Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay

The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg2+ as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide

Liquefaction of sunflower husks for biochar production

MSc (Engineering Sciences in Chemical Engineering), North-West University, Potchefstroom Campus, 2014Biochar, a carbon-rich and a potential solid biofuel, is produced during the liquefaction of biomass. Biochar can be combusted for heat and power, gasified, activated for adsorption applications, or applied to soils as a soil amendment and carbon sequestration agent. It is very important and advantageous to produce biochar under controlled conditions so that most of the carbon is converted. The main objective of the study was to investigate the effect of solvents, reaction temperature and reaction atmosphere on biochar production during the liquefaction of sunflower husks. The liquefaction of sunflower husks was initially investigated in the presence of different solvents (water, methanol, ethanol, iso-propanol and n-butanol) to study the effect of solvents on biochar yields. The experiments were carried out in an SS316 stainless steel high pressure autoclave at 280°C, 30 wt.% biomass loading in a solvent and starting pressure of 10 bar. Secondly, sunflower husks were liquefied at various temperatures (240-320°C) to assess the influence of reaction temperature on the biochar yield. Experiments were carried out under either a carbon dioxide or nitrogen atmosphere with a residence time of 30 minutes. Biochar samples obtained from sunflower husk liquefaction were structurally characterised by scanning electron microscopy (SEM) and Brunauer-Emmet-Teller (BET) analysis to compare surface morphological changes and pore structural changes at different reaction temperatures. Compositional analysis was done on sunflower husk biochar samples by proximate analysis, Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and Elemental analysis. The results showed that biochar produced through the liquefaction of sunflower husks was significantly affected by the type of solvent used. The highest biochar yields were obtained when ethanol was used (57.35 wt. %) and the lowest yields were obtained when n-butanol was used as a solvent (41.5 wt. %). A temperature of 240°C was found to produce the highest biochar yield (64 wt. %). However, biochar yields decreased with increasing liquefaction temperature and the lowest yield was 41wt. % at 320°C. Temperature had the most significant influence on biochar yield in an N₂ atmosphere, while solvent choice had the most significant influence on biochar yield in a CO₂ atmosphere. Temperature also had an effect on the structure of biomass, as the SEM analysis shows the biochar became more porous with increasing temperature. Generally, results from the CO₂ adsorption analysis, suggested that CO₂ develops microporosity to a greater extent than N₂ reaction. The results of sunflower husk compositional analysis show that sunflower husks contain a high lignin content (34.17 wt. %), of which the high lignin content in biomass is associated with high heating value and high solid yield product. Sunflower husks as waste product can be used to produce useful products such as biochar through liquefaction, and biochar can be used to generate heat and as a soil amendment due to its high heating value and high porosity. While these preliminary studies appear promising for the conversion of sunflower husks to biochar, further studies are needed.Master