101 research outputs found

    La construction de l'altérité dans l'espace noir Atlantique : Etats-Unis - France - Caraïbes - Amérique Latine

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    International audienceCe numéro de la Revue européenne des migrations internationales vise à restituer un dialogue interdisciplinaire dans lequel la discussion théorique s'alimente et s'enrichit de recherches empiriques en Amérique latine, en France, dans la Caraïbe et aux Etats-Unis, centrées sur des populations pouvant être catégorisées, selon les contextes, comme "noires", "africaines", "afrodescendantes", "afrocaribéennes", "antillaises", etc.La réflexion menée dans les articles publiés s'organise autour de deux enjeux principaux. D'une part dépasser la contradiction entre l'inexistence avérée des races humaines et l'existence constatée de groupes humains racialisés. D'autre part sonder la pertinence analytique et l'utilité opératoire d'une distinction entre ethnicisation et racialisation.Historiciser et (re)localiser les processus d'altérisation et de minorisation à l'oeuvre dans divers contextes de l'espace atlantique, ouvre la voie à une dénaturalisation de ces groupes. C'est pourquoi ces processus sont appréhendés comme des enjeux contestés et des ressources mobilisables par des groupes, inscrits dans des rapports de pouvoir

    Jardins

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    High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae)

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    International audienceHigh-density genetic maps were constructed for loci involved in nuclear male sterility (NMS1-locus) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L.). The mapping population consisted of 389 F1' individuals derived from a cross between two plants, K28 (male-sterile) and K59 (pollen-fertile), both heterozygous at the S-locus. This F1' mapping population segregated for both male sterility (MS) and strong self-incompatibility (SI) phenotypes. Phenotyping F1' individuals for MS allowed us to map the NMS1-locus to linkage group (LG) 5, while controlled diallel and factorial crosses to identify compatible/incompatible phenotypes mapped the S-locus to LG2. To increase the density of markers around these loci, bulked segregant analysis was used. Bulks and parental plants K28 and K59 were screened using amplified fragment length polymorphism (AFLP) analysis, with a complete set of 256 primer combinations of EcoRI-ANN and MseI-CNN. A total of 31,000 fragments were generated, of which 2,350 showed polymorphism between K59 and K28. Thirteen AFLP markers were identified close to the NMS1-locus and six in the vicinity of the S-locus. From these AFLP markers, eight were transformed into sequence-characterized amplified region (SCAR) markers and of these five showed co-dominant polymorphism. The chromosomal regions containing the NMS1-locus and the S-locus were each confined to a region of 0.8 cM. In addition, we mapped genes encoding proteins similar to S-receptor kinase, the female determinant of sporophytic SI in the Brasicaceae, and also markers in the vicinity of the putative S-locus of sunflower, but none of these genes or markers mapped close to the chicory S-locus

    E. coli Nissle 1917 is a safe mucosal delivery vector for a birch-grass pollen chimera to prevent allergic poly-sensitization

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    International audienceAllergic poly-sensitization affects a large number of allergic patients and poses a great challenge for their treatment. In this study we evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) expressing a birch and grass pollen allergen chimera ‘Bet v 1, Phl p 1 and Phl p 5’ (EcN-Chim) on allergy prevention after oral or intranasal application in poly-sensitized mice. In contrast to oral application, intranasal pretreatment with EcN-Chim prior to poly-sensitization led to a significant reduction of lung inflammation (eosinophils, IL-5, and IL-13 in bronchoalveolar lavage) along with suppressed levels of allergen-specific serum IgE. The suppression was associated with increased levels of allergen-specific IgA in lungs and serum IgG2a along with increased Foxp3, TGF-β, and IL-10 mRNA in bronchial lymph nodes. In vitro EcN induced high levels of IL-10 and IL-6 in both lung and intestinal epithelial cells. Importantly, using in vivo imaging techniques we demonstrated that intranasally applied EcN do not permanently colonize nose, lung, and gut and this strain might therefore be a safe delivery vector against allergy in humans. In conclusion, our data show that intranasal application of recombinant EcN expressing a multiallergen chimera presents a novel and promising treatment strategy for prevention of allergic poly-sensitization
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