Bioassay of the Nucleopolyhedrosis Virus of \u3ci\u3eNeodiprion Sertifer\u3c/i\u3e (Hymenoptera: Diprionidae)

Linear regression analysis of probit mortality versus several concentrations of nucleo- polyhedrosis virus of Neodiprion sertifer resulted in the equation Y = 2.170 + 0.872X. An LC50 was calculated at 1758 PIB/mL Also, the incubation time of the virus was dependent on Its concentration

Field Release of Virus-Sprayed Adult Parasitoids of the European Pine Sawfly (Hymenoptera: Diprionidae) in Wisconsin

Rapid field release of adult parasitoids sprayed with the nucleopolyhedrosis virus of the European pine sawfly successfully transferred the virus to feeding larval colonies

Temperature and Crowding Effects on Virus Manifestation in Neodiprion Sertifer (Hymenoptera: Diprionidae) Larvae

Temperature and (or) crowding (larval density) functioned as stressors in the induction of symptoms associated with the nucleopolyhedrosis virus of the European pine sawfly, Neodiprion sertifer, Subsamptes of larvae maintained at 30 and 35°C, with three levels of larval density each (20, 60, and 100/shoot) which had died under these conditions, revealed the presence of polyhedral inclusion bodies under microscopic examination. In contrast, larvae maintained at 25°C with the same three larval density levels experienced no symptoms of virus infection or mortality, The latter was consistent with field observations when temperatures during larval development ranged from 14°C to 27°C and larval densities were in the same general range