High resolution imaging of neoplasma using optical coherence tomography

Thesis (Ph. D.)--Harvard--Massachusetts Institute of Technology Division of Health Sciences and Technology, 2000.Includes bibliographical references (leaves 145-156).Diagnostic imaging technologies for the detection of cancer include CT, MRI, ultrasonography, and endoscopy. However, many early neoplastic changes remain beyond their detection limits. A modality capable of imaging at or near the cellular level could detect disease at earlier stages than currently possible and thus improve patient prognosis. Optical coherence tomography (OCT) can achieve resolutions in the cellular and subcellular range (1-15 [mu]m) and could improve the diagnostic range of clinical imaging procedures. The research described in this thesis includes the ex vivo imaging surveys, technology development and clinical studies necessary to demonstrate the feasibility of OCT imaging for the in situ diagnosis of premalignant and neoplastic lesions. The first step in this endeavor was to evaluate the performance of OCT using ex vivo tissue specimens and identify the features in the OCT images which can be used for the diagnosis of disease. The development of the technologies necessary to introduce OCT to clinical settings, including a portable system and imaging devices, ensued. Enhancements in the post processing and visualization algorithms were introduced and the feasibility of ultra-high resolution and spectroscopic imaging was evaluated. In vivo OCT imaging, to assess the performance of the technology in clinical scenarios, followed in two systems, the cervical and oral mucosa. OCT can function as a type of optical biopsy to yield image information with resolutions approaching that of conventional histopathology in situ, without the need for excision and processing. OCT can be integrated to a wide range of clinical instruments including endoscopes, catheters, laparascopes, and surgical probes. A role for OCT is envisioned in clinical scenarios such as the follow-up management of patients with cervical intraepithelial neoplasia, on tamoxifen, with gastro-esophageal reflux or Barrett's and also guidance of surgical procedures in sensitive areas. Further improvements of the OCT technology will most likely enhance the abilities of the technique and may eventually lead to its establishment as a a powerful imaging modality for the diagnosis and management of cancer.by Constantinos Pitris.Ph.D

Optics and Quantum Electronics

Contains table of contents for Section 3, reports on eighteen research projects and a list of publications.Defense Advanced Research Projects Agency Grant F49620-96-0126U.S. Air Force - Office of Scientific Research Grant F49620-98-1-0139Defense Advanced Research Projects AgencyJoint Services Electronics ProgramU.S. Air Force - Office of Scientific ResearchNational Science FoundationU.S. Navy - Office of Naval ResearchCharles S. Draper LaboratoryNational Science Foundation/MRSECJoint Services Electronics Program Grant DAAH04-95-1-0038U.S. Air Force - Office of Scientific Research Contract F49620-95-1-0221U.S. Navy - Office of Naval Research (MFEL) Contract N00014-91-J-1956U.S. Navy - Office of Naval Research (MFEL) Contract N000014-97-1-1066National Institutes of Health Grant 9RO1 EY11289-12National Institutes of Health Grant 1R01 CA75289-01U.S. Navy - Office of Naval Research/MFEL Contract N00014-94-1-071