Pulmonary mycobiome of patients with suspicion of respiratory fungal infection – an exploratory study

Objective: This pilot study aimed to characterize the pulmonary mycobiome of patients with suspicion of fungal infection of the respiratory tract as well as to identify potentially pathogenic fungi colonizing/infecting their lungs. Methods: A cohort of 10 patients was analyzed, including HIV+ patients and patients with active infection caused by Mycobacterium species. Their respiratory samples (bronchoalveolar lavage fluid/ bronchial secretions) were pre-treated with lyticase and proteinase K; DNA was extracted using the High Pure PCR Template Preparation kit following the manufacturer’s instructions. The internal transcribed spacer region 1 (ITS1) and calmodulin gene were amplified by PCR and the resulting amplicons were sequenced using the Illumina MiSeq platform with pair-end reads of 150 bp. The obtained results were analyzed using the PIPITS pipeline as described by Gweon et al. [1]. Operational taxonomic units (OTU) to which less than 0.1% of the total reads attributed were disregarded. Results: Thirty-seven different OTU were identified from which two belonged to the Plantae kingdom, 11 had less than the 0.1% threshold of the total reads and were therefore disregarded. The remaining 24 different OTU (grouped in 17 phylotypes), were considered as part of the pulmonary mycobiome of patients. Two phyla were identified: Basidiomycota (33.3%) and Ascomycota (54.2%). Regarding the Basidiomycota phylum, reads were classified in three classes (Agaricomycetes, Tremellomycetes and Walleomycetes), while for the Ascomycota phylum four different taxonomical classes were identified: Pneumocystidomycetes, Dothideomycetes, Eurotiomycetes and Saccharomycetes, with the latter being the most frequent class. Twelve fungal genera were identified, being Candida the most frequently detected. The median number of fungal genera detected in patients’ pulmonary mycobiome was six (ranging from two up to nine). The genus Papilotrema and the potentially pathogenic genera Cryptococcus and Pneumocystis were exclusively found in the pulmonary mycobiome of HIV+ + patients. Other potentially pathogenic fungi such as Aspergillus spp., Trichosporon spp., Saccharomyces spp. and Schizophyllum spp. were also detected. Conclusion: This pilot study illustrates how the pulmonary mycobiome is rich and highly variable in patients with fungal infections. The obtained results suggest that the described metagenomic analysis may possess a great ability to quickly and effectively detect potentially pathogenic fungi in the mycobiome of patients, making it a promising future diagnostic tool. Thus, further optimization, standardization and clinical validation of these NGS methodologies should be warranted in the future.info:eu-repo/semantics/publishedVersio

Public policy implementation analysis: constructional and hermeneutical approaches

O presente artigo centra-se numa reflexão sobre as dificuldades analíticas de compreensão e apreensão do conjunto complexo de fatores inerentes à fase de implementação de políticas públicas. Assumindo o pressuposto de que implementação e formulação não se constituem como fases desconectadas, mas como elementos de um processo complexo de articulação entre fatores normativos e administrativos e fatores cognitivos e valorativos, assume-se a perspetiva hermenêutica como aquela que, metodológica e substantivamente, pode fornecer abordagens mais adequadas à apreensão da relativa incerteza da implementação, tendo em conta diferentes contextos, diferentes agentes e diversos valores e interesses em confluência.The present article focuses on a reflection on the analytical difficulties of understanding and apprehending the complex set of factors inherent to the implementation of public policies. Assuming that implementation and formulation are not disconnected phases, but elements of a complex process of articulation between normative and administrative factors, and cognitive and axiological factors, the hermeneutic perspective is assumed as the one that, methodologically and substantively, can provide more appropriate approaches to the apprehension of the relative uncertainty of implementation, taking into account different contexts, different actors and diverse values and interests at the confluence

Using children’s literature in ELT: A story-based approach

There has been a growing interest in the role of children’s literature in language teaching since the 80s, when the communicative approach made it possible to bring stories into the classroom (Garvie, 1990). It is undeniable that storytelling has many benefits. Not only are children naturally drawn to stories, but they are also an effective and enjoyable way to teach and learn. This article presents the findings of a MA project on using stories with children. It shows the importance of stories on language acquisition and concludes with some practical suggestions based on my teaching experience with young learners

Intertextuality and Fairy Tales in Advertising

The phenomenon of intertextuality is recurrent not only in literature, but it is also important in other fields such as advertising because it makes both the advertisement and the product more attractive for consumers. One of the most remarkable ways in which intertextuality is put into practice is through fairy tales. Since human life began, stories surrounded by magic, with fairy godmothers and magical objects have been told from generation to generation. These stories have been part of our teachings and values, which we still have nowadays, and have been transferred to different fields such as advertising. To analyse fairy tales’ intertextuality is a very interesting topic because these stories continue to play an important role in the lives of adults, teenagers, and children. In this degree final project, we will research fairy tales’ intertextuality in print advertising. We will study the effect of this intertextuality on the interpretation of advertisements and Relevance Theory will help us to explain the effects that are produced in the interpretive process

Gender, Discourse, and Desire in Twentieth-Century Brazilian Women's Literature

This study by Cristina Ferreira-Pinto explores the poetic and narrative strategies twentieth-century Brazilian women writers use to achieve new forms of representation of the female body, sexuality, and desire. Female writers discussed include: Gilka Machado, Lygia Fagundes Telles, Marcia Denser, and Marina Colasanti. While creating new forms, these writers are also deconstructing cultural myths of femininity and female behavior. In order to understand these myths, the book also presents new readings of some male-authored canonical novels by Jose de Alencar, Machado de Assis, Manuel Antonio de Almeida, and Aluisio Azevedo

Gender, Discourse, and Desire in Twentieth-Century Brazilian Women\u27s Literature

This study by Cristina Ferreira-Pinto explores the poetic and narrative strategies twentieth-century Brazilian women writers use to achieve new forms of representation of the female body, sexuality, and desire. Female writers discussed include: Gilka Machado, Lygia Fagundes Telles, Marcia Denser, and Marina Colasanti. While creating new forms, these writers are also deconstructing cultural myths of femininity and female behavior. In order to understand these myths, the book also presents new readings of some male-authored canonical novels by Jose de Alencar, Machado de Assis, Manuel Antonio de Almeida, and Aluisio Azevedo. The specific focus on female sexuality and desire acknowledges the intrinsic link between sexuality and an individual\u27s sense of identity, and its importance for female identity, given the historical repression of women\u27s bodies and the double standard of morality still pervasive in many Western cultures. In the discussion of the strategies Brazilian female poets and fiction writers employ, Ferreira-Pinto addresses some social and cultural issues that relate to a woman\u27s sense of her own body and sexuality: the characterization of women based on racial features and class hierarchy; marriage; motherhood; the silencing of the lesbian subject; and aging. Ferreira-Pinto\u27s analysis is informed by the works of various and diverse critics and theoreticians, among them Helene Cixous, Teresa De Lauretis, Adrienne Rich, Gloria Anzaldua, Georges Bataille, and Wilhelm Reich.https://docs.lib.purdue.edu/psrl/1004/thumbnail.jp

Obstructive sleep apnea associated with Diabetes mellitus Type 2: a proteomic study

Background: We previously showed that Obstructive sleep apnea (OSA), a common public health concern causing deleterious cardiometabolic dysfunction, induced proteomic alterations in red blood cells (RBC) such as changes in the redox-oligomeric state of peroxiredoxin 2 (PRDX2)1-2. Herein, we aimed to investigate whether OSA patients with Type 2 Diabetes Mellitus before and after positive airway pressure (PAP) treatment present similar changes in the RBC antioxidant protein PRDX2 to better understand the molecular basic mechanisms associated with OSA and OSA outcomes. Methods: RBC samples from control snorers (n=22 being 3 diabetics) and OSA patients before and after six month of PAP-treatment (n=29 being 8 diabetics) were analysed by non-reducing western blot using antibody against PRDX2 or PRDXSO2/3 to measure the total and overoxidized levels of monomeric/dimeric/multimeric forms of PRDX2. Results: We confirmed previously data by showing that in OSA RBC the overoxidation on the monomeric forms of PRDX2 was higher compared to controls. After PAP treatment, this overoxidation decreased followed by an increase of multimeric-overoxidized forms of PRDX2 described to be associated with chaperone protective function. In contrast, the level of PRDX2 monomers in RBC diabetic OSA, although higher abundant its overoxidation level was much lower than those observed in OSA without comorbidity and did not significant change after treatment. Moreover, the level of PAP-induced PRDX2-overoxidized-multimers was also lower in these diabetic OSA patients. The level of overoxidized monomeric/dimeric forms of PRDX2 correlated negatively with levels of insulin / triglycerides and HbA1C, respectively. After PAP, the level of (overoxidized) PRDX2SO2/3 multimers correlated positively with adrenaline levels. Conclusions: The redox/oligomeric state of RBC PRDX2 that is regulated by overoxidation of the active cysteines was differentially modulated in diabetic OSA patients compared to OSA without this comorbidity. PAP-induced overoxidized oligo forms of PRDX2 that is associated with chaperone protective function showed decreased in OSA patients with diabetes. The clinical impact of these findings needs further investigation and validation.Project partially supported by Harvard Medical School-Portugal Program (HMSP-ICJ/0022/2011), ToxOmics - Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013).info:eu-repo/semantics/publishedVersio

Adversity, depressive symptoms and suicide attempts: study of women in community and comparison between women in prison and women in the community

Dissertação de mestrado integrado em Psicologia (área de especialização em Psicologia da Justiça)A relação entre adversidade na infância, depressão e tentativas de suicídio tem sido estudada, mas nunca o foi em amostras exclusivamente femininas da comunidade. Por outro lado a literatura sugere que existe uma elevada prevalência de adversidade, depressão e tentativas de suicídio em reclusas. Este estudo teve como objetivos analisar estas variáveis em mulheres da comunidade e compará-las com um grupo de reclusas, analisando o contributo da adversidade para a depressão e tentativas de suicídio. Um total de 394 mulheres (211 reclusas e 183 da comunidade) completou o Adverse Childhood Experiences Study Questionnaire e a sub-escala da depressão do Psychopathological Symptom Inventory. As mulheres na comunidade relatam muitas experiências adversas e estas são preditoras da sintomatologia depressiva e das tentativas de suicídio. As reclusas relataram mais abuso sexual, abuso de substâncias na família, familiares em reclusão e divórcio dos pais, enquanto as da comunidade relataram mais violência doméstica. Não há diferenças entre os dois grupos quanto ao total de experiências adversas. A depressão e as tentativas de suicídio são superiores nas reclusas. Nos dois grupos a adversidade é preditora tentativas de suicídio. Programas de prevenção comunitários e projetos prisionais que considerem a história de vida e problemas específicos são necessários em contexto prisional.The relationship between childhood adversity, depression and suicide attempts has been studied, but never in exclusively female community samples. On the other hand the literature suggests that there is a high prevalence of adversity, depression and suicide attempts in female inmates. This study aimed to analyze these variables in women from the community and compare them with a group of inmates, analyzing the contribution of adversity to depression and suicide attempts. A total of 394 women (211 inmates and 183 from community) completed the Adverse Childhood Experiences Study Questionnaire and the depression subscale of the Psychopathological Symptom Inventory. Women in the community reported many adverse experiences and these are predictors of depressive symptoms and suicide attempts. Female inmates reported more sexual abuse, substance abuse in the family, family members in prison and divorced parents, while the community reported more domestic violence. There are no differences between the two groups in the total number of adverse experiences. Depression and suicide attempts are higher among inmates. In both groups the adversity predicts suicide attempts. Prevention community programs and projects that consider life history and specific problems are needed in the prison context