4 research outputs found
Influence of cadmium chloride on the frequency of micronuclei in da and AO rats
Heavy metal cadmium (Cd), a well-known environmental hazard, exerts a number of toxic and genotoxic effects. Experimental animals, Albino Oxford (AO) and Dark August (DA) rat strains, were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight, while the control animals received equal volume of sterile phosphate buffered saline. In this investigation individuals of both sexes aged 3, 6 and 12 month were used. Frequency of micronuclei formation was evaluated in polychromatic erythrocytes (PCEs), 24h hours after treatment. The results showed that that cadmium-cloride (CdCl2) exibits the genotoxic effects causing an increase of the frequency of micronuclei depending on concentration, sex, age and strain
The effect of different acute concentrations of cadmium chloride on the frequency of micronuclei in AO rats
Cadmium (Cd) is highly toxic heavy metal which may cause severe biological
effects in vivo and in vitro. In this study, an evaluation of the acute Cd
ability to trigger micronuclei (MNi) formation was carried out on 3-monthold
male and female Albino Oxford (AO) rats using micronucleus (MN) test.
Experimental animals were treated intraperitoneally with three different
concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of
body weight. Control animals received equal volume of sterile phosphate
buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration
caused a highly statistically significant (P < 0.001) increase in MNi
formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a
clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used
(0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical
significance. Sex differences in MNi production in bone marrow PCEs after
acute exposure to different experimental concentrations of CdCl2 were not
observed in our study. Our results indicate the ability of CdCl2 to exerts
genotoxic effects in bone marrow of AO rats, and complement previous data on
the genotoxicity of this important environmental contaminant, burdening the
body from different sources - major being industrial exposure, food and
cigarette smoking. [Projekat Ministarstva nauke Republike Srbije, br. III46002
Induction of micronuclei by cadmium chloride in AO rats depends on age and sex
Cadmium (Cd) is one of the most toxic industrial and metal in the environment
which may cause severe genotoxic effects. The aim of the work was an
evaluation of genotoxic effects of CdCl2 in genetically pure Albino Oxford
(AO) rats, depending on sex, age and dosage. Experimental animals were
treated intraperitoneally with three different concentrations of CdCl2: 0.5,
1, and 2 mg/kg of CdCl2, while the control animals received equal volume of
sterile phosphate buffered saline. The individuals of both sexes were treated
at the age of 3, 6 and 12 month. Frequency of micronuclei formation was
evaluated in polychromatic erythrocytes (PCEs), 24h hours after the
treatment. The results showed that CdCl2 caused a concentration-dependent
increase of micronucleus frequency. The most significant differences were
found between ages of 3/12 and 6/12 months at 0.5 and 1.0 mg CdCl2
concentrations. Namely, 3 month old males had higher frequency of MNi in
comparison to 12 month old males, whereas in females it was the opposite.
Likewise, 6 months old males exbibited greater sensistivity to CdCl2 in
comparison to 12 month old rats, and in the females it was the opposite. Sex
differences were further confirmed as slightly stronger genotoxic effects in
12 months old females treated with 0.5 and 1 mg/kg of CdCl2. Therefore, the
genotoxic effects of cadmium in AO rats depend on concentration, age and sex.
[Projekat Ministarstva nauke Republike Srbije, br. III46002 i br. OI173012
Influence of cadmium chloride on the frequency of micronuclei in DA and AO rats
Heavy metal cadmium (Cd), a well-known environmental hazard, exerts a number
of toxic and genotoxic effects. Experimental animals, Albino Oxford (AO) and
Dark August (DA) rat strains, were treated intraperitoneally with three
different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2
per kg of body weight, while the control animals received equal volume of
sterile phosphate buffered saline. In this investigation individuals of both
sexes aged 3, 6 and 12 month were used. Frequency of micronuclei formation
was evaluated in polychromatic erythrocytes (PCEs), 24h hours after
treatment. The results showed that that cadmium-cloride (CdCl2) exibits the
genotoxic effects causing an increase of the frequency of micronuclei
depending on concentration, sex, age and strain. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III46002 and Grant no. OI173012