The Acquisition of Useful Disease Resistance Genes for Hard Red Winter Wheat Improvement

This study was part of a larger pre-breeding effort to develop new parental materials carrying useful genes for disease resistance and adaptation. Firstly, marker-assisted backcrosses were employed to transfer and pyramid combinations of disease resistance genes (Fhb1, Lr34, Lr53, Sr2, Sr26, Sr39, and Sr50) and a reduced height gene (Rht-B1b) into the variety Norstar. Following the third backcross to Norstar, the various backcrossed progenies were inter-mated to derive progenies having combinations of Fhb1 and Rht-B1b plus the targeted leaf and/or stem rust resistance genes. Five NILs (each carrying Fhb1 and Rht-B1b) that differ for the leaf and stem rust resistance genes they possess were recovered. Secondly, a mapping study using the 9K Illumina Infinium iSelect wheat assay was conducted with a F2 mapping population developed by crossing Lr59-25 (0306/2*CSph1b//CSN1AT1B/3/Thatcher) and Superb. Lr59 was mapped 0.5cM distally from the co-segregating SNPs IWA1495, IWA6704, IWA2098 and IWA969 on wheat chromosome arm 6BS.North Dakota Wheat CommissionMinnesota Wheat Research and Promotion CouncilU.S. Wheat and Barley Scab Initiative (USWBSI

Price variation among different brands of anticancer medicines available in hospital pharmacies of Nepal.

Objective:To assess the variation in price among different brands of anticancer medicines available in hospital pharmacies at Nepalese cancer hospitals. Methods:The price of different brands of the same anticancer medicines available in the hospital pharmacies of two cancer hospitals was assessed. Prices of different dosage forms such as a single tablet, capsule and vial were calculated. The difference in the maximum and minimum price of the same drug manufactured by different pharmaceutical industries was determined, and the percentage variation in price was calculated. The prices of medicines (brands) were also compared with the price determined by the government where available. Results:Price variation was assessed for 31 anticancer medicines belonging to six broad categories. Prices were found to vary maximally among the following medicines, each belonging to separate categories: among alkylating agents, the price of temozolomide 100 mg capsule varied 308%; among antimetabolite agents, the price of pemetrexed 500 mg injection varied 134%; among hormonal drugs, the price of letrozole 2.5 mg tablet varied 200%; among antibody class, the price of trastuzumab 440 mg injection varied 73%; among natural products, the price of irinotecan 100 mg injection varied 590%; and among miscellaneous agents, the price of bortezomib 2 mg injection varied 241%. There was a significant difference in the mean MRP of the alkylating agents with the antimetabolites (p-value 0.006) and the monoclonal antibody (p-value <.001). Antimetabolites, natural products, hormonal therapy all had significant mean differences in their MRPs with the monoclonal antibodies. (p-value <.001) and the monoclonal antibodies had a significant mean difference in the MRP with the miscellaneous agents. (p-value <.001). Conclusions:There was a considerable variation in the price of different brands of anticancer medicines available in the Nepalese market. The Government of Nepal has regulated the prices of some medicines, including anticancer medicine. However, it is not enough as prices of the majority of anticancer medicines are still not regulated. Therefore, further strategies are needed to address the variation in the prices of anticancer medicines available in the Nepalese market

Ruthenium catalyzed diol-diene cycloaddition and progress toward total synthesis of andrographolide

In the first chapter, an example of highly exo-selective ruthenium(0) catalyzed transfer hydrogenative cycloaddition is described. These ruthenium catalyzed reactions are analogous to traditional Diels-Alder reactions of cyclohexadiene or norbornadiene but are performed with 1,2-diols instead of the π-unsaturated partners. Novel bridged bicyclic ring systems are accessed from diol, ketol or dione oxidation level with excellent diasteroselectivity. In the second chapter, the ongoing efforts toward the total synthesis of andrographolide, a diterpenoid lactone, is described. The effort supports a modular strategy to use diene intermediate obtained from transformations of tert-hydroxy prenylation product via reaction with π-allyliridium C, O-benzoate complex.Chemistr

Sensors and Systems for Monitoring Mental Fatigue: A systematic review

Mental fatigue is a leading cause of motor vehicle accidents, medical errors, loss of workplace productivity, and student disengagements in e-learning environment. Development of sensors and systems that can reliably track mental fatigue can prevent accidents, reduce errors, and help increase workplace productivity. This review provides a critical summary of theoretical models of mental fatigue, a description of key enabling sensor technologies, and a systematic review of recent studies using biosensor-based systems for tracking mental fatigue in humans. We conducted a systematic search and review of recent literature which focused on detection and tracking of mental fatigue in humans. The search yielded 57 studies (N=1082), majority of which used electroencephalography (EEG) based sensors for tracking mental fatigue. We found that EEG-based sensors can provide a moderate to good sensitivity for fatigue detection. Notably, we found no incremental benefit of using high-density EEG sensors for application in mental fatigue detection. Given the findings, we provide a critical discussion on the integration of wearable EEG and ambient sensors in the context of achieving real-world monitoring. Future work required to advance and adapt the technologies toward widespread deployment of wearable sensors and systems for fatigue monitoring in semi-autonomous and autonomous industries is examined.Comment: 19 Pages, 3 Figure

VARIABILITY AND HERITABILITY ESTIMATE OF 30 RICE LANDRACES OF LAMJUNG AND TANAHUN DISTRICTS, NEPAL

The variability in the rice landraces is the source of variation and offers substantial opportunity for the development of new varieties through the selection. Exploring diversity and variability of landraces is utmost for rice improvement. The objective of the study was to assess diversity indices, magnitude of genetic variability, heritability, genetic advance for the yield, and yield contributing traits. An experiment was carried out in alpha-lattice with 30 landraces, two replications in the Agronomy farm of IAAS, Lamjung Campus, during June-November, 2018. Shannon and Simpson's indices were calculated for 18 qualitative traits. The value of the Shannon and Simpson index ranged 0.15-1.41 and 0.07- 0.75, respectively. Landraces showed significant variations (

The Curation of Genetic Variants: Difficulties and Possible Solutions

The curation of genetic variants from biomedical articles is required for various clinical and research purposes. Nowadays, establishment of variant databases that include overall information about variants is becoming quite popular. These databases have immense utility, serving as a user-friendly information storehouse of variants for information seekers. While manual curation is the gold standard method for curation of variants, it can turn out to be time-consuming on a large scale thus necessitating the need for automation. Curation of variants described in biomedical literature may not be straightforward mainly due to various nomenclature and expression issues. Though current trends in paper writing on variants is inclined to the standard nomenclature such that variants can easily be retrieved, we have a massive store of variants in the literature that are present as non-standard names and the online search engines that are predominantly used may not be capable of finding them. For effective curation of variants, knowledge about the overall process of curation, nature and types of difficulties in curation, and ways to tackle the difficulties during the task are crucial. Only by effective curation, can variants be correctly interpreted. This paper presents the process and difficulties of curation of genetic variants with possible solutions and suggestions from our work experience in the field including literature support. The paper also highlights aspects of interpretation of genetic variants and the importance of writing papers on variants following standard and retrievable methods. © 2012 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China

A Study of Climate Variability and Socioeconomic Impact on Tourism Industry of Nepal

In Nepal, tourism is the second largest industry after agriculture. Furthermore, Nepal offers numerous opportunities for adventurous outdoor recreation, especially in the Himalayas, and attracts touristsfrom all over the World. However, future prosperity of Nepal’s tourism industry might be affected by a changing climate and a socioeconomic environment. A log-level regression model is employed to estimate the impact of climatic and socioeconomic factors on the number of international tourists visiting Nepal. Climatic estimates indicated that an increase in temperature did not have a significant impact on the numbers of international tourist arrival. Results show that the number of international tourists visiting Nepal was positively associated with GDP and population growth, inflation rate, and an exchange rate. These results help policy makers for facilitating growth of tourism industry and its adaptability to climate change in Nepal

Demand and Supply of Forest Products in Bandevi Buffer Zone Community Forest, Chitwan National Park, Nepal

Buffer Zone Community Forestry (BZCF) in the Chitwan National Park (CNP) started with an objective to engage locals and fulfill their resource needs without jeopardizing conservation. This study estimates the forest product demand and supply of fuelwood, fodder, and timber in Bandevi BZCF user group. Data was collected using stratified random sampling and forest inventory using the quadrate method. Results indicated Bandevi BZCF conditions improved since its handover to the community. However, the study estimated a deficit of 26173 cubic meters per year of timber, 3.21 million tonnes per year (Mt/yr) of fodder, and 0.12 Mt/yr of fuelwood. Deficits were fulfilled from agricultural lands outside BZCF and illegal collection from the Bharandavar corridor forest and CNP. BZCF program is a success in improving forest conditions and needs continuation. Policy and programs must focus primarily on livelihood improvement and income generations to reduce the dependency of local people on the forest

Microbial and Physico-Chemical Quality Assessment of Rivers of Kathmandu Valley

Water quality refers to the chemical, physical, biological characteristics of water. It is a measure of condition of water relative to requirement of one or more biotic species and or to any human need or purpose. The main objective of the study is to detect the physio-chemical and microbiological parameters of water sample from the Bagmati river and its tributaries of Kathmandu valley along with antibiotic susceptibility. In physico-chemical parameters, turbidity, temperature, pH, Electrical conductivity, Dissolved Oxygen, Biological Oxygen Demand, Ammonia, Alkalinity, Hardness, Chloride, Phosphate, Iron, Nitrate, Total Dissolved solids, and color were analyzed. Iron and Turbidity was found to be above the World health organization and Nepal Standard guideline in all the samples (100%), while Ammonia was found to be above the WHO guideline in 10(90%) samples. Among 11 samples, 10(90%) showed a low Dissolved oxygen level. Most Probable Number method was followed for counting total load of coliform and fecal coliform. Escherichia coli was isolated from the sample and subjected to Antibiotic susceptibility. Coliform was detected in all the samples and E. coli was identified as highly resistant towards Gentamicin (81.8%) and sensitive towards Chloramphenicol (81.8%). High value of ammonia, turbidity and low value of Dissolved Oxygen in the lower belts of river was due to large inputs of wastewater and organic loads caused by anthropogenic activities. High value of Coliform in all the samples indicates bacterial contamination in river water. The comparative study for the water quality variables in the urban areas showed that the main rivers and its tributaries were equally polluted

Nepalese Commercial Banks: Performance, Non-performing Loans and Corporate Governance

The purpose of this study is to examine the macro-economic, bank specific and corporate governance determinants of non-performing loans in Nepalese banking. In addition to this, the next objective of the study is to analyze the influence of corporate governance on bank performance in Nepal. The study is to investigate the above objectives in order to improve the means by which commercial banks in Nepal manage their non-performing loans and performance. This will provide guidance for the regulatory bodies in safeguarding the stability and integrity of the Nepalese financial system