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Not AvailableThe genetic diversity of Beauveria bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UPGMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.Not Availabl

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Not AvailableTurmeric rhizome rot is a devastating disease posting the major threat to turmeric cultivation. Plant growthpromoting rhizobacteria (PGPR) Pseudomonas fluorescens strain FP7 was found to exhibit disease reduction and plant growth promotional activity in various crop plants in our previous studies. In this study, efforts were made to evaluate the efficacy of P. fluorescens (FP7) bioformulations against rhizome rot disease in turmeric plants. Among the FP7 bioformulations tested, a combination of rhizome dip and soil drench of FP7 liquid formulation recorded the minimum disease incidence under glasshouse (19.00%) and field conditions (10.18% and 13.29% in the trial I and trial II respectively). Furthermore, the 2D-PAGE analysis was performed to elucidate the molecular responses of tripartite interaction between host-pathogen-bioagent through protein profiling. A total of 12 differentially expressed proteins were identified and mass spectrometry (MS) analysis revealed that proteins such as tryptophan synthase beta subunit-like, phosphoglycerate kinase, cellulose synthase 6 isoform, ATP synthase subunit beta, cysteine-rich peptide, ribosomal protein S3, clathrin assembly protein and disease resistance protein RPP13-like were found to be differentially regulated. The differentially expressed proteins during tripartite interaction might be directly or indirectly involved in disease resistance in turmeric plants. This study provides an initial insight into the molecular mechanism of tripartite interaction between host-pathogen - bioagent in turmeric plantsNot Availabl

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Not AvailableIn this study, we evaluated the efcacy of Bacillus strains for plant growth promotional activities under in vitro and in vivo conditions. The results indicated that Bacillus megaterium BS11 was superior in enhancing the plant growth and yield of rice plants compared to other Bacillus strains. Currently, there is no information available on the molecular mechanism of Rice–B. megaterium interaction for plant growth promotion. Thus, the present study was undertaken to understand the molecular basis of Rice–B. megaterium interaction at the proteome level using the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) approach. Proteomic results revealed that a total of 17 proteins were diferentially expressed in response to BS11 inoculation. The application of BS11 up-regulated most of the identifed proteins involved in plant metabolism, transcription, transporter, signaling, defense, and stress responses, which may underlie the improvement of rice plant growth. Furthermore, the proteomic results were validated at the mRNA level by Quantitative Real-Time PCR (qPCR) analysis. The abundances of proteins and transcripts were positively correlated for all genes except LOC_Os01g68620. Overall, our results suggest that B. megaterium strain BS11 may promote plant growth by improving various metabolism in rice plants.Not Availabl

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Correction: Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers.

[This corrects the article DOI: 10.1371/journal.pone.0211061.]