School in Italy: a safe place for children and adolescents

Background: During the first SARS-CoV-2 pandemic phase, the sudden closure of schools was one of the main measures to minimize the spread of the virus. In the second phase, several safety procedures were implemented to avoid school closure. To evaluate if the school is a safe place, students and staff of two school complexes of Rome were monitored to evaluate the efficacy of prevention measures inside the school buildings. Methods: Oral secretions specimens were collected from 1262 subjects for a total of 3431 samples, collected over a 3 months period. Detection of Coronavirus SARS-CoV-2 was performed by real-time PCR. Target genes were represented by E gene, RdRP/S gene and N gene. Results: Among the 3431 samples analyzed, just 16 sample resulted as positive or low positive: 1 sample in the first month, 12 samples in the second month and 3 in the third month. In each period of evaluation, all positive children attended different classes. Conclusions: Even if the school has the potential for spreading viruses, our preliminary results show the efficacy of the implementations undertaken in this setting to minimize virus diffusion. Our evidence suggests that school does not act as an amplifier for transmission of SARS-CoV-2 and can be really considered a safe place for students

Gadd45b Acts as Neuroprotective Effector in Global Ischemia-Induced Neuronal Death

Purpose Transient global ischemia arising in human due to cardiac arrest causes selective, delayed neuronal death in hippocampal CA1 and cognitive impairment. Growth arrest and DNA-damage-inducible protein 45 beta (Gadd45b) is a wellknown molecule in both DNA damage-related pathogenesis and therapies. Emerging evidence suggests that Gadd45b is an anti-apoptotic factor in nonneuronal cells and is an intrinsic neuroprotective molecule in neurons. However, the mechanism of Gadd45b pathway is not fully examined in neurodegeneration associated with global ischemia. Methods Rats were subjected to transient global ischemia by the 4-vessel occlusion or sham operation. The animals were sacrificed at 24 hours, 48 hours, and 7 days after ischemia. The hippocampal CA1 was microdissected and processed to examine mRNA and protein level. To assess neuronal death, tissue sections were cut and processed for Fluoro-Jade and Nissl staining. Results Here we show that ischemic insults increase abundance of Gadd45b and brain-derived neurotrophic factor, a known target of Gadd45 mediated demethylation, in selectively-vulnerable hippocampal CA1 neurons. We further show that knockdown of Gadd45b increases abundance of a pro-apoptotic Bcl-2 family member Bax while decreasing the antiapoptotic protein Bcl-2, which together promote neuronal death. Conclusions These findings document a protective role of Gadd45b against neuronal insults associated with global ischemia and identify Gadd45b as a potential therapeutic target for the amelioration of hippocampal neurodegeneration

Design and analysis of single-event tolerant slave latches for enhanced scan delay testing

The last few years have seen the development and fabrication of nanoscale circuits at high density and low power. Following a single-event upset (SEU), so-called soft errors due to internal and externally induced phenomena (such as α-particles and cosmic rays in adverse environments) have been reported during system operation; this is especially deleterious for storage elements such as flip-flops. To reduce the impact of a soft error on flip-flops, hardening techniques have been utilized. This paper proposes two new slave latches for improving the SEU tolerance of a flip-flop in scan delay testing. The two proposed slave latches utilize additional circuitry to increase the critical charge of the flip-flop compared to designs found in the technical literature. When used in a flip-flop, the first (second) latch design achieves a 5.6 (2.4) times larger critical charge with 11% (4%) delay and 16% (9%) power consumption overhead at 32-nm feature size, as compared with the best design found in the technical literature. Moreover, it is shown that the proposed slave latches have also superior performance in the presence of a single event with a multiple-node upset

QCA Circuits for Robust Coplanar Crossing

In this paper, different circuits of Quantum-dot Cellular Automata (QCA) are proposed for the so-called coplanar crossing. Coplanar crossing is one of the most interesting features of QCA because it allows for mono-layered interconnected circuits, whereas CMOS technology needs different levels of metalization. However, the characteristics of the coplanar crossing make it prone to malfunction due to thermal noise or defects. The proposed circuits exploit the majority voting properties of QCA to allow a robust crossing of wires on the Cartesian plane. This is accomplished using enlarged lines and voting. A Bayesian Network (BN) based simulator is utilized for evaluation; results are provided to assess robustness in the presence of cell defects and thermal effects. The BN simulator provides fast and reliable computation of the signal polarization versus normalized temperature. Simulation of the wire crossing circuits at different operating temperatures is provided with respect to defects and a quantitative metric for performance under temperature variations is proposed and assessed. I

Novel memory designs for qca implementation

Abstract — Quantum-dot Cellular Automata (QCA) provides a new functional paradigm for information processing and communication. The main feature of this technology is the so-called processing-in-wire mechanism by which data movement and manipulation are strictly integrated. In this context, the design of memory devices is particularly challenging and interesting becuse the conventional storage arrangements applicable to CMOS based memories cannot be applied and innovative approaches must be used. This paper analyzes state-of-the art designs for QCA memories and proposes three new architectures that improve over past approaches different figures of merit. I

Novel designs for thermally robust coplanar crossing

In this paper, different circuit arrangements of Quantumdot Cellular Automata (QCA) are proposed for the so-called coplanar crossing. These arrangements exploit the majority voting properties of QCA to allow a robust crossing of wires on the Cartesian plane. This is accomplished using enlarged lines and voting. Using a Bayesian Network (BN) based simulator, new results are provided to evaluate the robustness to so-called kink of these arrangements to thermal variations. The BN simulator provides fast and reliable computation of the signal polarization versus normalized temperature. It is shown that by modifying the layout, a higher polarization level can be achieved in the routed signal by utilizing the proposed QCA arrangements. 1

Modeling Open Defects in Nanometric Scale CMOS

Open defects are extremely common in CMOS circuits. They can either be a partial or complete breaking of an input line. The complete breaking of the line is referred to as strong or full open defect. Until few years ago, a full open defect on any interconnecting line has been considered as floating. In nanometric CMOS technology, in which gate leakage currents are not negligible, full open defect lines cannot be considered to be electrically isolated. The final value of the node is independent of the initial state of the node and totally depends on the topological characteristics of the gate. Experimental evidence of the behavior of all basic gates at 90nm, 64nm and 32nm is provided; this shows a decrease in the drain current to gate leakage current ratio, in the technology scaling. The effect of full opens at the gates has also been tested by varying the PVT conditions. These variations provide a range of variation for the full open input voltage and gate leakage current. The effect of full opens on various circuits like the full adder has also been documented at various nanometric levels. 1