The Study of Dynamic Monitor of Rice Drought in Jiangxi Province with Remote Sensing

AbstractMODIS sensor has a high temporal resolution, spectral resolution, and moderate spatial resolution. The data products are widely used in Large-scale and long period dynamic monitoring of drought. This paper focuses on characters of farming rice dominated in Jiangxi province, and analysis on remote sensing monitoring model and drought monitoring indicators which selection the MODIS data products and drought information related from 2000-2008. Established standard for classification of drought in Jiangxi province, and verified instances of 2003 drought in Jiangxi. This study may have a certain significance to agricultural drought monitoring based on rice planting in southern China, because the correspondencematch between the selected TD indices and cloud cover with precipitation and daily maximum temperature evolution curve

MicroRNA roles in beta-catenin pathway

β-catenin, a key factor in the Wnt signaling pathway, has essential functions in the regulation of cell growth and differentiation. Aberrant β-catenin signaling has been linked to various disease pathologies, including an important role in tumorigenesis. Here, we review the regulation of the Wnt signaling pathway as it relates to β-catenin signaling in tumorigenesis, with particular focus on the role of microRNAs. Finally, we discuss the potential of β-catenin targeted therapeutics for cancer treatment

The Research on Relation Between Land Resource Utilization and Water and Soil Conservation in Developing the West of China

Abstract: This paper is according to the question about the land resources utilization and the situation of soil and water erosion in the west under today's social economy conditions,discussing what is reasonable land utilization and what is unreasonable land utilization. We think with the development of the social economy land resources would be tend to multiplex. At the beginning of exploiting and using land resources, the key to resolve the land resources reasonable use is to the controlling work of soil and water conservation

Computational micromechanics-based prediction of the failure of unidirectional composite lamina subjected to transverse and in-plane shear stress states

This paper presents a micromechanics-based 3D finite element model for predicting the damage initiation, propagation, and failure strength of TC33/Epoxy carbon fiber reinforced polymer (CFRP) unidirectional lamina under biaxial loadings. The finite element model is generated by introducing representative volume element (RVE) with a random distribution of fibers and a non-zero thickness, numerically identified interface phase via cohesive elements. In the finite element model, the carbon fibers are considered as elastic, while the elasto-plastic behavior and damage of the matrix are governed by extended Drucker–Prager plastic yielding model and ductile damage criterion. By imposing periodic boundary conditions to the RVEs, various cases subjected to uniaxial and biaxial loading conditions are carried out. During the combined transverse and in-plane shear stress states, a failure transition from compression- or tension-dominated to shear-dominated is captured, and the effects of the interfacial strength on the transition damage mechanisms are discussed. The corresponding failure locus is compared with the upper bound and lower bound predictions of three phenomenological failure criteria (Hashin, Tasi–Wu, and Puck failure criteria) for composites. It was found that in the interface-dominated failure of a CFRP lamina with a weak interface, the Hashin failure criterion performs best among the currently popular failure criteria. However, in the matrix-dominated failure with a strong interface, the Puck failure criterion performs best. Comparing these three criteria, it can be seen the Tsai–Wu may be generally better than both of others as it presents more neutral predictions in both of the examined cases

p53 inhibits CTR1-mediated cisplatin absorption by suppressing SP1 nuclear translocation in osteosarcoma

BackgroundOsteosarcoma (OS) is a malignant bone tumor mainly affecting children and young adolescents. Cisplatin is a first-line chemotherapy drug for OS, however, drug resistance severely limits the survival of OS. Nevertheless, cellular factors in cisplatin resistance for OS remain obscure. In this study, the function and potential mechanism of p53 in cisplatin absorption were explored in OS cells.MethodsThe CRISPR-Cas9 gene editing technology was performed to obtain p53 gene knock-out U2OS cells. The p53 over-expression 143B cell line was established by lentivirus-mediated virus infection. Moreover, the functions of p53 and CTR1 in cisplatin absorption were assessed by inductively coupled plasma mass spectrometry (ICP-MS) through CTR1 over-expression and knock-down. Further, the DNA binding activity of SP1 on CTR1 gene promoter was determined by dual-luciferase assay and chromatin immunoprecipitation (ChIP) assay. The functional regulation of p53 on SP1 was studied by nucleocytoplasmic separation assay and electrophoretic mobility shift assay (EMSA). The interaction between p53 and SP1 was verified by Co-Immunoprecipitation assay.ResultsUnder cisplatin treatment, p53 knock-out promoted CTR1 expression and cisplatin uptake, while p53 overexpression inhibited CTR1 expression and cisplatin uptake. Moreover, p53 regulated CTR1 level not by binding to CTR1 promoter directly but by suppressing the nuclear translocation of transcription factor specificity protein 1 (SP1). It was verified that SP1 is directly bound with CTR1 promoter. SP1 overexpression stimulated CTR1 expression, and SP1 knock-down attenuated CTR1 expression.ConclusionThe p53 might function as a negative regulator in CTR1 mediated cisplatin absorption, and the p53-SP1-CTR1 axis is a target for cisplatin resistance

Evaluation of ferritin and TfR level in plasma neural-derived exosomes as potential markers of Parkinson’s disease

IntroductionEarly diagnosis of Parkinson’s disease (PD) remains challenging. It has been suggested that abnormal brain iron metabolism leads to excessive iron accumulation in PD, although the mechanism of iron deposition is not yet fully understood. Ferritin and transferrin receptor (TfR) are involved in iron metabolism, and the exosome pathway is one mechanism by which ferritin is transported and regulated. While the blood of healthy animals contains a plentiful supply of TfR-positive exosomes, no studies have examined ferritin and TfR in plasma neural-derived exosomes.MethodsPlasma exosomes were obtained from 43 patients with PD and 34 healthy controls. Neural-derived exosomes were isolated with anti-human L1CAM antibody immunoabsorption. Transmission electron microscopy and western blotting were used to identify the exosomes. ELISAs were used to quantify ferritin and TfR levels in plasma neural-derived exosomes of patients with PD and controls. Receivers operating characteristic (ROC) curves were applied to map the diagnostic accuracy of ferritin and TfR. Independent predictors of the disease were identified using logistic regression models.ResultsNeural-derived exosomes exhibited the typical exosomal morphology and expressed the specific exosome marker CD63. Ferritin and TfR levels in plasma neural-derived exosomes were significantly higher in patients with PD than controls (406.46 ± 241.86 vs. 245.62 ± 165.47 ng/μg, P = 0.001 and 1728.94 ± 766.71 vs. 1153.92 ± 539.30 ng/μg, P < 0.001, respectively). There were significant positive correlations between ferritin and TfR levels in plasma neural-derived exosomes in control group, PD group and all the individuals (rs = 0.744, 0.700, and 0.752, respectively). The level of TfR was independently associated with the disease (adjusted odds ratio 1.002; 95% CI 1.000–1.003). ROC performances of ferritin, TfR, and their combination were moderate (0.730, 0.812, and 0.808, respectively). However, no relationship was found between the biomarkers and disease progression.ConclusionIt is hypothesized that ferritin and TfR in plasma neural-derived exosomes may be potential biomarkers for PD, and that they may participate in the mechanism of excessive iron deposition in PD