Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins

[EN] Information concerning the virulence factor genes and antibiotic resistance of rabbit enterococci is limited, so in this study we tested the virulence factor genes in Enterococcus faecalis strains from rabbits. Moreover, their resistance/sensitivity to antibiotics and sensitivity to enterocins was also tested, with the aim of contributing to our enterocin spectra study and to indicate the possibility of enterocin application in prevention or contaminant elimination in rabbit husbandry. A total of 144 rabbit samples were treated using a standard microbiological method. Thirty-one pure colonies of the species Enterococcus faecalis were identified, using the MALDI-TOF identification system and confirmed using phenotyping, among which 15 strains were virulence factor gene absent. The gelE gene was the most detected (42%); however, the expression of gelatinase phenotype did not always correlate with the detection of gelE. Strains did not show ß-haemolysis and were mostly resistant to tested antibiotics, but sensitive to enterocins (Ent), mainly to Ents EK13=A (P), 2019 and Ent M. Rabbit E. faecalis strains displayed antibiotic resistant traits and the presence of expressed and silent virulence genes, but they showed high levels of sensitivity to natural antimicrobials-enterocins, which indicates the possible prevention of multidrug and virulent enterococcal contaminants by enterocins.This study was supported by Project 2/0004/14 and 2/0006/17. The care and experimental procedures with animals followed the guidelines stated in the Guide for the Care and Use of Laboratory Animals (1996) and the trials were accepted by the Ethical Commission of the Institute of Animal Physiology in Košice and by the Slovak Veterinary and Food Administration. We are grateful to Ľubica Chrastinová from the National Agricultural and Food Centre, Nitra for her skillful assistance and sampling.Pogány Simonová, M.; Lauková, A. (2017). Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins. World Rabbit Science. 25(1):63-71. https://doi.org/10.4995/wrs.2017.5694SWORD637125

Bacteriocin-producing strain lactiplantibacillus plantarum LP17L/1 isolated from traditional stored Ewe’s milk cheese and its beneficial potential

Research Areas: Food Science & TechnologyStored ewe’s milk lump cheese is a local product that can be a source of autochthonous beneficial microbiota, especially lactic acid bacteria. The aim of this study was to show the antimicrobial potential of Lactiplantibacillus plantarum LP17L/1 isolated from stored ewe’s milk lump cheese. Lpb. plantarum LP17L/1 is a non-hemolytic, non-biofilm-forming strain, susceptible to antibiotics. It contains genes for 10 bacteriocins—plantaricins and exerted active bacteriocin with in vitro antistaphylococcal and anti-listerial effect. It does not produce damaging enzymes, but it produces β-galactosidase. It also sufficiently survives in Balb/c mice without side effects which indicate its safety. Moreover, a reduction in coliforms in mice jejunum was noted. LP17L/1 is supposed to be a promising additive for Slovak local dairy products.info:eu-repo/semantics/publishedVersio