Defect extensions and a characterization of tame fields

We study the relation between two important classes of valued fields: tame fields and defectless fields. We show that in the case of valued fields of equal characteristic or rank one valued fields of mixed characteristic, tame fields are exactly the valued fields for which all algebraic extensions are defectless fields. In general tame fields form a proper subclass of valued fields for which all algebraic extensions are defectless fields. We introduce a wider class of roughly tame fields and show that every algebraic extension of a given valued field is defectless if and only if its henselization is roughly tame. Proving the above results we also present constructions of Galois defect extensions in positive as well as mixed characteristic.Comment: 18 page

An antagonist of ATP-regulated potassium channels, the guanidine derivative U-37883A, stimulates the synthesis of phosphatidylserine in rat liver endoplasmic reticulum membranes

AbstractThe guanidine derivative U-37883A has been found to stimulate in vitro synthesis of phosphatidylserine in endoplasmic reticulum membranes, catalyzed exclusively by a serine-specific base exchange enzyme. The stimulation of the enzyme activity by the drug was concentration-dependent, with EC50 of 54 μM, while the biologically inactive analog of U-37883A, U-42069, was without effect. The stimulation caused by U-37883A was enhanced under the conditions when active transport of Ca2+ into the lumen of microsomal vesicles was induced, whereas it was inhibited by a calcium ionophore, A23187, and by a specific inhibitor of Ca2+-ATPase, thapsigargin. On the other hand, a potassium ionophore, valinomycin, had no effect on phosphatidylserine synthesis. U-37883A did not affect the Km of the base exchange enzyme for serine, but greatly reduced the EC50 value of the enzyme for calcium. Furthermore, Ca2+ uptake by endoplasmic reticulum vesicles has been found to increase in the presence of U-37883A. These observations suggest that U-37883A enhances phosphatidylserine synthesis indirectly by acting on calcium transport, thus affecting calcium concentration within the lumen of endoplasmic reticulum membranes. Alternatively, the effect of the drug could be propagated via the mechanism by which phospholipid flip-flop movement, known to regulate the serine-specific base exchange reaction, is modulated

LoRaWAN communication implementation platforms

A key role in the development of smart Internet of Things (IoT) solutions is played by wireless communication technologies, especially LPWAN (Low-Power Wide-Area Network), which are becoming increasingly popular due to their advantages: long range, low power consumption and the ability to connect multiple edge devices. However, in addition to the advantages of communication and low power consumption, the security of transmitted data is also important. End devices very often have a small amount of memory, which makes it impossible to implement advanced cryptographic algorithms on them. The article analyzes the advantages and disadvantages of solutions based on LPWAN communication and reviews platforms for IoT device communication in the LoRaWAN (LoRa Wide Area Network) standard in terms of configuration complexity. It describes how to configure an experimental LPWAN system being built at the Department of Computer Science and Telecommunications at Poznan University of Technology for research related to smart buildings

LoRaWAN communication implementation platforms

A key role in the development of smart Internet of Things (IoT) solutions is played by wireless communication technologies, especially LPWAN (Low-Power Wide-Area Network), which are becoming increasingly popular due to their advantages: long range, low power consumption and the ability to connect multiple edge devices. However, in addition to the advantages of communication and low power consumption, the security of transmitted data is also important. End devices very often have a small amount of memory, which makes it impossible to implement advanced cryptographic algorithms on them. The article analyzes the advantages and disadvantages of solutions based on LPWAN communication and reviews platforms for IoT device communication in the LoRaWAN (LoRa Wide Area Network) standard in terms of configuration complexity. It describes how to configure an experimental LPWAN system being built at the Department of Computer Science and Telecommunications at Poznan University of Technology for research related to smart buildings

Human SUV3 helicase regulates growth rate of the HeLa cells and can localize in the nucleoli

The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle

The role of alpha-1-antitrypsin protein in the pathogenesis of abdominal aortic aneurysm

Alpha-1-antitrypsin is a potent antiprotease playing an important role in maintaining protease-antiproteasebalance. It protects the structures of extracellular matrix against destruction by proteolytic enzymes. Loss ofelasticity occurs when increased protease activity is accompanied by qualitative impairment or reduced concentrationsof antiproteases. Alpha-1-antitrypsin deficiency is a risk factor for obstructive lung disease, includingemphysema, liver and kidney disorders and, less often, follicular panniculitis, granulomatosis with polyangiitis(previously Wegener’s granulomatosis). Literature also emphasises the role of AAT in the development of aorticaneurysms, and results of biochemical studies support this theory. Aortic aneurysm is an important clinicalproblem, unceasingly associated with high mortality. For this reason, it is exceptionally important to identify itsrisk factors. Studies on the relationship between AAT and development of AAA (abdominal aortic aneurysm)have been conducted since the 1990s. Due to the development in molecular diagnostic techniques, new reportson the topic appeared over the last decade

Selenium and zinc biofortification of Pleurotus eryngii mycelium and fruiting bodies as a tool for controlling their biological activity

Pleurotus eryngii (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of P. eryngii is characterized by the ability of effective bio-elements absorption from growth media so it could be biofortified with trace elements with a functional activity in the human body. In this study, the ability of P. eryngii mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their effectiveness in zinc and selenium accumulation. The effect of Se and Zn biofortification on productivity, chemical compounds, and bio-elements content of P. eryngii was determined as well. To enhance Se and Zn content in P. eryngii fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg $L^{-1}$, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg $L^{-1}$, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20mg $L^{-1}$. The content of organic compounds include phenolic compounds and lovastatin, which were determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of P. eryngii to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be di erent depending on the type of salt used. The present study also showed that conducting mycelium of P. eryngii in in vitro culture, with a higher content of zinc ions, can result in obtaining the materials with better antioxidant ability. The results of this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements

Enhancing waste resource efficiency: circular economy for sustainability and energy conversion

This study explores optimizing waste resource efficiency through the Circular Economy (CE) framework. Motivated by the imperative to enhance resource efficiency and mitigate waste’s environmental impact, we examine the CE concept’s extension of product life cycles, while minimizing waste. We conduct a comprehensive review to explore the core CE principles across all stages of the product lifecycle and provide an in-depth analysis of waste treatment practices in Poland, emphasizing potential energy conversion methods like biogas production and incineration. Our findings underscore the importance of prioritizing waste incineration plant design for quicker payback, aligning with circular rational economic practices. Strategies such as improving biogas production, establishing solid bio-waste fermentation facilities, promoting sortable packaging, and incentivizing sustainable sorting emerge to optimize waste management. These findings highlight the pivotal role of economic, environmental, and energy considerations in shaping sustainable waste management strategies