Bacterial community patterns of domestic water sources in the Gogogo and Nkonkobe areas of the Eastern Cape Province, South Africa

In the present study we have characterised the microbiological quality of the drinking water from different water sources in Gogogo and Nkonkobe areas in the Eastern Cape Province, South Africa. Using both standard microbiological methods and the commercial API 20E assay kit, we have been able to identify 54 different species of micro-organisms, 77.5% of which proved to be human pathogens, 53.2% of them, belonging to the family Enterobacteriacae and 22.5% were non-pathogens. Further investigations revealed a marked difference both in terms of the number and in the variety of organisms during different seasons. Thus the highest number of bacteria, i.e. 45.74% for Gogogo drinking water sources and 48.1% for Nkonkobe was registered in winter, while the lowest, i.e. 14.18%, for Gogogo and 10.94% for Nkonkobe water sources was recorded in spring. Considering the overall bacterial community pattern of domestic water sources in the Gogogo and Nkonkobe areas, it can be concluded that the water is of poor microbial quality and requires extensive purification before any domestic use can be considered. WaterSA Vol.30 (3) 2004: 341-34

Microbiological and physico-chemical assessment of the quality of domestic water sources in selected rural communities of the Eastern Cape Province, South Africa

The domestic raw water sources in Nkonkobe and Gogogo were characterised by using both microbiological and standard physical methods to investigate the quality of the water at the sampling sites. For microbiological analysis, indicator bacteria namely, heterotrophic bacteria, total and faecal coliforms and for physical parameters, pH, turbidity and temperature were assessed to check whether the distributed water as well as the water from dams, and rivers was safe for drinking and other domestic uses. The water quality parameters of concern were microbial contamination and turbidity. Almost all the indicator bacteria counts and turbidity values were above the South African recommended limits. Both Nkonkobe and Gogogo raw water sources had a poor water quality. The water was unfit for human consumption without prior treatment. The quality of the water source depended on local conditions. This indicated that poor sanitation and hygiene conditions and lack of, or little environmental awareness among the people in rural areas, could be considered as the major causes of source water contamination. WaterSA Vol.30 (3) 2004: 333-34

Genetic diversity and in vitro antibiotic susceptibility profile of Salmonella species isolated from domestic water and wastewater sources in the Eastern Cape Province of South Africa

We assessed the genetic diversity of forty Salmonella isolates obtained from selected domestic water and waste water sources in the Eastern Cape Province of South Africa using DNA fingerprinting and antibiotic susceptibility profile as test indices. Restriction digests and SDS/PAGE as well as the DNA dendograms of the isolates revealed that most of these strains show a high percentage of genetic similarity, and suggests that these methods are valuable tools for evaluating the relatedness ofSalmonella species. Also, of the seven antibiotics and sulfonamides tested against the Salmonella species, five namely, neomycin, chloramphenicol, kanamycin, streptomycin and cotriomoxazole were significantly inhibitory, while the bacteria showed considerable resistance to doxycycline andsulfomethoxazole