Stocking Rate and Supplementation Effects on Performance of Lambs Grazing Triticale and Ryegrass Sward in Uruguay

An experiment was carried out from 9 June to 2 October 1997, using a Lolium multiflorum Lom. (Ryegrass) and X Triticosecale Wittmack (triticale) sward to evaluate the effect of stocking rate (SR; 20, 30 and 40 lambs/ha), and supplementation (S; with or without) on Corriedale lamb performance. SR had a significant effect on: liveweight gain (160, 130 and 90 g/an/d, P\u3c 0.01); greasy fleece weight (2.8, 2.7 and 2.3 kg, P\u3c 0.01); fiber diameter (28, 27, and 26 microns, P\u3c 0.05); rib eye depth (2.4, 2.1 and 2.0 cm, P\u3c 0.05); fat cover (3.2, 2.1 and 1.5 cm, P\u3c 0.01); hot carcass weight (17.7, 15.6 and 13.5 kg/an, P\u3c 0.01); GR (10.5, 6.6 and 4.2 mm, P\u3c 0.01) and boneless leg weight (1.56, 1.42 and 1.24 kg, P\u3c 0.05), for 20, 30 and 40 lambs per ha, respectively. At the highest SR, lambs increased grazing time (59 vs 52%) and biting rate (29 vs 26 bites/lamb/min). The mayor influence of S on lamb performance was found in grazing behavior variables: (grazing time (63 vs 50%, P\u3c 0.01); biting rate (26.8 vs 28.4 bites/lamb/min, P\u3c 0.01)), carcass characteristics: (hot carcass weight (15.3 vs 16.0 kg, P\u3c 0.05); GR (7.9 and 6.3 mm, P\u3c 0.05)) for with and without supplement, respectively. Over the experimental period (115 days), liveweight production and wool production ranged from 358 to 437 kg/ha for 20 and 30 lambs/ha respectively, and wool production from 55 to 93 kg/ha for 20 and 40 lambs/ha respectively. These results show the potential use of mixed ryegrass and triticale swards to produce high quality lamb meat even at high SRs, and the convenience of using supplements only when sward conditions are not sufficient to maintain an adequate lamb performance, particularly when high lamb SRs are used

Lamb Stocking Rate and Supplementation Effects on Mixed Triticale and Ryegrass Swards Characteristics

An experiment was carried out from 9 June to 22 October 1997, using a Lolium multiflorum L. (ryegrass) and Triticosecale Wittmack (triticale) mixed sward grazed by lambs in a rotational grazing system, to determine the effect of stocking rate (SR; 20, 30 and 40 lambs/ha) and supplementation (S; with or without) on herbage production, composition and nutritive value. SR affected significantly before and after grazing herbage mass and sward height, being higher the values of these variables at the lower SR (3232, 2611 and 2345 kg DM ha-1, P \u3c 0.05; 2557, 1761 and 1612 kg DM ha-1, P \u3c 0.01; 17, 11 and 9 cm, P \u3c 0.01 respectively). Increments in SR had an effect on post grazing sward composition, increasing the proportion of ryegrass (32, 36 and 47 %, P\u3c 0.05) and decreasing triticale contribution (68, 64 and 55 %, P\u3c 0.05) for 20, 30 and 40 lambs/ha. The effect of SR on sward nutritive value was not very clear. Before grazing, S affected significantly herbage mass, being higher the values at the supplemented treatments (2787 vs. 2672 kg DM ha-1, P \u3c 0.10). This experiment showed the high potential of forage production and nutritive value of ryegrass and triticale swards for lamb production in the sandy soil region of Uruguay, and the dominant effect of SR, compared to S, on most of the sward variables considered

The 3Rs: reduction and refinement through a multivariate statistical analysis approach in a behavioural study to unveil anxiolytic effects of natural extracts of Tilia Tomentosa.

Proceedings of the second Centro 3R Annual Meeting - Young Scientists | June 20-21, 2019, Genoa (Italy

Use of an animal model to evaluate anxiolytic effects of dietary supplementation with tilia tomentosa moench bud extracts

Anxiety disorders are common and complex psychiatric syndromes affecting a broad spectrum of patients. On top of that, we know that aging produces an increase in anxiety vulnerability and sedative consumption. Moreover, stress disorders frequently show a clear gender susceptibility. Currently, the approved pharmacological strategies have severe side effects such as hallucinations, addiction, suicide, insomnia, and loss of motor coordination. Dietary integration with supplements represents an intriguing strategy for improving the efficacy and the safety of synthetic anxiolytics. Accordingly, a recent article demonstrated that glyceric bud extracts from Tilia tomentosa Moench (TTBEs) exert effects that are consistent with anxiolytic activity. However, the effects of these compounds in vivo are unknown. To examine this question, we conducted behavioral analysis in mice. A total of 21 days of oral supplements (vehicle and TTBEs) were assessed by Light Dark and Hole Board tests in male and female mice (young, 3 months; old, 24 months). Interestingly, the principal component analysis revealed gender and age-specific behavioral modulations. Moreover, the diet integration with the botanicals did not modify the body weight gain and the daily intake of water. Our results support the use of TTBEs as dietary supplements for anxiolytic purposes and unveil age and gender-dependent responses

Romidepsin in peripheral and cutaneous T-cell lymphoma: mechanistic implications from clinical and correlative data

Romidepsin is an epigenetic agent approved for the treatment of patients with cutaneous or peripheral T-cell lymphoma (CTCL and PTCL). Here we report data in all patients treated on the National Cancer Institute 1312 trial, demonstrating long-term disease control and the ability to retreat patients relapsing off-therapy. In all, 84 patients with CTCL and 47 with PTCL were enrolled. Responses occurred early, were clinically meaningful and of very long duration in some cases. Notably, patients with PTCL receiving romidepsin as third-line therapy or later had a comparable response rate (32%) of similar duration as the total population (38%). Eight patients had treatment breaks of 35months to 10years; in four of six patients, re-initiation of treatment led to clear benefit. Safety data show slightly greater haematological and constitutional toxicity in PTCL. cDNA microarray studies show unique individual gene expression profiles, minimal overlap between patients, and both induction and repression of gene expression that reversed within 24h. These data argue against cell death occurring as a result of an epigenetics-mediated gene induction programme. Together this work supports the safety and activity of romidepsin in T-cell lymphoma, but suggests a complex mechanism of action

Efficacy of a vegetal mixture composed of Zingiber officinale, Echinacea purpurea, and Centella asiatica in a mouse model of neuroinflammation: In vivo and ex vivo analysis

Experimental evidence suggests that neuroinflammation is a key pathological event of many diseases affecting the nervous system. It has been well recognized that these devastating illnesses (e.g., Alzheimer's, Parkinson's, depression, and chronic pain) are multifactorial, involving many pathogenic mechanisms, reason why pharmacological treatments are unsatisfactory. The purpose of this study was to evaluate the efficacy of a vegetal mixture capable of offering a multiple approach required to manage the multifactoriality of neuroinflammation. A mixture composed of Zingiber officinale (150 mg kg(-1)), Echinacea purpurea (20 mg kg(-1)), and Centella asiatica (200 mg kg(-1)) was tested in a mouse model of systemic neuroinflammation induced by lipopolysaccharide (LPS, 1 mg kg(-1)). Repeated treatment with the vegetal mixture was able to completely counteract thermal and mechanical allodynia as reported by the Cold plate and von Frey tests, respectively, and to reduce the motor impairments as demonstrated by the Rota rod test. Moreover, the mixture was capable of neutralizing the memory loss in the Passive avoidance test and reducing depressive-like behavior in the Porsolt test, while no efficacy was shown in decreasing anhedonia as demonstrated by the Sucrose preference test. Finally, LPS stimulation caused a significant increase in the activation of glial cells, of the central complement proteins and of inflammatory cytokines in selected regions of the central nervous system (CNS), which were rebalanced in animals treated with the vegetal mixture. In conclusion, the vegetal mixture tested thwarted the plethora of symptoms evoked by LPS, thus being a potential candidate for future investigations in the context of neuroinflammation