Using metabarcoding to assess Viridiplantae sequence diversity present in Antarctic glacial ice

Antarctica contains most of the glacial ice on the planet, a habitat that is largely unexplored by biologists. Recent warming in parts of Antarctica, particularly the Antarctic Peninsula region, is leading to widespread glacial retreat, releasing melt water and, potentially, contained biological material and propagules. In this study, we used a DNA metabarcoding approach to characterize Viridiplantae DNA present in Antarctic glacial ice. Ice samples from six glaciers in the South Shetland Islands and Antarctic Peninsula were analysed, detecting the presence of DNA representing a total of 16 taxa including 11 Chlorophyta (green algae) and five Magnoliophyta (flowering plants). The green algae may indicate the presence of a viable algal community in the ice or simply of preserved DNA, and the sequence diversity assigned included representatives of Chlorophyta not previously recorded in Antarctica. The presence of flowering plant DNA is most likely to be associated with pollen or tissue fragments introduced by humans

Fairy ring disease affects epiphytic algal assemblages associated with the moss Sanionia uncinata (Hedw.) Loeske (Bryophyta) on King George Island, Antarctica

Since the nineteenth century, a ring-forming disease attacking Antarctic mosses has been reported. However, to date, only the effects on the mosses themselves are known. In this study, we used DNA metabarcoding to investigate the effects on the moss epiphytic algal community at different stages of disease progression. As the disease progressed, algal species richness decreased, although overall abundance was not significantly affected. Prasiolales appeared unaffected, whereas Ulotrichales were more sensitive. Trebouxiales dominated the advanced disease stage, suggesting a possible benefit from the disease, either through the elimination of competition or creation of new niches. Infection is responsible for moss death, leading to habitat loss for other organisms, but pathogenic effects on algae cannot be ruled out. Our data indicate that the disease not only impacts mosses but also other groups, potentially resulting in loss of Antarctic biodiversity. This study provides the first report of the disease effects on epiphytic algal communities of Antarctic bryophytes

The largest moss carpet transplant in Antarctica and its bryosphere cryptic biodiversity

As part of the reconstruction of the Brazilian Antarctic Station on King George Island, three areas of moss carpet were transplanted to minimize the impact of the new facilities on the local biodiversity. A total of 650 m2 of moss carpet was transplanted to neighboring but previously uncolonized locations and has subsequently survived for the last 3 years. Antarctic moss carpets typically comprise low moss species diversity and are often monospecific. We investigated the cryptic biodiversity that was transplanted along with the carpets using a metabarcoding approach through high throughput sequencing. We targeted 16S rRNA for Bacteria and Archaea, ITS for Fungi and Viridiplantae and Cox1 for Metazoa. We detected DNA representing 263 taxa from five Kingdoms (Chromista, Fungi, Metazoa, Protista and Viridiplantae), two Domains (Archaea and Bacteria) and 33 Phyla associated with the carpet. This diversity included one Archaea, 189 Bacteria, 24 Chromista, 19 Fungi, eight Metazoa, seven Protista and 16 Viridiplantae. Bacteria was the most abundant, rich and diverse group, with Chromista second in diversity and richness. Metazoa was less diverse but second highest in dominance. This is the first study to attempt transplanting a significant area of moss carpet to minimize anthropogenic environmental damage in Antarctica and to use metabarcoding as a proxy to assess diversity associated with Antarctic moss carpets, further highlighting the importance of such habitats for other organisms and their importance for conservation