18 research outputs found
Effect of silver nanoparticles on performance, N metabolism and microbial profile of broiler chicken
Nano-nutrition of chicken embryos. The effect of silver nanoparticles and glutamine on molecular responses, and the morphology of pectoral muscle:the effect of silver nanoparticles and glutamine on molecular responses, and the morphology of pectoral muscle
Influence of hydrocolloidial silver nanoparticles on gastro-intestinal microflora and immune status of broiler chickens
Influence of silver nanoparticles on infection with <em>Campylobacter jejuni</em> in broiler chickens
Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart
Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry nutrition, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in two concentrations (10 and 20 ppm). After dissection of the birds, breast muscles and hearts were collected. Gene expression of FGF2 and VEGFA on the mRNA and protein levels were evaluated using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay methods. The results for gene expression in the breast muscle revealed changes on the mRNA level (FGF2 was up-regulated, P < 0.05) but not on the protein level. In the heart, 20 ppm of silver nanoparticles in drinking water increased the expression of VEGFA (P < 0.05), at the same time decreasing FGF2 expression both on the transcriptional and translational levels. Changes in the expression of these genes may lead to histological changes, but this needs to be proven using histological and immunohistochemical examination of tissues. In general, we showed that AgNano application in poultry feeding influences the expression of FGF2 and VEGFA genes on the mRNA and protein levels in growing chicken