Multi-Omic Profiling of Melophlus Sponges Reveals Diverse Metabolomic and Microbiome Architectures that Are Non-overlapping with Ecological Neighbors.

Marine sponge holobionts, defined as filter-feeding sponge hosts together with their associated microbiomes, are prolific sources of natural products. The inventory of natural products that have been isolated from marine sponges is extensive. Here, using untargeted mass spectrometry, we demonstrate that sponges harbor a far greater diversity of low-abundance natural products that have evaded discovery. While these low-abundance natural products may not be feasible to isolate, insights into their chemical structures can be gleaned by careful curation of mass fragmentation spectra. Sponges are also some of the most complex, multi-organismal holobiont communities in the oceans. We overlay sponge metabolomes with their microbiome structures and detailed metagenomic characterization to discover candidate gene clusters that encode production of sponge-derived natural products. The multi-omic profiling strategy for sponges that we describe here enables quantitative comparison of sponge metabolomes and microbiomes to address, among other questions, the ecological relevance of sponge natural products and for the phylochemical assignment of previously undescribed sponge identities

Cognitive-Behavioral Treatment for Child and Adolescent Anxiety: The \u3cem\u3eCoping Cat Program\u3c/em\u3e

Anxiety disorders are common psychological disorders experienced by youth (Warren & messer, 1999), with reported rates of 10-20% in the general population and primary care settings (Chavira, Stein, Bailey, & Stein, 2004; Costello, Mustillo, Keeler, & Angold, 2004). Anxiety disorders in youth include generalized anxiety disorder (GAD), social phobia (SP), separation anxiety disorder (SAD), specific phobias, obsessive-compulsive disorder (OCD), and post-traumatic stress disorder (PTSD) (APA, 1994). In this chapter, we focus on treatment for the three youth anxiety disorders: GAD, SP, and SAD

Removing Blinders from the Judiciary: In Re Artt Brennan, Kirby as an Evolutionary Step in the United States-United Kingdom Extradition Scheme

Within the extradition treaty between the United States and the United Kingdom, there exists an exemption for fugitives who have allegedly committed crimes that are political in nature. The political offense exemption has resulted in complications when dealing with the thorny issue of alleged terrorists from Northern Ireland who flee to the United States. This Note traces the history of the political offense exemption and scrutinizes the results of a recent Ninth Circuit decision, In re Artt, Brennan, Kirby, in which the court concluded that federal courts may inquire into systemic bias when considering whether or not a defendant\u27s conviction in the Northern Ireland judicial system was tainted by prejudice

Cognitive-Behavioral Therapy with Youth

Cognitive-behavioral therapies (CBTs) with children and adolescents use enactive, performance-based procedures, as well as cognitive interventions to produce changes in thinking, feeling, and behavior. Various forms of CBT have a common goal to help the child develop a constructive worldview and a problem-solving attitude. The problem-solving orientation can also be referred to as a coping template. Through the provision of carefully planned experiences, CBT helps the child and family build an adaptive, problem-solving perspective

FUS is sequestered in nuclear aggregates in ALS patient fibroblasts

Mutations in the RNA-binding protein FUS have been shown to cause the neurodegenerative disease amyotrophic lateral sclerosis (ALS). We investigate whether mutant FUS protein in ALS patient–derived fibroblasts affects normal FUS functions in the nucleus. We investigated fibroblasts from two ALS patients possessing different FUS mutations and a normal control. Fibroblasts from these patients have their nuclear FUS protein trapped in SDS-resistant aggregates. Genome-wide analysis reveals an inappropriate accumulation of Ser-2 phosphorylation on RNA polymerase II (RNA Pol II) near the transcription start sites of 625 genes for ALS patient cells and after small interfering RNA (siRNA) knockdown of FUS in normal fibroblasts. Furthermore, both the presence of mutant FUS protein and siRNA knockdown of wild-type FUS correlate with altered distribution of RNA Pol II within fibroblast nuclei. A loss of FUS function in orchestrating Ser-2 phosphorylation of the CTD of RNA Pol II is detectable in ALS patient–derived fibroblasts expressing mutant FUS protein, even when the FUS protein remains largely nuclear. A likely explanation for this loss of function is the aggregation of FUS protein in nuclei. Thus our results suggest a specific mechanism by which mutant FUS can have biological consequences other than by the formation of cytoplasmic aggregates

International Veterinary Epilepsy Task Force consensus proposal: Medical treatment of canine epilepsy in Europe

In Europe, the number of antiepileptic drugs (AEDs) licensed for dogs has grown considerably over the last years. Nevertheless, the same questions remain, which include, 1) when to start treatment, 2) which drug is best used initially, 3) which adjunctive AED can be advised if treatment with the initial drug is unsatisfactory, and 4) when treatment changes should be considered. In this consensus proposal, an overview is given on the aim of AED treatment, when to start long-term treatment in canine epilepsy and which veterinary AEDs are currently in use for dogs. The consensus proposal for drug treatment protocols, 1) is based on current published evidence-based literature, 2) considers the current legal framework of the cascade regulation for the prescription of veterinary drugs in Europe, and 3) reflects the authors’ experience. With this paper it is aimed to provide a consensus for the management of canine idiopathic epilepsy. Furthermore, for the management of structural epilepsy AEDs are inevitable in addition to treating the underlying cause, if possible