The hydrogenation of metals upon interaction with water

Hydrogen evolution at 600 deg and 5 x 10 to the 7th power - 10 to the 6th power torr from AVOOO Al samples, which were pickled in 10 percent NaOH, is discussed. An H evolution kinetic equation is derived for samples of equal vol. and different surfaces (5 and 20 sq cm). The values of the H evolution coefficient K indicated an agreement with considered H diffusion mechanism through an oxide layer. The activation energy for the H evolution process, obtained from the K-temp. relation, was 13,000 2000 cal/g-atom

Translocation, switching and gating: potential roles for ATP in long-range communication on DNA by Type III restriction endonucleases

To cleave DNA, the Type III RM (restriction–modification) enzymes must communicate the relative orientation of two recognition sequences, which may be separated by many thousands of base pairs. This long-range interaction requires ATP hydrolysis by a helicase domain, and both active (DNA translocation) and passive (DNA sliding) modes of motion along DNA have been proposed. Potential roles for ATP binding and hydrolysis by the helicase domains are discussed, with a focus on bipartite ATPases that act as molecular switches

Влияние элементов технологии возделывания на фитосанитарное состояние посевов и урожайность зерновых культур

The study aims to assess the impact of the main tillage system, predecessors and mineral fertilizers on the phytosanitary condition of crops and grain yield in the grain fallow crop rotation. The authors researched the educational and experimental farm «Minderlinskoye» FSBEI HE Krasnoyarsk State Agrarian University, located in the Krasnoyarsk forest-steppe conditions. It was found that the application of the herbicide tank mixture (preparations Puma Super 100 + Sekator Turbo) has a significant positive effect on the regulatory impact on the weed component of agrophytocenosis. The authors established the effect in variants with traditional moldboard main tillage (plowing at 20–22 cm) and without it in fertilized and unfertilized backgrounds. The highest technical efficiency of the herbicides applied was observed in the moldboard treatment variant (86–94%) compared to thenon-treatment variant. The authors revealed a decrease in the damage to the underground organs of spring wheatwhen placed after green manure rapeseed fallow in options with moldboard plowing by 20–22 cm and without basic tillage compared to such a predecessor as corn. The index of root rot development increases by 23.2% (plowing, fertilized background) and 37.5% (plowing, unfertilized background) when barley is placed as a second grain crop. These figures are 21.0% and 33.2% for no-tillage, which ultimately affects the yield of grain crops. The highest biological yield of spring wheat grain was obtained in the variant with moldboard plowing after green manure rapeseed fallow. The grain yield of spring wheat was higher in the variant without basic tillage on the fertilized background when placing crops of this crop after corn.Цель исследований – оценить влияние системы основной обработки почвы, предшественников и минеральных удобрений на фитосанитарное состояние посевов и урожайность зерновых культур в зернопаропропашном севообороте. Исследования проведены в учебно-опытном хозяйстве «Миндерлинское» ФГБОУ ВО Красноярский ГАУ, расположенном в условиях Красноярской лесостепи. Установлено, что применение баковой смеси гербицидов (Пума Супер 100 + Секатор Турбо) в вариантах с традиционной отвальной основной обработки почвы (вспашка на 20–22 см) и без ее проведения на удобренном и неудобренном фонах оказывает значительный положительный эффект регулирующего воздействия на сорный компонент агрофитоценоза. Самая высокая техническая эффективность применяемых гербицидов отмечена в варианте с отвальной обработкой (86–94 %) по сравнению с вариантом без ее проведения. Выявлено снижение заболеваемости подземных органов яровой пшеницы при размещении после сидерального рапсового пара в вариантах с отвальной вспашкой на 20–22 см и без проведения основной обработки почвы по сравнению с таким предшественником, как кукуруза. При размещении ячменя в качестве повторной зерновой культуры индекс развития корневых гнилей возрастает на 23,2 (вспашка, удобренный фон) и 37,5 % (вспашка, неудобренный фон). При отказе от обработки почвы эти цифры составляют соответственно 21,0 и 33,2 %, что в конечном счете сказывается на урожайности зерновых культур. Наибольшая биологическая урожайность зерна яровой пшеницы получена в варианте с отвальной вспашкой после сидерального рапсового пара. В варианте без основной обработки почвы урожайность зерна яровой пшеницы была выше на удобренном фоне при размещении посевов этой культуры после кукурузы

A PLAC8-containing protein from an endomycorrhizal fungus confers cadmium resistance to yeast cells by interacting with Mlh3p

Cadmium is a genotoxic pollutant known to target proteins that are involved in DNA repair and in antioxidant defence, altering their functions and ultimately causing mutagenic and carcinogenic effects. We have identified a PLAC8 domain-containing protein, named OmFCR, by a yeast functional screen aimed at identifying genes involved in cadmium resistance in the endomycorrhizal fungus Oidiodendron maius. OmFCR shows a remarkable specificity in mediating cadmium resistance. Both its function and its nuclear localization in yeast strictly depend on the interaction with Mlh3p, a subunit of the mismatch repair (MMR) system. Although proteins belonging to the PLAC8 family are widespread in eukaryotes, they are poorly characterized and their biological role still remains elusive. Our work represents the first report about the potential role of a PLAC8 protein in physically coupling DNA lesion recognition by the MMR system to appropriate effectors that affect cell cycle checkpoint pathways. On the basis of cell survival assays and yeast growth curves, we hypothesize that, upon cadmium exposure, OmFCR might promote a higher rate of cell division as compared to control cells

The C-Terminal Domain of the MutL Homolog from Neisseria gonorrhoeae Forms an Inverted Homodimer

The mismatch repair (MMR) pathway serves to maintain the integrity of the genome by removing mispaired bases from the newly synthesized strand. In E. coli, MutS, MutL and MutH coordinate to discriminate the daughter strand through a mechanism involving lack of methylation on the new strand. This facilitates the creation of a nick by MutH in the daughter strand to initiate mismatch repair. Many bacteria and eukaryotes, including humans, do not possess a homolog of MutH. Although the exact strategy for strand discrimination in these organisms is yet to be ascertained, the required nicking endonuclease activity is resident in the C-terminal domain of MutL. This activity is dependent on the integrity of a conserved metal binding motif. Unlike their eukaryotic counterparts, MutL in bacteria like Neisseria exist in the form of a homodimer. Even though this homodimer would possess two active sites, it still acts a nicking endonuclease. Here, we present the crystal structure of the C-terminal domain (CTD) of the MutL homolog of Neisseria gonorrhoeae (NgoL) determined to a resolution of 2.4 Å. The structure shows that the metal binding motif exists in a helical configuration and that four of the six conserved motifs in the MutL family, including the metal binding site, localize together to form a composite active site. NgoL-CTD exists in the form of an elongated inverted homodimer stabilized by a hydrophobic interface rich in leucines. The inverted arrangement places the two composite active sites in each subunit on opposite lateral sides of the homodimer. Such an arrangement raises the possibility that one of the active sites is occluded due to interaction of NgoL with other protein factors involved in MMR. The presentation of only one active site to substrate DNA will ensure that nicking of only one strand occurs to prevent inadvertent and deleterious double stranded cleavage

Madame de Genlis dans la littérature russe du XIXe siècle: Pouchkine, Léon Tolstoï et autres

Contains fulltext : 120946pub.pdf (publisher's version ) (Open Access)18 p

Trois lettres et une notice de Madame de Genlis conservées dans les bibliothèques russes

Contains fulltext : 121280pub.pdf (publisher's version ) (Open Access)7 p

Tolstoy and А.S. Pushkin: Comparative history of private book collections

This paper considers the origin, formation, and destiny of the book collection in Yasnaya Polyana. Its history begins from the time of Nikolai Semenovich Volkonskii (1753–1821). A great contribution to this book collection was made not only by his grandson, Leo Nikolaevich Tolstoy (1828–1910), but also by the generation of contemporaries of the writer. Special attention has been paid to the works of Alexander Sergeevich Pushkin (1799–1837) and the literature about him. The work is based on the receptive analysis of the source material, because this method allows to involve numerous memories. In particular, we have speculated about L.N. Tolstoy's attitude to A.S. Pushkin's works, as well as about the role played by the “sun of Russian poetry” in his literary work and in the process of educating children based on the memoirs of the following persons: L.N. Tolstoy's sons, Sergey (1866–1933) and Ilya (1866–1933); his family doctor, Dushan Petrovich Makovitskii (1866–1921); Grigory Petrovich Danilevskii (1829–1890), who was the first to develop an idea of the library in Yasnaya Polyana; Alexey Evgen'evich Gruzinskii (1858–1930), a professor of Moscow University, who studied the state of books in 1912 with the permission of Sofia Tolstoya (1844–1919), the widow of the writer et al