17 research outputs found
Attachment of Nucleosides to SolidâPhase Supports
Specific stepâbyâstep instructions are given for coupling nucleosides to LCAAâCPG supports (supports consisting of a longâchain alkylamine linked to controlledâpore glass). Protocols are given for a succinic acid linker and a hydroquinoneâO,Oâ˛âdiacetic acid linker. The former is the most widely used linker arm, and the starting materials are widely available. The latter offers greater compatibility with baseâsensitive sequence modifications and great synthetic throughput because it can be cleaved under milder and faster conditions. Additional guidelines are given for selecting a linker arm and coupling protocol. Almost any application requiring synthetic oligonucletodies can be satisfied using one of these linker arms.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143722/1/cpnc0302.pd
SolidâPhase Supports for Oligonucleotide Synthesis
This unit begins with a discussion of the advantages and disadvantages of oligonucleotide synthesis using solid supports. The physical and chemical properties of solidâphase supports are discussed in terms of their suitability for oligonucleotide synthesis. In addition, the unit outlines the properties of linkers used for transient or permanent attachment of properly protected nucleosides to the derivatized support, as well as strategies for coupling nucleosides to linkers and conditions for the release of synthetic oligonucleotides from specific supports.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143613/1/cpnc0301.pd
Strategies for Oligoribonucleotide Synthesis According to the Phosphoramidite Method
Advances in oligoribonucleotide synthesis have lagged behind those in oligodeoxyribonucleotide synthesis because of the difficulty in identifying orthogonal protecting groups for the 2â˛â and 5â˛âhydroxyls. Adaptation of the phosphoramidite method for DNA synthesis to RNA synthesis has greatly improved our understanding of RNA. It allows siteâspecific introduction of modified nucleosides to any position in an RNA molecule, as well as introduction of variations at multiple sites in the molecule. This overview discusses issues that are relevant to RNA synthesis by the phosphoramidite approach, including supports used, activation of the ribonucleoside phosphoramidites, and protection of the nucleobase, phosphate, and 2â˛â and 5â˛âhydroxyls.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143793/1/cpnc0305.pd