Characterization of Genes Involved in Chromatic Acclimation in the Cyanobacterium Synechococcus sp. A 15-62

Synechococcus, a genus of photosynthetic cyanobacteria, is the second most abundant oxygenic microorganism in the marine environment that contributes significantly to the ocean’s primary productivity (Humily et al. 2013; Shukla et al. 2012). They are capable of utilizing available light of different wavelengths in the visible spectrum to perform photosynthesis and fix carbon dioxide and thus inhabit a wide range of light niches in the ocean along horizontal (coast vs offshore) and vertical gradients (depth) (Humily et al. 2013). A gene encoding a putative lyase isomerase, mpeQ, is present in phycoerythrin-II encoding operon that is expressed constitutively and a gene encoding putative lyase, mpeW, is present in CA-4 genomic island whose expression is regulated by ambient light color were identified and characterized in Synechococcus sp. A15- 62, a strain having a blue light specialist phenotype in its basal state. The amino acid sequence of the proteins encoded by mpeW and mpeQ are similar to other characterized lyases and these genes are conserved in cyanobacteria strains containing the CA4-B genomic island, which controls CA4 (Humily et al. 2013). The MpeW and MpeQ proteins were produced in E. coli and co-expressed with recombinant HT-MpeA and phycoerythrobilin (PEB) synthesis machinery. Site directed mutants of the HT-MpeA protein (Cys75Ala, Cys83Ala, Cys140Ala) were used to investigate the site for bilin attachment. The recombinant protein co-expression experiments of MpeQ and MpeW demonstrated that MpeQ attaches phycoerythrobilin (PEB) to cysteine-83 site on a-phycoerythrin II and isomerizes it to phycourobilin (PUB) and MpeW attaches phycoerythrobilin (PEB) to the same site

Motor nerve conduction study parameters in healthy individuals: effect of limb dominance

Background: Nerve conduction study (NCS) is useful for evaluation of nerve, muscle, and/or neuromuscular function. Neurophysiologist interprets NCS with consideration of various anthropometric and technical parameters viz. age, gender, height, temperature etc. apart from the underlying pathology. Fewer studies have reported the effect of limb dominance on NCS. Moreover, the findings are controversial. Therefore, author aimed to investigate the effect of limb dominance on motor nerve conduction study parameters.Methods: This cross-sectional comparative study included sixty healthy individuals (44 right and 16 left handed) of either sex with age 18 to 30 years. The NCS parameters of median and ulnar nerves were assessed by stimulating it and recording from the muscle and skin overlying the nerve respectively using Digital Nihon Kohden machine. The obtained data were analyzed using independent sample t-test.Results: Right ulnar nerve onset latency was significantly longer in left-handed individuals (1.85±0.508 ms vs 1.62±0.195 ms, p=0.012). The left ulnar nerve F wave minimum latency (25.88±0.74 ms vs 24.46±2.64 ms, p=0.002) was significantly longer in left-handed individuals. Likewise, right ulnar nerve distal latency (2.45±0.76 ms vs 2.14±0.39 ms, p=0.044), and right ulnar nerve F wave minimum (25.9±1.21 ms vs 24.85 ms±1.74, p=0.030) were significantly high in left-handed individuals.Conclusions: NCS parameters in terms of latencies were longer in left-handed individuals. Therefore, limb dominance seems to be an important factor one should pay attention during bilateral comparison of obtained data in neurophysiological reporting of referred cases

Orthopaedic services during COVID-19 lockdown at Patan Hospital, Nepal

Introduction: The COVID-19 pandemic has paralyzed the world, including elective health care services. To prevent the spread of infection, most countries have gone into lockdown and adjustments have been made to provide urgent medical care, including Orthopaedic services. In accordance with the guidelines from worst affected countries and neighboring India, Patan Hospital followed instructions from Ministry of Health and Population to provide only urgent and semi-urgent Orthopaedic services. This study aims to audit the patient profile during lockdown so as to have a clearer picture, which will enable us to be prepared for similar epidemic in the future. Method: All patients admitted to the Orthopaedic ward of Patan hospital from 24 March to 27 April 2020, during the lockdown, were included. Clinical profile, including cause of admission, management, hospital stay were descriptively analyzed. Ethical approval was obtained. Result: Out of 44 admissions, there were male 27 and female 17. Trauma cases were 38, and 18 were in age group 20-26 years. Admission due to infections were four. Conservative management were done in seven while 33 were treated surgically, out of which 30 accounted for trauma. Average 6.14 days hospital stay, range 1-22 days. Conclusion: Trauma comprised of major bulk of patients seeking urgent Orthopaedic care. Hospital needs to be prepared with necessary measures to ensure safety of health care workers and yet provide urgent Orthopaedic services. Keyword: COVID-19, lockdown, orthopaedic

Should all pregnant women take calcium supplements in Nepal? GRADE evidence to policy assessment

Background The WHO recommends oral calcium supplementation (1.5–2.0 g) in pregnant women to reduce the risk of pre-eclampsia living in areas with low dietary calcium intake. Although maternal mortality is high in Nepal and eclampsia causes at least 20% of maternal deaths, implementing WHO recommendations would be a major undertaking. Objective This review aimed to assess whether the current evidence supports the blanket supplementation of calcium to prevent pre-eclampsia among pregnant women in Nepal. Methods We used a structured approach to appraise the evidence for calcium supplementation in Nepal. We identified what may influence the impact of calcium supplementation in Nepal and conducted a situation analysis in the country covering maternal mortality, pre-eclampsia occurrence, and existing government policy provisions for supplementation. We also consulted with experts and government officials to explore their perspectives and experience on supplementation. We then used AMSTAR (A MeaSurement Tool to Assess Systematic Reviews) to appraise the Cochrane Systematic Review of calcium supplementation. Finally, we used these data in a GRADE (Grading of Recommendations Assessment, Development and Evaluation)–Evidence to Decision framework to reach a policy recommendation. Results Our assessment of the Cochrane Review showed that the recommendation made by the WHO is based on weak evidence and trial findings that are not consistent between studies. The Cochrane Review found low certainty of the evidence for benefit (reduction in pre-eclampsia and maternal mortality). Conversely, there is a high certainty of the evidence of undesirable effects (HELLP [haemolysis, elevated liver enzymes and low platelets] syndrome) although this is uncommon. The likely absolute reduction in maternal deaths projected to Nepal was estimated to be low, while the implementation costs were high. Stakeholders also raised several concerns regarding feasibility, acceptability, appropriate dosing, and risk communication. Conclusions This review concludes that the blanket supplementation of calcium cannot be recommended in Nepal. A better approach may be to identify high-risk pregnant women and manage their antenatal visits and delivery to prevent mortality from pre-eclampsia

Is routine Vitamin A supplementation still justified for children in Nepal? Trial synthesis findings applied to Nepal national mortality estimates

Background The World Health Organization has recommended Vitamin A supplementation for children in low- and middle-income countries for many years to reduce child mortality. Nepal still practices routine Vitamin A supplementation. We examined the potential current impact of these programs using national data in Nepal combined with an update of the mortality effect estimate from a meta-analysis of randomized controlled trials. Methods We used the 2017 Cochrane review as a template for an updated meta-analysis. We conducted fresh searches, re-applied the inclusion criteria, re-extracted the data for mortality and constructed a summary of findings table using GRADE. We applied the best estimate of the effect obtained from the trials to the national statistics of the country to estimate the impact of supplementation on under-five mortality in Nepal. Results The effect estimates from well-concealed trials gave a 9% reduction in mortality (Risk Ratio: 0.91, 95% CI 0.85 to 0.97, 6 trials; 1,046,829 participants; low certainty evidence). The funnel plot suggested publication bias, and a meta-analysis of trials published since 2000 gave a smaller effect estimate (Risk Ratio: 0.96, 95% CI 0.89 to 1.03, 2 trials, 1,007,587 participants), with the DEVTA trial contributing 55.1 per cent to this estimate. Applying the estimate from well-concealed trials to Nepal’s under-five mortality rate, there may be a reduction in mortality, and this is small from 28 to 25 per 1000 live births; 3 fewer deaths (95% CI 1 to 4 fewer) for every 1000 children supplemented. Conclusions Vitamin A supplementation may only result in a quantitatively unimportant reduction in child mortality. Stopping blanket supplementation seems reasonable given these data

Paired SARS-CoV-2 spike protein mutations observed during ongoing SARS-CoV-2 viral transfer from humans to minks and back to humans

A mutation analysis of SARS-CoV-2 genomes collected around the world sorted by sequence, date, geographic location, and species has revealed a large number of variants from the initial reference sequence in Wuhan. This analysis also reveals that humans infected with SARS-CoV-2 have infected mink populations in the Netherlands, Denmark, United States, and Canada. In these animals, a small set of mutations in the spike protein receptor binding domain (RBD), often occurring in specific combinations, has transferred back into humans. The viral genomic mutations in minks observed in the Netherlands and Denmark show the potential for new mutations on the SARS-CoV-2 spike protein RBD to be introduced into humans by zoonotic transfer. Our data suggests that close attention to viral transfer from humans to farm animals and pets will be required to prevent build-up of a viral reservoir for potential future zoonotic transfer

Characterization of Genes Involved in Chromatic Acclimation in the Cyanobacterium Synechococcus sp. A 15-62

Synechococcus, a genus of photosynthetic cyanobacteria, is the second most abundant oxygenic microorganism in the marine environment that contributes significantly to the ocean’s primary productivity (Humily et al. 2013; Shukla et al. 2012). They are capable of utilizing available light of different wavelengths in the visible spectrum to perform photosynthesis and fix carbon dioxide and thus inhabit a wide range of light niches in the ocean along horizontal (coast vs offshore) and vertical gradients (depth) (Humily et al. 2013). A gene encoding a putative lyase isomerase, mpeQ, is present in phycoerythrin-II encoding operon that is expressed constitutively and a gene encoding putative lyase, mpeW, is present in CA-4 genomic island whose expression is regulated by ambient light color were identified and characterized in Synechococcus sp. A15- 62, a strain having a blue light specialist phenotype in its basal state. The amino acid sequence of the proteins encoded by mpeW and mpeQ are similar to other characterized lyases and these genes are conserved in cyanobacteria strains containing the CA4-B genomic island, which controls CA4 (Humily et al. 2013). The MpeW and MpeQ proteins were produced in E. coli and co-expressed with recombinant HT-MpeA and phycoerythrobilin (PEB) synthesis machinery. Site directed mutants of the HT-MpeA protein (Cys75Ala, Cys83Ala, Cys140Ala) were used to investigate the site for bilin attachment. The recombinant protein co-expression experiments of MpeQ and MpeW demonstrated that MpeQ attaches phycoerythrobilin (PEB) to cysteine-83 site on a-phycoerythrin II and isomerizes it to phycourobilin (PUB) and MpeW attaches phycoerythrobilin (PEB) to the same site

Diario de Córdoba de comercio, industria, administración, noticias y avisos: Año XXXVI Número 10637 - 27 Mayo 1885

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Adoption status of improved production technology in rice cultivation in Kanchanpur, Nepal

A study was carried out in 2020 to assess the scenario of the improved production technologies among rice growers in Kanchanpur and to identify the factors influencing the adoption of these technologies. The simple random sampling procedure was used to collect data from 90 respondents using a semi-structured interview schedule from Belauri, Bhimdutta municipality, and Beldandi rural municipality which are under the command area of the rice super zone, Kanchanpur. The information on prevailing cultural practice, production, and productivity, adoption of improved technology, problems/constraints faced by farmers in rice cultivation in the study area were collected from the farmers by interview. The data were processed, cleaned, and analyzed using software MS-excel and SPSS. The simple descriptive and inferential statistics like chi-square and binary logistic regression models were used to find the relationship between dependent and independent variables. Respondents adopted plant protection measures (chemical weed control, insects, and disease control) and seed treatment relatively less than they adopted recommended variety, Seed Replacement Rate (SRR), and storage treatment.  The majority of the respondents were affiliated with the farmers’ groups but the majority of them had not received training. Furthermore, spade, hoe, tractor, thresher, sickle, wooden plough bullock cart water pumps, tillers, reapers were used by respondents. Binary logistic regression revealed that membership of agriculture group, advice from agriculture technician, training, visit of extension workers and rice cultivated land had a positive and significant effect on the adoption of various production practices. Inadequate availability of fertilizers and inputs (0.85), Inadequate training (0.68), inadequate machinery availability (0.54) were the major constraints faced by the farmers on rice cultivation

Characterization of the Protein Encoded by unk9, a Conserved Gene of Unknown Function in Synechococcus sp. RS9916

Light harvesting antennae complexes of photosystem II, phycobilisomes, are anchored to the thylakoid membranes in cyanobacteria. These phycobilisomes are comprised of rods and a core containing phycobiliproteins (PBP) and linker polypeptides. The outer rods of the PBP have red protein-pigment complexes called phycoerythrin (PE), comprised of α- and β-subunits. Some species of Synechococcus contain two types of PE in the distal part of their rods, phycoerythrin class I (PE I) and phycoerythrin class II (PE II). Both PE I and PEII bind phycoerythrobilin (PEB) and PE II binds both phycoerythrobilin (PEB) and phycourobilin (PUB). These bilins are bound to PE due to the enzymatic activity of phycobilin lyases. The operon encoding various PE II-specific genes has a conserved gene of unknown function called unk9 (Six et al., 2007). The 332bp gene, unk9, lies adjacent to mpeY in the genome and encodes a protein which is uncharacterized, but is conserved in all PE-II containing organisms. A histidine-tagged (HT) form of unk9 was amplified by PCR and cloned in the pCOLA vector in order to produce recombinant HT-UNK9 protein in E. coli. The HT-UNK9 protein produced in E. coli was soluble. Because the unk9 gene is adjacent to mpeY, we hypothesized that his-tagged Unk9 may interact with other putative lyases such as MpeY or MpeU, affecting their activity. Recombinant protein co-expression experiments were completed to determine the function of Unk9. Results from this project will be presented. References: Six, Christophe, et al. Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study. Genome Biol 8.12 (2007): R259