Screening of Chlamydia trachomatis urogenital infections among the male and female population of the Republic of Macedonia

Noninvasive urine screening for Chlamydia trachomatis infections offers a valuable public health tool, that could be of vast importance in Chlamydia control programs. The goal was to determine the prevalence of C. trachomatis infections among a sexually active population, to define the epidemiological factors associated with it, and to develop potential selective screening strategies among asymptomatic individuals in the Republic of Macedonia, using a highly sensitive and specific DNA amplification method for C. trachomatis. A total of 1435 urine samples, divided into two main groups: asymptomatic individuals (n = 1210) and symptomatic patients (n = 225), were tested. Samples from the asymptomatic group were collected during routine screening programs, while the symptomatic group consisted of patients with symptoms of urogenital tract infection, attending sexually transmitted diseases (STD) clinics. The presence of C. trachomatis was determined using commercial AMPLICOR C. trachomatis Assay (Roche Diagnostic Systems, Inc., Branchburg, NJ, USA). The prevalence of C. trachomatis infections among different groups was: recruits 0%, soldiers 0.4%, policemen 3.5%, clerks 4.6%, pregnant women 4%, and students 4.4%. The average prevalence for both groups (asymptomatic and symptomatic) was 2.3%[95% confidence interval (CI): 1.5-3.1%]. The average prevalence for the asymptomatic group was 1.6% (95% CI: 0.8-2.4%), while the average prevalence for the symptomatic group was 6.2% (95% CI: 3.1-9.3%) which were significantly different (P = 0.00003). Testing first void urine specimens by AMPLICOR C. trachomatis assay is a highly sensitive and specific method for diagnosing C. trachomatis infections in men and women. This method provides health care workers and public health officials with a new molecular amplification assay that uses noninvasive urine specimens for population-based screening purposes. The prevalence of C. trachomatis was relatively low among asymptomatic individuals. However, selective screening strategies are highly recommended for testing the student population in the Republic of Macedonia. Journal of the European Academy of Dermatology and Venereology 08/2005; 19(4):427-30. • 2.69 Impact Facto

An Algorithm for Calculating Multi-State Network Reliability with Arbitrary Capacities of the Links

Abstract: The problem that we regard in this paper is known as the multi-state two-terminal reliability computation, and we consider how the concept of the minimal path and cut vectors is used in modeling the reliability of these types of systems. The main focus is to develop an algo- rithm for obtaining minimal path vectors for multi-state two-terminal network. The proposed algo- rithm differs from the other known algorithms for this problem, because it does not request any restrictions for the values of the capacities of the links. Some examples to illustrate the algo- rithm are included. Keywords: Reliability, multi-state systems, network reliability, minimal path vector

Computer assisted diagnosis of benign bone tumours

The aim of this study is to determine the correlation between computer-assisted diagnosis(CAD) of benign bone tumours (BBT) and their histological type. Diagnosis and treatment of benign bone tumours (BBT) is a multidisciplinary task. Teams of diverse subspecialists are involved in the process. Good quality plain X-rays may be most helpful in 9 of 10 cases. Bone scan, CT and MRI are additionally needed for the diagnosis, staging and decision making on the management of BBT. The diagnosis of histological type can be done exclusively by a patohistyologist

Extending Robot Therapy for Children with Autism Using Mobile and Web Application

Robot treatments for children with autism have proven to be successful and effective. However, the resources needed for the treatments do not always meet the needs of the children. We overcame the lack of equipment and staff by extending the concept of robot therapy using a web and mobile application. This application enables greater availability and personification of the therapy itself. Its use in the majority of respondents contributes to improving their condition. This approach increases the flexibility of the therapy itself and makes it more accessible, enabling the patients to progress more rapidly. Although the robotic treatment presented in this paper is specific to children with autism, this approach can be generalized and applied to other areas where there are similar types of therapies

HPV E6/E7 mRNA Versus HPV DNA Biomarker in Cervical Cancer Screening of a Group of Macedonian Women

High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance of HPV E6/E7 mRNA as an early biomarker versus HPV DNA detection and cytology in triage of woman for cervical cancer. The study group consists of 413 women: 258 NILM, 26 ASC-US, 81 LSIL, 41 HSIL, and 7 unsatisfactory cytology. HPV4AACE screening, real-time multiplex PCR and MY09/11 consensus PCR primers methods were used for the HPV DNA detection. The real-time multiplex nucleic acid sequence-based assay (NucliSENS EasyQ HPV assay) was used for HPV E6/E7 mRNA detection of the five most common high risk HPV types in cervical cancer (16, 18, 31, 33, and 45). The results show that HPV E6/E7 mRNA testing had a higher specificity 50% (95% CI 32–67) and positive predictive value (PPV) 62% (95% CI 46–76) for CIN2+ compared to HPV DNA testing that had specificity of 18% (95% CI 7–37) and PPV 52% (95% CI 39–76) respectively. The higher specificity and PPV of HPV E6/E7 mRNA testing are valuable in predicting insignificant HPV DNA infection among cases with borderline cytological finding. It can help in avoiding aggressive procedures (biopsies and over-referral of transient HPV infections) as well as lowering patient's anxiety and follow up period