Medical College and Sassoon General hospital, Pune, where children receiving routine immunization were analyzed & followed up through telephonic survey of parents. Vaccine Adverse Event Reporting System (VAERS) form was used to record AEFI.Results: Total 1206 children, aged 0-6 years, received total of 3179 vaccine doses. 22.71% AEFI were reported. Most common AEFI per 1000 doses of all vaccinations was Fever, seen in less than 9 months of age group (84.67%). DPT vaccine was most common vaccine associated with AEFI and maximum (37.8%) adverse events were seen with 1st dose of DPT.Conclusions: Reporting of AEFI with regular analysis and feedback should be an integral part of immunization programs

Mind over matter: investigation of materialization of intentions

In this article, we present our investigation of materialization of intentions using the input-output data based Six Sigma methodology for problem solving. The investigation is inspired by our respective Gurus, the wisdom of present and past seers, and the works of several scientists. We present two examples of materialization of intentions (change of pH and levitation). The pH example is preliminary requiring additional experimentation. We believe that the evidence presented is very supportive of the hypothesis of materialization of intentions. We hope this paper will contribute towards the unfolding of a Copernican-like revolution which will have profound positive impact on humanity

Genetic diversity and differentiation among populations of the Indian eri silkworm, Samia cynthia ricini, revealed by ISSR markers

Samia cynthia ricini (Lepidoptera:Saturniidae), the Indian eri silkworm, contributes significantly to the production of commercial silk and is widely distributed in the Brahmaputra river valley in North-Eastern India. Due to over exploitation coupled with rapid deforestation, most of the natural populations of S. cynthia ricini are dwindling rapidly and its preservation has become an important goal. Assessment of the genetic structure of each population is a prerequisite for a sustainable conservation program. DNA fingerprinting to detect genetic variation has been used in different insect species not only between populations, but also between individuals within a population. Since, information on the genetic basis of phenotypic variability and genetic diversity within the S. cynthia ricini populations is scanty, inter simple sequence repeat (ISSR) system was used to assess genetic diversity and differentiation among six commercially exploited S. cynthia ricini populations. Twenty ISSR primers produced 87% of inter population variability among the six populations. Genetic distance was lowest between the populations Khanapara (E5) and Mendipathar (E6) (0.0654) and highest between Dhanubhanga (E4) and Titabar (E3) (0.3811). Within population, heterozygosity was higher in Borduar (E2) (0.1093) and lowest in Titabar (E3) (0.0510). Highest gene flow (0.9035) was between E5 and E6 and the lowest (0.2172) was between E3 and E5. Regression analysis showed positive correlation between genetic distance and geographic distance among the populations. The high GST value (0.657) among the populations combined with low gene flow contributes significantly to the genetic differentiation among the S. cynthia ricini populations. Based on genetic diversity, these populations can be considered as different ecotypes and in situ conservation of them is recommended

Cardiovascular disease and the role of oral bacteria

In terms of the pathogenesis of cardiovascular disease (CVD) the focus has traditionally been on dyslipidemia. Over the decades our understanding of the pathogenesis of CVD has increased, and infections, including those caused by oral bacteria, are more likely involved in CVD progression than previously thought. While many studies have now shown an association between periodontal disease and CVD, the mechanisms underpinning this relationship remain unclear. This review gives a brief overview of the host-bacterial interactions in periodontal disease and virulence factors of oral bacteria before discussing the proposed mechanisms by which oral bacterial may facilitate the progression of CVD

Bi-allelic Loss-of-Function CACNA1B Mutations in Progressive Epilepsy-Dyskinesia.

The occurrence of non-epileptic hyperkinetic movements in the context of developmental epileptic encephalopathies is an increasingly recognized phenomenon. Identification of causative mutations provides an important insight into common pathogenic mechanisms that cause both seizures and abnormal motor control. We report bi-allelic loss-of-function CACNA1B variants in six children from three unrelated families whose affected members present with a complex and progressive neurological syndrome. All affected individuals presented with epileptic encephalopathy, severe neurodevelopmental delay (often with regression), and a hyperkinetic movement disorder. Additional neurological features included postnatal microcephaly and hypotonia. Five children died in childhood or adolescence (mean age of death: 9 years), mainly as a result of secondary respiratory complications. CACNA1B encodes the pore-forming subunit of the pre-synaptic neuronal voltage-gated calcium channel Cav2.2/N-type, crucial for SNARE-mediated neurotransmission, particularly in the early postnatal period. Bi-allelic loss-of-function variants in CACNA1B are predicted to cause disruption of Ca2+ influx, leading to impaired synaptic neurotransmission. The resultant effect on neuronal function is likely to be important in the development of involuntary movements and epilepsy. Overall, our findings provide further evidence for the key role of Cav2.2 in normal human neurodevelopment.MAK is funded by an NIHR Research Professorship and receives funding from the Wellcome Trust, Great Ormond Street Children's Hospital Charity, and Rosetrees Trust. E.M. received funding from the Rosetrees Trust (CD-A53) and Great Ormond Street Hospital Children's Charity. K.G. received funding from Temple Street Foundation. A.M. is funded by Great Ormond Street Hospital, the National Institute for Health Research (NIHR), and Biomedical Research Centre. F.L.R. and D.G. are funded by Cambridge Biomedical Research Centre. K.C. and A.S.J. are funded by NIHR Bioresource for Rare Diseases. The DDD Study presents independent research commissioned by the Health Innovation Challenge Fund (grant number HICF-1009-003), a parallel funding partnership between the Wellcome Trust and the Department of Health, and the Wellcome Trust Sanger Institute (grant number WT098051). We acknowledge support from the UK Department of Health via the NIHR comprehensive Biomedical Research Centre award to Guy's and St. Thomas' National Health Service (NHS) Foundation Trust in partnership with King's College London. This research was also supported by the NIHR Great Ormond Street Hospital Biomedical Research Centre. J.H.C. is in receipt of an NIHR Senior Investigator Award. The research team acknowledges the support of the NIHR through the Comprehensive Clinical Research Network. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, Department of Health, or Wellcome Trust. E.R.M. acknowledges support from NIHR Cambridge Biomedical Research Centre, an NIHR Senior Investigator Award, and the University of Cambridge has received salary support in respect of E.R.M. from the NHS in the East of England through the Clinical Academic Reserve. I.E.S. is supported by the National Health and Medical Research Council of Australia (Program Grant and Practitioner Fellowship)

How to pursue pure consciousness? A scientific analysis

A perspective on the development of a science of consciousness is presented. The article begins with a proposed definition of pure consciousness that is followed by an explanation of why anyone might aspire to progress towards it, how one might make progress, what obstacles are likely to be encountered, and what the significance of reaching the destination might be. In the six sigma methodology, major impact factors are the vital few causes that determine systems performance; in the present context, the ability to reach the state of pure consciousness. The paper presents a six sigma analysis of the consciousness effort and identifies a major impact factor, possibly for the first time that will render the pursuit of pure consciousness a bit easier

Towards a science of consciousness: hunt of major impact factors

A perspective on the development of a science of consciousness is presented. The article begins with a proposed definition of pure consciousness that is followed by an explanation of why anyone might aspire to progress towards it, how one might make progress, what obstacles are likely to be encountered, and what the significance of reaching the destination might be. In the six sigma methodology, major impact factors are the vital few causes that determine systems performance; in the present context, the ability to reach the state of pure consciousness. The paper presents a six sigma analysis of the consciousness effort and identifies a major impact factor, possibly for the first time that will render the pursuit of pure consciousness a bit easier

Polyacrylamide gel as a matrix for the delivery of a layer or coat of other molecules

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