1 research outputs found
Expression of Activation-Induced Cytidine Deaminase Gene in B Lymphocytes of Patients with Common Variable Immunodeficiency
Objective: Common variable immunodeficiency (CVID) is a heterogeneous
disorder characterized by reduced serum level of IgG, IgA or IgM and
recurrent bacterial infections. Class switch recombination (CSR) as a
critical process in immunoglobulin production is defective in a group
of CVID patients. Activation-induced cytidine deaminase (AID) protein
is an important molecule involving CSR process. The aim of this study
was to investigate the AID gene mRNA production in a group of CVID
patients indicating possible role of this molecule in this disorder.
Methods: Peripheral blood mononuclear cells (PBMC) of 29 CVID patients
and 21 healthy controls were isolated and stimulated by CD40L and IL-4
to induce AID gene expression. After 5 days AID gene mRNA production
was investigated by real time polymerase chain reaction. Findings: AID
gene was expressed in all of the studied patients. However the mean
density of extracted AID mRNA showed higher level in CVID patients
(230.95±103.04 ng/ml) rather than controls (210.00±44.72
ng/ml; P=0.5). CVID cases with lower level of AID had decreased total
level of IgE (P=0.04) and stimulated IgE production (P=0.02); while
cases with increased level of AID presented higher level of IgA
(P=0.04) and numbers of B cells (P=0.02) and autoimmune disease
(P=0.02). Conclusion: Different levels of AID gene expression may have
important roles in dysregulation of immune system and final clinical
presentation in CVID patients. Therefore investigating the expression
of AID gene can help in classifying CVID patients